2025-12-10 REL PDBe PDBe PDBe 2025-03-10 2025-11-26 2025-11-26 2025-12-10 iNEXT-Discovery 24433 European Union Cryo-EM map of P. furiosus 70S grown at 95 degC, focused on the ssu head Matzov D Georgeson J Westhof E Schwartz S Shalev-Benami M RNA modification, cryo-EM, ribosome Garcia-Campos MA Georgeson J Nir R Reichelt R Fluke KA Matzov D Iyer V Burkhart BW Lui L Kustanovich A Grunberger F Thalalla-Gamage S Howpay-Manage SA Gerovac M Alexandre N Nobe Y Nowak JS Perera M Apostle A Fang S Glatt S Jona G Ferreira-Cerca S Vogel J Taoka M Meier JL Westhof E Santangelo TJ Grohmann D Shalev-Benami M Schwartz S Pan-modification profiling facilitates a cross-evolutionary dissection of the thermoregulated ribosomal epitranscriptome. Cell 188 6825 6844.e28 2025 41130207 doi:10.1016/j.cell.2025.09.014 1097-4172 EMD-53068 consensus EM volume EMD-53069 focused EM volume EMD-53070 focused EM volume EMDB EMD-53068 consensus EM volume EMDB EMD-53069 focused EM volume EMDB EMD-53070 focused EM volume EMDB EMD-53071 associated EM volume P. furiosus 70S P. furiosus 70S 0 Pyrococcus furiosus singleParticle particle 7.5 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 3.0 GATAN K3 (6k x 4k) 1.01 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 4V6U 2.76 FSC 0.143 CUT-OFF 169992 PROJECTION MATCHING PROJECTION MATCHING emd_53071.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 448 448 448 0 0 0 448 448 448 443.52002 443.52002 443.52002 90.0 90.0 90.0 X Y Z -1.5259297 2.5110037 0.0010132099 0.06252973 0.99000007 0.99000007 0.99000007 0.36 AUTHOR Sharpened Cryo-EM map of P. furiosus 70S grown at 95 degC, focused on the ssu head emd_53071_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 448 448 448 0 0 0 448 448 448 443.52002 443.52002 443.52002 90.0 90.0 90.0 X Y Z -0.3459491 0.93705404 0.0010132099 0.027585413 0.99000007 0.99000007 0.99000007 AUTHOR Cryo-EM map of P. furiosus 70S grown at 95 degC, focused on the ssu head emd_53071_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 448 448 448 0 0 0 448 448 448 443.52002 443.52002 443.52002 90.0 90.0 90.0 X Y Z -0.83797306 1.1239403 0.0018676398 0.107339725 0.99000007 0.99000007 0.99000007 AUTHOR Cryo-EM map of P. furiosus 70S grown at 95 degC, focused on the ssu head - halfmap A emd_53071_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 448 448 448 0 0 0 448 448 448 443.52002 443.52002 443.52002 90.0 90.0 90.0 X Y Z -0.89882505 1.1858274 0.0018491546 0.10706209 0.99000007 0.99000007 0.99000007 AUTHOR Cryo-EM map of P. furiosus 70S grown at 95 degC, focused on the ssu head - halfmap B