2025-12-10RELPDBeINITIAL_RELEASEREPOSITORYDATABASE_REFERENCEScitationem_admin_citation.journal_volume_citation.page_first_em_admin.last_updatePDBePDBe2025-03-112025-11-052025-11-052025-12-10iNEXT-Discovery24433European UnionStructure of P. furiosus 70S ribosome grown at 102degMatzov DGeorgeson JWesthof ESchwartz SShalev-Benami MRNA modification, cryo-EM, ribosomeGarcia-Campos MAGeorgeson JNir RReichelt RFluke KAMatzov DIyer VBurkhart BWLui LKustanovich AGrunberger FThalalla-Gamage SHowpay-Manage SAGerovac MAlexandre NNobe YNowak JSPerera MApostle AFang SGlatt SJona GFerreira-Cerca SVogel JTaoka MMeier JLWesthof ESantangelo TJGrohmann DShalev-Benami MSchwartz SPan-modification profiling facilitates a cross-evolutionary dissection of the thermoregulated ribosomal epitranscriptome.Cell1886825202541130207doi:10.1016/j.cell.2025.09.0141097-4172EMD-53072consensus EM volumeEMD-53073focused EM volumeEM map that was focus refined on the large ribosomal subunitEMD-53074focused EM volumeEM map that was focus refined on the body of the small ribosomal subunitEMD-53076focused EM volumeEM map that was focus refined on the head of the small ribosomal subunit9qf5FULLOVERLAPEMDBEMD-53099associated EM volumeStructure of P. furiosus 70S ribosome grown at 102degEMDBEMD-53072consensus EM volumeEMDBEMD-53073focused EM volumeEM map that was focus refined on the large ribosomal subunitEMDBEMD-53074focused EM volumeEM map that was focus refined on the body of the small ribosomal subunitEMDBEMD-53076focused EM volumeEM map that was focus refined on the head of the small ribosomal subunitP. furiosus 70SP. furiosus 70S0123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960616263Pyrococcus furiosus16S rRNAPyrococcus furiosus0.4892134061AUUC(4AC)GG(OMU)UGAUCCUG(5MC)CGGAGGCCACUGCUAUGGGGGUC(4AC)GACUAAGCCA(OMU)GCGAGUCA
AGGGGGCGUCCCUUCUGGGACGCCAC(4AC)GGCGGACGGCUCAGUAACACGUCGGUAAC(OMC)UACCCUCGGGAGGGG
GAUAACCC(4AC)GGGAAACUGGG(OMG)CUAAUCCCCCAUAGGCCUGGGGUACUGGAAGGUCCCCAGGC(4AC)GAAAG
GGAGCCGUAAGGCUC(4AC)GCC(4AC)GAGGAU(OMG)(OMG)G(5MC)(4AC)GGCGGC(LHH)GAUUAGGUAGUUGG
UGGGGUAACGGCCCACCAAG(5MC)(4AC)GAAGAUCGGUACGGGC(4AC)GUGAGAGCGGGAGCC(4AC)GGAGAUGGA
CACUGAGACACG(OMG)GUCCAGGCCCUACGGGGCGCAGCAGGCGCGA(A2M)ACCUC(4AC)GCAAUGCGGGAAAC
(4AC)GCGACGGGGGGACCCCCAGUGC(4AC)GUGCCUCUGGCACGGCUUU(OMU)(OMC)(4AC)GGAGUGUAAAAAGC
UC(4AC)GGGAAUAAGG(OMG)CUG(OMG)GCAAGG(5MC)(4AC)GGUGG(5MC)AGCCGCC(G7M)CGGUAAUACCGG
CGGCC(4AC)GAGUGGU(OMG)GCCACUAUUAUUGGG(5MC)CUAAAGCGG(5MC)(4AC)GUAGC(4AC)GGGCC
(4AC)GUAAGUCCCUGGCGAAAUCCCACGGCUCAAC(4AC)GUGGGGCUCGCUGGGGAUACUGCGGG(5MC)CUUGGGAC
(4AC)GGGAGAGG(5MC)(4AC)GGGGGUACCCC(4AC)GGGGUAGG(OMG)GUGAAAUCCUAUAAUCCCGGGG(OMG)G
ACCGCCAGUGG(5MC)GAAGG(5MC)GCC(4AC)GGCUGGAACGGGUC(4AC)GACGGUGAGGG(5MC)(4AC)GAAGGC
CAGGGGAGCGAAC(4AC)GGAUUAGAUACCCGGG(4SU)AGUC(4AC)(OMU)(OMG)GCUGUAAAGGA(OMU)GCGGGC
UAGGUGUCGGGCGAGCUUCGAGCUCGCC(4AC)GGUGC(4AC)GU(A2M)GGGAAG(5MC)(4AC)GUUAA(OMG)
(OMC)C(4AC)GC(4AC)GCCUGGGGAGUACGG(5MC)(4AC)GCAA(OMG)G(5MC)UGAAACUUAAAGGAAUUGGCGG
GGGAGCACUACAAGG(OMG)GUGGAGCGUGCGGUUUAAUUGGAU(5MU)CAACGCCGGGAACCUCACCGGGGG(5MC)GA
CGGCAGGAUGAAGGCCAGGCUGAAGGUCUUGCCGGACGCGCCGAGAGGA(OMG)(2MG)UGCAUGG(5MC)(5MC)G
(5MC)(4AC)GUCAGCUCGUAC(4AC)GUGAGGCGUCCACUUAAGUGUGGUAACGAGCGAGACC(4AC)GCGCCCCCAGU
UGCCAGUCCCUCC(4AC)GCUCGGGAGGGAGGCACUCUGGGGGGACUGCCGGCGAUAAGC(4AC)GGAGGAAGGGGCGGG
CGACGGUAGGUCAGUAUGCCCCGAAACCCC(4AC)GGGCUACA(5MC)GCG(OMC)GCUACAAUGGGCGGGACAAUGGGA
CC(4AC)GACCC(4AC)GAAAGGGGAAGGGAAUCCCCUAAA(OMC)C(4AC)GCCCUCAGUUCGGAU(OMC)GCGGGCUG
CAACUCGCC(4AC)GCGUGAAGCUGGAAUCCCUAGUACC(4AC)GCGUGUCAUCAUCGCGCGGCGAAUACGUCCCUG
(5MC)UCCUUGCACACAC(5MC)G(LHH)C(A1I59)G(OMU)CACUCCACCCGAGCGGAGUCCGGGUGAGGCCUGAUCU
CCUUCGGGAGGUCAGGUCGAGCCCGGGCUCCGUGAGGGGGGAGAAGUCGUA(MA6)CAAGGUAGC(4AC)GUAGGGG
(MA6)(MA6)CCUACGG(5MC)U(5MC)GAUCACCUCCUAE009950.1Small ribosomal subunit protein uS2Pyrococcus furiosus0.0230398611LEVOMADEYLVPLDQYLAAGVHIGTQQKTKDMKKFIYRVRQDGLYVLDVRKTDERLKVAGKFLAKFEPQSILAVSVRLYGQKPV
KKFGEVTGARAIPGRFLPGTMTNPAVKNFFEPDVLIVTDPRADHQAMREAVEIGIPIVALVDTENLLSYVDLAIPTNNKG
RKALALIYWILAREILYNRGEIQSREDFKIPVEEFEMKIVRRQ8U0F0Small ribosomal subunit protein uS3Pyrococcus furiosus0.0234643519999999971LEVOMAIERYFIREAVREMLIDEFLEKELRRAGYGGLDIKKTPLGTKVIIFAANPGYVIGRGGRRIRELTRILEKQFGLENPQI
EVEEIKNPYLNAKVQAVRLAQALERGIHFRRAAYAALRAIMNNGARGVEIRLSGKLTGERAKSIRFYQGYLAKVGNPAET
LVSKGYAQALLKLGVIGVKVAIMPPGARLPDEIEIIEKPVEEEVVSNEAEQ8U004Small ribosomal subunit protein eS1Pyrococcus furiosus0.0229841071LEVOMAAKRATTTRDKWKLKQWYIIYAPDFFGGVEVGLTPADDPEKVLNRVVEVTLKDVTGDFTKSHVKLYFQVYDVKGQNAYT
KFKGMKLARSYIRSLVRRKTTRIDGIFNITTKDGYKLRVMAMAIAMRRIQTSQERAIRKIMQEIIYKKAEELNFKDFVLE
SVNGKIAAEIAKEAKKIYPLRKAEIRKIKVLEEPQVIAQ8TZE1Small ribosomal subunit protein uS4Pyrococcus furiosus0.021381911LEVOMGDPKRQRKKYETPPHPWIKERLDRERVLMDKYELKNKKELWKHETQLKNFRRRARRLLAARGKQAEIEREQLLARLKRL
GLLPEDAVLDDVLSLTIEDILERRLQTIVYKKGLARTMRQARQLIVHGHIEVNGQIIRSPSYLVLKEEEDTITYARTSPF
ANPQHPERMMIEKAKQGGEAP61993Small ribosomal subunit protein eS4Pyrococcus furiosus0.0281970229999999981LEVOMARKGPKRHLKRLAAPTSWYIERKAYKWAVRPRPGPHNMRTSIPLLYIVRDYLGYAKTAREARKILNEGKFLVDGRVRKD
YKFPVGIMDVVSIPETGEHYRVLPNRIGKLILHPISEDEAFIKPLRIRNKRMIKGARVQLNFHDGTNHIVSIAEKDNYFT
SYTVLMKVPEREILEVLPFEKGAYVFVTQGKNVARKGRIVEIKRFPMGWPDVVTIEDEEGELFDTLKEYAFVVGTDKPKI
SLPQ8U011Small ribosomal subunit protein uS5Pyrococcus furiosus0.0265908911LEVOMSQEWKEYAKRVLDEWEPKTKLGMMVKEGQITDIHEIFRRGYQIKEPEIIDVLLPEVNARENQEVLDIALTVRMTDSGRR
VRFRVLAAVGNRDGYVGLGIGHGKEVGIAIRKAINYAKLNIIEIKRGCGSWECRCRRPHSVPFAVEGKEGSVRVRLIPGP
RGLGLVIGDVGKKILRLAGVQDVWSQTFGETRTTVNFAKAVFNALYNTNRVAISPEMIERYGIVVGRAMPTTFTLEQ8U017Small ribosomal subunit protein eS6Pyrococcus furiosus0.0140023299999999991LEVOMATFKLVISDPKSGIAKQVEITGAETEKLIGKRIGDQIPAKELNINLNELFGKEFPEDVKLEIRGGTDKDGFPMRPDIHG
PRRVRVLLSKGPGFRPKEKGERRKKTVRGNTISPEIVQVNVKLVYQ8U3H8Small ribosomal subunit protein uS7Pyrococcus furiosus0.0247017911LEVOMAKPLNERFFIPHEIKVMGRWSTEDVEVRDPSLKPYINLEPRLLPHTHGRHAKKHFGKANVHIVERLINKIMRSGGSHYK
VAGHFMRREHRSLNSKKVKAYEVVKEAFKIIEQRTGKNPIQVLVWAIENAAPREDTTSVMFGGIRYHVAVDISPMRRLDV
ALRNIALGASAKCYRNKMSFAEALAEEIILAANKDPKSYAYSKKLEIERIAESSRQ8U0M8Small ribosomal subunit protein uS8Pyrococcus furiosus0.0146841741LEVOMTLLDPLANALSHITNSERVGKREVYIKPASKLIGEVLRVMQKYGYIGEFEFIDDGRAGVYRVQLLGRINKAGAIKPRFP
VKVSEFEKWEKRFLPAFEFGILIVSTSQGVMSHKEAIEKGIGGRLIAYVYQ8U014Small ribosomal subunit protein eS8Pyrococcus furiosus0.0142937331LEVOMAIWQGRSLKKPSGGRIVLARKKRKRELGREPSNTRVAEQDKRKIIRTYGGNRKVRLTAAAYANVFDKSGKGRKVRIIRV
IENPANRQFARRNIITKGAIIETEIGKAKVTSRPGQDGVVNAILLEEQ8U1Y5Small ribosomal subunit protein uS9Pyrococcus furiosus0.0153179321LEVOMRIIQTTGKRKTAIARAVIREGKGRVRINGKPVEIIEPEIARFTILEPLILAGEEIWNSVDIDVKVEGGGFMGQAEAARM
AIARALVEWTGDMSLKEKFMKYDRTMLVGDPRRTEPHKPNRSTKGPRAKRQKSYRQ8U0E7Small ribosomal subunit protein uS10Pyrococcus furiosus0.0117696891LEVOMQKARIKIASTNVRSLDEVANQIKQIAERTGVRMSGPIPLPTKRIRITTRKSPDGEGSATFDRWELRVHKRLIDIEADER
AMRQIMRIRVPEDVTIEIELISP61885Small ribosomal subunit protein uS11Pyrococcus furiosus0.014756951LEVOMSEEQVNIKKKEKWGIAHIYSSFNNTIIHITDITGAETISRWSGGMVVKADRDEPSPYAAMLAARRAAEEALEKGIVGVH
IRVRAPGGSKSKTPGPGAQAAIRALARAGLKIGRVEDVTPIPHDGTRPKGGRRGRRVQ8U0E3Small ribosomal subunit protein uS12Pyrococcus furiosus0.0164776209999999981LEVOMPGKKAPNGEFAGRKLKLKRKKFRWSDIRYKRRVLRLKEKSDPLEGAPQARGIVLEKIAVEAKQPNSGMRKAVRVQLIKN
GKVVTAFCPGDGAIKFIDEHDEVIIEGIGGPKGGSMGDIPGIRYKVVKVNRVSLKELVKGRKEKPRRP61995Small ribosomal subunit protein uS13Pyrococcus furiosus0.0169489039999999971LEVOMANFRHIVRVAGVDLDGNKQLRWALTAIKGIGINFATMVCRVAGLDPFMKAGYLTDEQVKKIEEILADPVAHGIPRWAVN
RPKDYETGRDLHLITAKLDMAIREDIMRLRRIRAYRGIRHELGLPVRGQRTRSNFRRGQTVGVSRKKKQ8U0E2Small ribosomal subunit protein uS14Pyrococcus furiosus0.0066340461LEVOMAKADYNKRKPRKFGKGARRCIRCGQYGPIIRIQGLMLCRHCFREVAPKLGFRKYEQ8U013Small ribosomal subunit protein uS15Pyrococcus furiosus0.0186400251LEVOMARMHARKRGKSGSKRPPRTAPPIWVEYTVEEIENLVVKLRKEGYSTAMIGTILRDQYGIPSVKLFKDPDNPNRNLTITR
ILEKHGLAPEIPEDLMFLIRRAVNLRKHLEQHPKDLHSMRGLQLIESKIRRLVKYYKRKGKLPKNWRYDPETAKLLVRQ8TZD9Small ribosomal subunit protein uS17Pyrococcus furiosus0.0132965211LEVOMMRDIGLRVQPPAEKCDDPKCPWHGNLKIHGRVFEGIVVSDKPRKTVTVERQYYFYLNKYERYELRRSKIHAHNPPCINA
KVGDKVLIAETRPLSKTKHFVVVAVLERAEERRQ8U008Small ribosomal subunit protein eS17Pyrococcus furiosus0.0079424651LEVOMGKIRQGFIKRVARELVNKYPNEFTTDFEHNKKKVQELTNVTSKKIRNRIAGYVTKLVRMKMEGKILQ8U0U1Small ribosomal subunit protein uS19Pyrococcus furiosus0.0153573989999999991LEVOMARKEFRYRGYTLEQLMNMSLEELARLFPARQRRSLKRGLTPEQKKLLRKIRLAKKGKYKKPIRTHCRDMIILPEMVGLT
IYVHNGKEFVPVEIKPEMIGHYLGEFAPTRKKVEHGAPGVGATRSSMFVAVKQ8U002Small ribosomal subunit protein eS19Pyrococcus furiosus0.0173942361LEVOMATVYDVPGDLLVERVAQRLKEIPEIKPPEWAPFVKTGRHKERLPEQEDWWYYRVASILRRVYLDGPVGIERLRTYYGGR
KNRGHAPEKFYKAGGSIIRKALQQLEAAGFVEKVPGKGRVITPKGRSFLDKIATELKKELEEIIPELKKYQ8U0T4Small ribosomal subunit protein eS24Pyrococcus furiosus0.0116985641LEVOMEIRIKEIKENKLIGRKEIYFEIYHPGEPTPSRKDVKGKLVAMLDLNPETTVIQYIRSYFGSYISKGYAKAYDSKERMLY
IEPEYILIRDGLIEKKEGEQ8U442Small ribosomal subunit protein eS27Pyrococcus furiosus0.0068883881LEVOMAKPIIPMPRSRFLRVKCIDCGNEQIVFSHPATKVRCLICGATLVEPTGGKGIVKAKILEVLEQ8U474Small ribosomal subunit protein eS28Pyrococcus furiosus0.0081163321LEVOMAEDEGYPAEVIEIIGRTGTTGDVTQVKVRILEGRDKGRVIRRNVRGPVRIGDILILRETEREAREIKSRRQ8U159Zn-ribbon RNA-binding proteinPyrococcus furiosus0.00682400799999999951LEVOMVEMKFEIPVCTSCGREITPREHATHFICPNCGEAVIWRCESCRVLAKPYKCPNCGWEGPI6U99123S rRNAPyrococcus furiosus0.9979783751GGGGCUAGGACGCUAAG(5MC)(4AC)GCC(4AC)GGUGGAUGGCUCGGCUCGGGGCG(5MC)(4AC)GACGAAGGGCGU
GGCAAGCUGCGAUAAGCCC(4AC)GGCGAGGCGCAGGCAG(5MC)(4AC)GUCGAACCGGGGAUUCC(4AC)GAAUGGGA
CCUCC(4AC)GCGGCUUAUGC(4AC)GCACUCCGGGGUUUAUCCC(4AC)GGAGGGGGAACGCGGGGAAUUGAAACAUCU
UAGUACC(4AC)GCAGGAAAAGAAA(OMG)CAAAAGCGAUG(5MC)(4AC)GUGAGUAGGGGCGAC(4AC)GAAAACGGC
AGAGGGCAA(A2M)CUGAACCC(4AC)GGG(5MC)(4AC)GACGAGGUUCGGGGGAUGUGGGGUUGUAGGGCCCCCGUAU
GAGACCCUCGCGGGUGAAG(5MC)(4AC)GAAGUC(4AC)GCUGGAACGCGGCG(5MC)(4AC)GGAGAGGGUGAUAGCC
C(4AC)GUAGGCGUAAGCC(4AC)GCAGGGUCUCGGGGGACCCUGAGUAC(4AC)GUCGGUUGGAUAUC(4AC)GGCGGG
AAGCUGGGAGGCA(OMU)CGGCUCCCAACCCUAAAUACGUCC(4AC)GAGAC(4AC)GAUAGCGAACUAGUA(OMC)
(LHH)GUG(A2M)GGGAAAGCUGAAAAGCACCC(LHH)GGGAGGGGGGUGAAAAGAGCCUGAAACCGGGCGGCGAUAGGA
GGGUGCGGCC(4AC)GAAAGGAAUGAGCCUCCC(4AC)GAAGGAAAC(4AC)GCGGCGACGCGGGAGUACGAGGGGAGGG
GAC(LHH)GGGGUUGCAC(4AC)GUCCGUCUUGAAACACGGGGCA(OMG)GGAGUUCGCGGC(4AC)GUGGCGAGGUUAA
GGGGGUUAAGCCC(4AC)GUAGC(4AC)GCAGGGAAAC(4AC)GACAUGCC(4AC)GCAGCCGGGCUUAUGCC(4AC)GG
UGAGGGGCGGGGUGCGAAAGCGCC(4AC)GGAGUCACGGC(4AC)GCGAGACC(4AC)(OMG)AAAC(4AC)GGUCGAUC
UAGCCCGGGGCAGGGUGAA(OMG)UCCCUCAACCGAGGG(A2M)UGGAGGCC(4AC)GCUAGGGGUG(5MC)UG(A2M)U
GUGCA(OMG)UUCGCUCC(4AC)GUGACCC(LHH)GGGCUAGGGGUGAAA(OMG)(OMG)CCAAUCGAGG(5MC)(4AC)
GGAGAU(A2M)GCUGGUUCC(4AC)GC(4AC)GAAUCAUCCCGCAGGAUGGCCUC(OMC)C(4AC)GGAGGUAGGCGGUG
GGGUAGAGCACUGAUUGGAGGUGCAGGGGGCGAAAGCCCCCGGCCUCCUGUCAAACUC(4AC)GAACCCAC(4AC)GC
(4AC)GC(4AC)GUAGAUGGGGGGAGUAGGGUGGCGGUGUAAG(OMC)(4AC)GUCCAC(4AC)GAGAGGGGAACAACCC
AGAC(4AC)GGGGUUAAGGCCCCAAAGUG(5MC)(4AC)GGCUAAGUGUUACUCCAAAGGGUGUCC(4AC)GGGCCUUAG
ACAGCGGGGAGGUAGGCUUAGAAGCAGCCAUCCUUUAAAGAGUGCGUAACAGCUCACCCGUCGAGGUCCGGGGCCC
(4AC)GAAAAUGGACGGGGCUCAAGC(4AC)GGC(4AC)GC(4AC)GAGACCCCGGCGCACGGAC(4AC)GAUUGGUC
(4AC)GUGAUCGGGUAGGCGGGCGUGCCGGUGGCGUAGAAGC(4AC)GGGC(LHH)GUAAGGUC(4AC)GGUGGAGC
(4AC)GC(4AC)GGUAUCGCGGAUCCUGC(4AC)GGGAGUAGCAGCGUAGUCGGGUGAGAAUCC(4AC)GAC(4AC)GC
(4AC)GGAGGGGC(5MC)AGGGUUCCACGGCAAUGGUCGUCAGC(4AC)GUGGGUUAG(OMU)(OMC)GGUCCUAACCC
(4AC)GCC(4AC)GUAACUCGGCGCGGGGGAAAGGGAAAC(OMG)GGUUUAUAUUCC(4AC)GUAC(4AC)GCGGG
(OMG)GUAGGUGCGGCAACGCAAGCCCGGAGGGUGACGCCUCGGGGUA(OMG)GCGGAC(4AC)GGC(4AC)GAUGAGG
(5MC)(4AC)GGCUAAGCGUAUAAGCC(4AC)GGGGAGUG(5MC)(4AC)GUAAUGGCGAGAAC(4AC)GGGUGAAAGCG
CGAAUGGCCCCCCCGUUAGGGGGGUUC(4AC)GC(4AC)GAUCCCUGGGGCCCGUGAAAAGCCCUC(4AC)GGGAAUUC
(4AC)GAUCCCC(4AC)GCGAC(4AC)GUAC(4AC)GAGAAC(4AC)GACACAGGUGCCC(OMC)UGGGUGAGAAGCCUA
AGGCGUGUCGGGGGAAACC(4AC)GGC(4AC)GAGGGAACU(OMC)GGCAAACUGGCCC(4AC)GUAACUUCGGGAGAAG
GGGUG(5MC)CUGCGGGUGCGUAACC(4AC)GCAGGUCGCAGUGACUAGGGGGGCC(4AC)GA(OMC)UGUUUAAUAAAA
ACACAGGUCCCAGCUAGCC(LHH)GAAAGGGUUUGUACUGGG(OMG)(5MC)(4AC)GACGCCUGC(5MC)CAG(OMU)G
(5MC)CGGUAUGUGAAGCCCGGGUACAAC(4AC)GGGUGAAGCAC(4AC)G(OMG)UAAAC(OMG)GCGGGGGUAACUAU
AACCCUCUUAAGGU(A2M)G(OMC)GAAAUUC(5MC)UUGUCGGUUAAAUG(5MC)CGAC(5MC)UGCAUGAAUGGCGUA
ACGAGGUCCC(4AC)GCUGU(OMC)CCCGGCCGGGGCC(4AC)GGCGAAACCUCUGCCUGGCGCGCAUGCCAGGGACCCC
(4AC)GGUGGGAA(OMG)CGAAGACCCCAUGGAGCUUUACUGCAGCCUGC(4AC)GUUGCCACACGGCGGGGGGUGCGCA
GCGUAGGCGGGAGGCGUCGAAGCCCGGCCUCCGGGUCGGGUGGAGCCGUCCAUGAGACACCGCCCACUCCCCGCUGUGUG
GCUAACCCC(4AC)GAAAGGGGGGACAGCGGUAGGUGGGCAGUUUGGCUG(OMG)GGCGGCACGCCUC(4AC)GAAAAGG
UAUC(OMG)GAGGCGCCC(OMU)AAGGUCGGCUCAGGCGGGUCAGGAAU(OMC)(4AC)GC(4AC)GUAGAGUGCAAGGG
CAAAAG(5MC)(4AC)GGCCUGACUGGACC(4AC)GUAACAGAGGCGGGUCCAGCCC(4AC)GAAAGGGUGGCCU(A2M)
GCGAACCCCUGUGCCUCCC(4AC)GGUGGGGGCCAGG(OMG)AUGACAGAAAAGC(OMU)AC(OMC)CUGG(OMG)GA
(4SU)AACAGAGUCGUCUCGGGCGAGAGCCCA(OMU)AUCGACCC(4AC)GAGG(OMC)UUGCUACCU(5MC)GCUGUCG
GCUCUUCCCAUCCUGGCCCUGCAGCAGGGGCCAA(OMG)GGUGGGGG(OMU)GUUCACCCAUUAAAG(OMG)GGAACGUG
AGCUGGG(UR3)UUAGACCGUCGUGAGACAGGUCGGAUGCUAUCUA(OMC)CGGG(OMG)GUGUUGGC(4AC)GCCUGAG
(OMG)GGAAGGUGCCCCUAGUACGAGAGGAACAGGGUGC(4AC)GCGGCCUCUGGUCUA(OMC)(4AC)GGUUGUCCUCC
(4AC)GGGCAUCGCCGGGCAGCUACGC(4AC)GCAGC(4AC)GAUAAGGCCUGAAAGCAUCUAAGG(5MC)(4AC)GAAG
CGGCCCC(4AC)GAAAAUAGGCGGC(4AC)GUUCCCUGGCGCUUGGGCCUGGGCGACCGGCCCAUUGCCAGGGACGAGGG
CUCGGGUAGAAGACC(LHH)GGUUGAUAGGGCGGG(OMG)AUGUAAGCGGGAAGGGUCAAC(4AC)GACC(4AC)GCUUA
GUC(4AC)GC(4AC)GCCCCUAAUCGCC(4AC)GAGGUCCUGCCCCU5S rRNAPyrococcus furiosus0.0405892661GGUACGGCGGCCAUAGCGGGGGGGCCACACCCGGUCUCAUUUCGAACCCGGAAGUUAAGCCCCCCAGCGAUCCCGGUUGU
ACUGCCCUC(4AC)GAGAGGGGGCGGGAAGCCGGGGACGC(4AC)GC(4AC)GGCCALarge ribosomal subunit protein uL2Pyrococcus furiosus0.0260853791LEVOMGKSLIQQRRGKGSPTFKSPSHRFRGAVKYIPLNYTQEKTLRGVVEEIMHDPGRTAPVARVKFENGIEKLIIAPEGLLVG
QEIYIGPDAPIAVGNTLPLAKIPEGTYVYNIEGIPGDGGKYVRAGGTYALVVSREPDKVIVQLPSGELKAFNPMCRATIG
VVAGGGRLEKPLVKAGKAYYKYKARNKFWPTPRGVKMNAVNHPFGGKEHHPGKPTTTSRRAPPGRKVGHIAARRTGRRKQ8U001Large ribosomal subunit protein uL3Pyrococcus furiosus0.0414659691LEVOMGKVHRPRRGSLGFSPRKRAKSIVPRIRSWPKETEVRMLGFAGYKAGMTHILMIDDEPGLTNGKEIFMPVTIIETPPLRV
FGIRAYRMGYLGLETATEVIVPDFPLDNYPAKERKGKPKPKMTFYKLLERRIATLPKNYTQEMFEQKLGQLEDMIKEGEI
VDVRAIVATQPWVIKLKKKPEVMEYAIGGTSVEEKFNYIKEKLGKELRVGEVLKEGELLDVIAVTKGKGTQGPVKRWGIK
LRAHKDSKGRRKVGSIGPWHPARVMWTVPMAGQMGFHHRTELNKRLIAIGENGKLKLDENTEIEITPKGGFPHYGIVRSD
FMMIAGSVPGAIKRIIRVRPAIRPPKKKPPVQRPQITYVSVESKQQ8TZZ8Large ribosomal subunit protein uL4Pyrococcus furiosus0.0287657791LEVOMKVKVFDLNGQPVGEIELPKVFFTPFRPDLIRRAVIASWTHRIQPQGRDPMAGKRRVTENIGKGHSMARVERLKTPPRYA
AFVPFARGGRRTHPPKVEKIIWEDINKKEKRLALMSAIAATANYDLVRARGHIIDNVPQLPLIVVDDLQKVQKTRETREI
FKKLGIWDDIVRAKEKSGVRAGKGKMRGRRYKKAKGPLIVVGKNEGIVLGARNHPGVDVVVVDNLGVEHLAPGTHPGRLT
VWTVSAIERLRELYGQ8TZZ9Large ribosomal subunit protein uL5Pyrococcus furiosus0.0212698771LEVOMAVEIPNKEQILADWEAHPMRRPRIEKVTINIGVGESGERLTKAEIMLQQLTGQKPIRRKAKKTNRDFGIRRGEPIAVKV
TLRGPKAYELLKRLLAAVDNRLKASSFDEHGNVCFGIEEHINIPGVEYDPEIGIFGMDVCVTLERPGFRVARRKRKRAKI
PTRHKLTKEEGMVYMMEEFGVEIVEGQ8U012Large ribosomal subunit protein uL6Pyrococcus furiosus0.0208911889999999981LEVOMPVDAWIREEVEIPEGVEVTVEGYKVKVKGPKGELEREFFWPGIQIFTEDGNVVIYKDFPRRKDVAIARTFAAHIRNMIK
GVTEGFTYKLKVVYSHFPISVKVQGDEVIIENFLGEKAPRKAKILPGVTVKVRGQEIIVEGIDKEAVGQTAANIEQATRI
TKWDRRIFQDGIYIVEKAGKPITFQ8U015Large ribosomal subunit protein eL8Pyrococcus furiosus0.0134146642LEVOMAKPSYVKFEVPKELAEKALQAVEIARDTGKIRKGTNETTKAVERGQAKLVIIAEDVDPEEIVAHLPPLCEEKEIPYIYV
PSKKELGAAAGIEVAAASVAIIEPGKARDLVEEIAMKVKELMKQ8U160Large ribosomal subunit protein uL16Pyrococcus furiosus0.0209743611LEVOMALRPAKIDRYVDKPAYTRREYIRGAPGPKITIFDMGNPAGDFEFEVSLHTAEPVQIRQNALEAARQQVNRYLQKNVGRS
NYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKKDQKILSIRVNRQHLKFAIEGARRAAMKFPCKC
YYRIYDKEGNDVTTKILSQSLQ9UWP5Large ribosomal subunit protein uL13Pyrococcus furiosus0.0163453841LEVOMRIINAEGLILGRLASKVAKMLLEGEEVVIVNAEKAVITGNRDVIFKKYKQRTELRTLTNPRRGPFYPKRSDEIVRRTIR
GMLPWKTDRGRKAFKRLKVYVGIPKEFQGKELETIIEAHVSRLSRPKYVTVGEVAKFLGGKFQ8U0E6Large ribosomal subunit protein uL14Pyrococcus furiosus0.0152648659999999981LEVOMAKKGAGATRGVSAVRPTRALPVGAYLTVADNSGAKVIQIIGVVEYHGTRRRLASAGVGDMVVATVKKGRPDMRHQVVRA
VIIRQRKEYRRLDGMRVKFEDNAAVIVTPEGVPRGTEIRGPVAREAAERWVRIGSIASIIVQ8U009Large ribosomal subunit protein eL14Pyrococcus furiosus0.0089796592LEVOMPAIDVGRIAVVIAGRRAGQKVVVVDIIDKNFVLVTGAGLNKVKRRRMNIKHIEPLPQKIDIPRGASDEEVRAALEKAGI
SLAQ8U2L5Large ribosomal subunit protein uL15Pyrococcus furiosus0.0164393461LEVOMIRRRKKVRKLRGSHTHGWGCKKKHRGGGSKGGRGMAGTGKRNKSKWTWTIKYAPDHLGKRGFSRPPEVQREVRVVNLKF
IDEHLDELMQMGIAYEEGGKIIVDVTQFADKVLGTGKLTRPLVIKARAFSPKAEEKIKAAGGEAVLVQ8U018Large ribosomal subunit protein eL15Pyrococcus furiosus0.0226448961LEVOMGMYKYIREAWKSPKKSYVGELLKQRMIKWRREPVVVRIERPTRLDRARALGYQAKQGYVIVRVRVRKGGRKRPRWKGGR
KPSKMGQVKYSPKKSLQWIAEEKAARKFPNLEVLNSYWVGEDGMYKWFEVIMVDPHHPVIKSDPKIAWIALKHHKGRVFR
GLTSAGKKGRGLRNKGKGAEKVRPSIRANEGKGKQ8U2F9Large ribosomal subunit protein uL18Pyrococcus furiosus0.0232338689999999971LEVOMAHGPRYRVPFRRRREGKTNYRKRLKLLKSGKPRLVVRKSLNHHIAQIIVYDPKGDRTLVSAHTRELIRDFGWKGHCGNT
PSAYLLGLLIGYKAKQAGIEEAILDIGLHPPVRGSSVFAVLKGAVDAGLNVPHSPEIFPDEYRIRGEHIAEYAKMLKEQD
EEKFRRQFGGYLVKGLDPEKLPEHFEEVKARIIEKFEGEGAREQ9HH80Large ribosomal subunit protein eL18Pyrococcus furiosus0.0137580369999999991LEVOMKRTGPTDPNLRRLIRYLRKKSNEYGVKIWKDVAWRLERPRRQRAEVNVSKINRYANDGEMIVVPGSVLGAGKLEKKVIV
AAWKFSETARRKIIEAGGEAITIEELIERNPTGSGVRIMEQ8U0E5Large ribosomal subunit protein eL19Pyrococcus furiosus0.01792061LEVOMNTLKMQRRIAAEILKCGENRIWIDPERIDDVASAITREDIKRLIKEGVIKKKPIKGQSRYRAKIRHEQKKKGRHRGPGS
RKGKKTARMGKKELWIKTIRALRKELRKLKAQKKIDRKTYRMLYIRAKGGQFKNKHQLYLFLEEHGLLKKQ8U016Large ribosomal subunit protein eL21Pyrococcus furiosus0.0113714491LEVOMVQKAHSFRRKTRKKLSKHPRRRGLPPLTRFLQEFEVGQRVHIVIEPSYHKGMPDPRFHGRTGTVVGKRGEAYIVEIKDG
SKVKTLFIHPVHLRPQKQ8U217Large ribosomal subunit protein uL22Pyrococcus furiosus0.0176798181LEVOMAKRFGYSFQNFDPKRMARASARDLRISPKLAVEVCRELRGMMLNDALRYLDDVIALKRPVPLKRYNDSQGHKPGKGFGP
GRYPVKVAKAIKKVLLNVKNNAVQKGLDPDKLKIIHIAAHKGPVLRGWYPRAFGRATPFNEQTTHIEVVVEEIRRQ8U003Large ribosomal subunit protein uL23Pyrococcus furiosus0.0098936771LEVOMDPYKVIIRPVVTEKAISLIEKENKLTFIVDRRATKQDIKRAVEEIFNVKVEKVNTLITPRGEKKAYVKLKPEYSASEVA
ARLGLFQ8U000Large ribosomal subunit protein uL24Pyrococcus furiosus0.0143649689999999981LEVOMKISSKQPRKQRKFLYNAPLHVRQKLMSAPLSRELREKYKVRNLPVRVGDKVRIMRGDYKGHEGKVVEVDLKRYRIYVEG
ATLRKTNGTEVFYPIHPSNVMIIELNLEDEKRKKIIERRAGQ8U010Large ribosomal subunit protein eL24Pyrococcus furiosus0.0079382569999999991LEVOMARWNVCSYCGKPFEPGTGKMYVRNDGRVLFFCSRKCERYYFMGRNPRKLKWTKAYQEARLQRGGEQ8U158Large ribosomal subunit protein uL29Pyrococcus furiosus0.0085492900000000011LEVOMKPSEIREMSIEEIDAKIRELRLQLAKERGLLTMGTSLENPMVIRNLRRDIARLLTIKKEKLREREKGKVKKQ8U005Large ribosomal subunit protein uL30Pyrococcus furiosus0.0177450291LEVOMAKLAVIRIRGRVNVKRPVRDTLAMLRLHRVNHCVIVDDTPSYLGMLQKAKDYITWGEINAETLAKLIRKRGRLIGNKPV
TDEYVKEKLGMTIEEFAQKVVNGEMSLKDLPNLKPVFRLHPPRGGFRGSKKRSFKEGGALGYRGEKINELIERMLQ9HH78Large ribosomal subunit protein eL30Pyrococcus furiosus0.0107275759999999991LEVOMDLAFELRKAMETGKVVLGSNETIRLAKTGGAKLIIVAKNAPKEIKDDIYYYAKLSDIPVYEFEGTSVELGTLLGKPFVV
ASLAIVDPGESKILAIAKRQ8U0M6Large ribosomal subunit protein eL31Pyrococcus furiosus0.0110832421LEVOMAIKAGEEVIFTVPIKKIKKIVPRWKRAPRAVKFVREFVARHAKAQEVIIDPKVNEKIWERGIEKPPSKLRVKVKVEEEK
REGEETVRIAYVTLAQ8U3S7Large ribosomal subunit protein eL32Pyrococcus furiosus0.0155766291LEVOMDEKEFKRLLRVRARLKRKKPRFLRQEWWRYPKFKNDPKWRRPKGIDSKMRLKLKGKPRSPSIGWSSPKLVRGLHPSGYE
EVLVHNVKELEALDPKRQAARIAHTVGKKKRIEIIKRAEELGIKVLNPRVQ9HH82Large ribosomal subunit protein eL34Pyrococcus furiosus0.0106771319999999991LEVOMKPMYRSRSWRRKYVRTPGGRVVIHFERKKPKIAHCAMCGRPLNGIPRGRPVEMRKLPKTKKRPERPMPHLCPRCMRKVM
KEQIRAQLSQ8U2L3Large ribosomal subunit protein eL33Pyrococcus furiosus0.0097555599999999981LEVOMRIKGVVLSYRRSKENQHNNVMIIKPLDVNSREEASKLIGRLVLWKSPSGKILKGKIVRVHGTKGAVRARFEKGLPGQAL
GDYVEIVQ8TZV6Large ribosomal subunit protein eL37Pyrococcus furiosus0.0072455771LEVOMGSGTATFGKRNSTPTHIRCRRCGRVSYNVKKGYCAACGFGRSRRLRKYRWSKKWKKKKNAHQ8U0P5Large ribosomal subunit protein eL43Pyrococcus furiosus0.0089566891LEVOMSGTKKVGSAGRFGARYGLKIRRRVAAVEAKMRQKHTCPVCGRKAVKRISTGIWQCQKCGATFAGGAYLPVTPAGKAVRR
ITEQ8TZI4Large ribosomal subunit protein eL39Pyrococcus furiosus0.0063237839999999991LEVOMARNKPLAKKLRLAKALKQNRRVPVWVIVKTNRRVLTHPKRRYWRRTKLKEQ8U3S6Large ribosomal subunit protein eL40Pyrococcus furiosus0.0058580631LEVOMARFPEAEARIFKKYVCLRCGATNPWGAKKCRKCGYKRLRPKAKEPRGGGRQ8U0V6Small ribosomal subunit protein eS32Pyrococcus furiosus0.0050233951LEVOMKRRPRKWKKKGRMRWKWIKKRIRRLKRQRKKERGLIQ8U232Large ribosomal subunit protein eL42Pyrococcus furiosus0.0112344491LEVOMKYPKQIRTYCPFCKKHTIHKVERVKKRPRSELSAGQRRFRRILKGYGGFPRPKPEGREKPVKKLDLRFRCTECGKAHTR
GRGFRVKKFELVEGQ8U475Large ribosomal subunit protein eL20Pyrococcus furiosus0.0093500049999999981LEVOMKVKVFRVHGYFEKNGRKFKFTKEYRGIKEEDVKELVYSDIGSKHKVKRNKIFIKEIKEIRPEEAEDIVVRRLSLELQ8U3S9C2H2-type domain-containing proteinPyrococcus furiosus0.0075971911LEVOMVKLKAIIVKDRDGEEFLRCPRCGMVFKRRKDYTKHVNKAHGWLFGRGKPKGKRLQKKYLKASQQ8U2K5ZINC ION6.5409e-0513ZNwater1.8015e-051487HOHsingleParticleparticle7.5ETHANETFS KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3000.53.0GATAN K3 (6k x 4k)1.011PHASE FLIPPING AND AMPLITUDE CORRECTION4V6U2.84FSC 0.143 CUT-OFF37459PROJECTION MATCHINGPROJECTION MATCHINGemd_53099_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
448
448448
0
00448448448443.52002443.52002443.5200290.090.090.0XYZ-0.56333811.38939690.0061604150.051470720.990000070.990000070.99000007AUTHORComposite cryo-EM map of P. furiosus 70S grown at 102 degC