<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_11_0/emdb.xsd" version="3.0.11.0" emdb_id="EMD-53142">
    <admin>
        <current_status>
            <date>2026-03-25</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <revision_history>
            <revision version="1.0" date="2026-03-25">
                <change_list>
                    <metadata>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </metadata>
                    <additional_map part="1">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </additional_map>
                    <additional_map part="2">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </additional_map>
                    <image>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </image>
                    <primary_map>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </primary_map>
                </change_list>
            </revision>
        </revision_history>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-03-14</deposition>
            <header_release>2026-03-25</header_release>
            <map_release>2026-03-25</map_release>
            <update>2026-03-25</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>European Research Council (ERC)</funding_body>
                <code>951292</code>
                <country>European Union</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-electron tomograms of Physcomitrium patens phyllids.</title>
        <authors_list>
            <author ORCID="0000-0003-2331-4638">Poege M</author>
            <author ORCID="0000-0003-4001-707X">Dickmanns M</author>
            <author ORCID="0000-0001-5514-005X">Xu P</author>
            <author ORCID="0000-0003-3931-7905">Li M</author>
            <author ORCID="0009-0008-3301-8270">Schioetz OH</author>
            <author ORCID="0000-0002-8481-6845">Kaiser COJ</author>
            <author>Ma J</author>
            <author ORCID="0000-0001-5014-6620">Bieber A</author>
            <author ORCID="0000-0002-5297-9156">Capitanio C</author>
            <author ORCID="0000-0003-1306-3564">Brenner JA</author>
            <author ORCID="0000-0002-8930-5331">Riggi M</author>
            <author ORCID="0000-0002-3636-0265">Miras M</author>
            <author ORCID="0000-0002-7842-6998">Kazemein Jasemi NS</author>
            <author ORCID="0000-0001-9957-7245">Schulze SX</author>
            <author ORCID="0000-0002-1317-7716">Simon R</author>
            <author ORCID="0000-0001-6465-0115">Frommer WB</author>
            <author ORCID="0000-0002-6402-8315">Plitzko JM</author>
            <author ORCID="0000-0001-8154-8809">Baumeister W</author>
        </authors_list>
        <keywords>Physcomitrium patens, Moss, PLANT PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Poege M</author>
                    <author order="2">Dickmanns M</author>
                    <author order="3">Xu P</author>
                    <author order="4">Li M</author>
                    <author order="5">Schioetz OH</author>
                    <author order="6">Kaiser COJ</author>
                    <author order="7">Ma J</author>
                    <author order="8">Bieber A</author>
                    <author order="9">Capitanio C</author>
                    <author order="10">Brenner JA</author>
                    <author order="11">Riggi M</author>
                    <author order="12">Miras M</author>
                    <author order="13">Kazemein Jasemi NS</author>
                    <author order="14">Schulze SX</author>
                    <author order="15">Simon R</author>
                    <author order="16">Frommer WB</author>
                    <author order="17">Plitzko JM</author>
                    <author order="18">Baumeister W</author>
                    <title>Making plant tissue accessible for cryo-electron tomography</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-53142</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Physcomitrium patens phyllids</name>
        <supramolecule_list>
            <tissue_supramolecule supramolecule_id="1">
                <name>Physcomitrium patens phyllids</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="3218">Physcomitrium patens</organism>
                    <tissue>Phyllid</tissue>
                </natural_source>
            </tissue_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>tissue</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>5.7</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>NITROGEN</cryogen_name>
                        <details>Tissue was high-pressure frozen as described in the publication and the corresponding protocol.. </details>
                    </vitrification>
                    <high_pressure_freezing>
                        <instrument>OTHER</instrument>
                        <details>The value given for _em_high_pressure_freezing.instrument is Leica EM ICE. This is not in a list of allowed values {'LEICA EM PACT2', 'EMS-002 RAPID IMMERSION FREEZER', 'BAL-TEC HPM 010', 'OTHER', 'LEICA EM PACT', 'LEICA EM HPM100'} so OTHER is written into the XML file.</details>
                    </high_pressure_freezing>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage>30</voltage>
                            <current>0.05</current>
                            <duration>10</duration>
                            <temperature units="K">90</temperature>
                            <initial_thickness>800</initial_thickness>
                            <final_thickness>300</final_thickness>
                            <details>The value given for _em_focused_ion_beam.instrument is TFS Scios/Aquilos. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.</details>
                        </focused_ion_beam>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">5.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">6.5</nominal_defocus_max>
                    <nominal_magnification>42000.0</nominal_magnification>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Bioquantum</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">2.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <number_images_used>51</number_images_used>
                </final_reconstruction>
                <ctf_correction>
                    <type>NONE</type>
                </ctf_correction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="1598358">
        <file>emd_53142.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>928</col>
            <row>928</row>
            <sec>464</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>928</x>
            <y>928</y>
            <z>464</z>
        </spacing>
        <cell>
            <a units="Å">13066.24</a>
            <b units="Å">13066.24</b>
            <c units="Å">6533.12</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-62971.805000000000291</minimum>
            <maximum>48928.504000000000815</maximum>
            <average>354.069369999999992</average>
            <std>688.703740000000039</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">14.08</x>
            <y units="Å">14.08</y>
            <z units="Å">14.08</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-53142::::</label>
        <annotation_details>This is the tomogram shown in the original publication.</annotation_details>
    </map>
    <interpretation>
        <additional_map_list>
            <additional_map format="CCP4" size_kbytes="1598358">
                <file>emd_53142_additional_2.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>928</col>
                    <row>928</row>
                    <sec>464</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>928</x>
                    <y>928</y>
                    <z>464</z>
                </spacing>
                <cell>
                    <a units="Å">13066.24</a>
                    <b units="Å">13066.24</b>
                    <c units="Å">6533.12</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-33648.069999999999709</minimum>
                    <maximum>30564.813999999998487</maximum>
                    <average>136.882839999999987</average>
                    <std>441.040070000000014</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">14.08</x>
                    <y units="Å">14.08</y>
                    <z units="Å">14.08</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-53142::::</label>
                <annotation_details>This is another tomogram example.</annotation_details>
            </additional_map>
            <additional_map format="CCP4" size_kbytes="1598358">
                <file>emd_53142_additional_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>928</col>
                    <row>928</row>
                    <sec>464</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>928</x>
                    <y>928</y>
                    <z>464</z>
                </spacing>
                <cell>
                    <a units="Å">13066.24</a>
                    <b units="Å">13066.24</b>
                    <c units="Å">6533.12</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-52331.444999999999709</minimum>
                    <maximum>23245.069999999999709</maximum>
                    <average>237.24815000000001</average>
                    <std>465.426800000000014</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">14.08</x>
                    <y units="Å">14.08</y>
                    <z units="Å">14.08</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-53142::::</label>
                <annotation_details>This is another tomogram example.</annotation_details>
            </additional_map>
        </additional_map_list>
    </interpretation>
</emd>
