2026-01-21 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update DATABASE_REFERENCES citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _em_admin.last_update PDBe PDBe 2025-04-09 2025-12-24 2025-12-24 2026-01-21 European Research Council (ERC) 101075992 European Union Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder Reif-Trauttmansdorff T Herdering E Bohn S Pascoa TC Kumar A Zimmer E Schmitz RA Schuller JM Sodium-pumping, membrane-bound, methyltransferase complex, methanogen, methanogenic, methylotrophic, archaeon, archaea, methanosarcina, methanosarcina mazei, Vitamin B12, corrinoid, cobalt, 5-hydroxybenzimidazole, small protein, oxygen-sensitive, MEMBRANE PROTEIN Reif-Trauttmansdorff T Herdering E Bohn S Pascoa T Kahnt J Zimmer E Kumar A Schmitz RA Schuller JM Structure of the Methanosarcina mazei Mtr complex bound to the oxygen-stress responsive small protein MtrI. Nat Commun UK 17 133 133 2025 41436738 doi:10.1038/s41467-025-67705-5 2041-1723 EMD-53359 consensus EM volume
Mtr consensus map
 EMD-53360 focused EM volume
Local refinement MtrA
 EMD-53358 focused EM volume
Local refinement MtrH
 9qts FULLOVERLAP EMDB EMD-53359 consensus EM volume
Mtr consensus map
 EMDB EMD-53360 focused EM volume
Local refinement MtrA
 EMDB EMD-53358 focused EM volume
Local refinement MtrH
 EMDB EMD-53361 associated EM volume
Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder
 Mtr complex Mtr complex 0 1 2 3 4 5 6 7 8 9 Methanosarcina mazei Go1 Tetrahydromethanopterin S-methyltransferase subunit A Methanosarcina mazei Go1 0.025395190999999998 3 Methanosarcina mazei Go1 LEVO MADKREPAPGWPILKGEYEVGDVKNSVLVITCGSHLPGKPILDAGAACTGSCKTENLGIEKVVAHIISNPNIRYLLVTGS EVKGHITGQSMMSLHANGVKENRIAGALGAIPYVENLNAAAVARFQEQVQVVNLLDTEDMGAITSKVRELASKDPGAFDA DPLVVEISEEGEEEEEGGVVRPVSGEIAVLRSRLKAIEARMMDIGNLNKFHSGVHAGKVEGAMIGLTITISLLGLLLLGR O59640 7.2.1.4 Tetrahydromethanopterin S-methyltransferase subunit B Methanosarcina mazei Go1 0.011879629999999999 3 Methanosarcina mazei Go1 LEVO MSIVRIAPEINLVMDTESGTVTQERKDSIQYSMEPVFERVDKLDAIADDLVNSLSPSKPLLNTWPGRENTSYIAGIYSNS FYGIIVGLAFSGLLALIIYITRLMGGVV P80655 7.2.1.4 Tetrahydromethanopterin S-methyltransferase subunit C Methanosarcina mazei Go1 0.026953893 3 Methanosarcina mazei Go1 LEVO MSAGGAGGEAKGAYPQQTLMALGIVGGLVGIYLGHFMPPAYSFFGGIGAICATVWGADAVRRVASYGLGTGVPSIGMLAL GMGILAALFGLALGGIAGPILAVVVAAIIGGVIGALANKVIGMGIPIMEQAMIEISCAGTLVILGLSVVIAGSFDYAAII ENVIANGYIALIFIIGGMGILHPFNACLGPDESQDRTLILAVEKAAIALIITGFASSLHEGLMTAGINILVGLVIWYVAF SKYYALIKRDAYAVVGTGLLPSAEELQ O59638 7.2.1.4 Tetrahydromethanopterin S-methyltransferase subunit D Methanosarcina mazei Go1 0.025279776999999996 3 Methanosarcina mazei Go1 LEVO MIDAILGNILWMVFIIIGGVLISWGVHFVPVGGAPAAMAQATGVGTGTVQLATGAGLTGLVSAGFMMNVTDNFPLIVASG AVGAMIMIAVTMIVGTWIYVYGVGCVPSSAKVKVDPITKYRQDLYVSQGTEGHGIPTVSFVSGVIGAALGGIGGSLIYYS LIEVGVSVGLERVGVTSAVTGNSLVAVAAIFAIGIFLVNAVIPSYNIGGTIEGFHDPKFKKWPKAVISSVVASILCAIVA VIAIAQLGGI P80653 7.2.1.4 Tetrahydromethanopterin S-methyltransferase subunit E Methanosarcina mazei Go1 0.035539625
c-terminal Twin-StrepTag
3 Methanosarcina mazei Go1 LEVO MEPLIGMGVLALIGVAATIAGASEDLESDIGSQSNPNSQVQLAPQMMFPHRIFNKAISGEPPSNALMCSIGAAIATVLIS EFTVSPLFALVFGSVIAASVHATFAVTATMGRCASQSRFKQPIYLDMIRSHTPAIMGYAFITTFCVLIVSYLMTVVLGHP FPLTMLAFIWGITIGAIGSSTGDVHYGAEREFQQFEFGSGLNASNSGNIVRYAESGLRNGFDNSWFCSKFGGPTTGIAFG MTVFLGSWITTIFDPAQGLSMGWLSVIAGVIIVLILIIWNWKIEVQARKAYGPYKEDKAEEASASAWSHPQFEKGGGSGG GSGGSAWSHPQFEK P80651 7.2.1.4 Tetrahydromethanopterin S-methyltransferase subunit F Methanosarcina mazei Go1 0.007573959 3 Methanosarcina mazei Go1 LEVO MAEEHEKGVPMVLAPQMGAIDATVESIRYRAQLIARNQKLDSGVAATGIIGFAAGFLFSLLMVIVLPVAVGL P80654 7.2.1.4 Tetrahydromethanopterin S-methyltransferase subunit G Methanosarcina mazei Go1 0.008030405 3 Methanosarcina mazei Go1 LEVO MDGKAPAAFVEPGEFNEVMKRLDQIDEKVEFVNSEVAQRIGKKVGRDIGILYGGVIGLLLFLIYVQISSMFM P80656 7.2.1.4 Conserved protein Methanosarcina mazei Go1 0.007623596999999999 1 Methanosarcina mazei Go1 LEVO MKCEACGRESDTKYCNDCGKVMDEVVRRVGEARWAAIDDCSFIYPLVQRVGRGEATVNDIIQALDVED Q8PUD4 Tetrahydromethanopterin S-methyltransferase subunit H Methanosarcina mazei Go1 0.03407573 6 Methanosarcina mazei Go1 LEVO MFKFDKKQEVFELGGVKFGGQPGENPTVLVSTMFYARHKIVTDEDKGIFDRAAAETLWNTQVSLSDATGLPYVNQIVGET PESIKRYIEWFVGIDDRTPFLIDSSAGNVRAAAAQYCTEIGVADRAIHNSINASIEQSEIDVLTESDVSAAIVLAFNATD PTVKGKIDILEVGGSGQTKGMLQVAKECGIKYPIIDVAAMPLGAGSGATIRSVPTLKGKFGLPIGGGYHNMASAWDWLRK FKKTQPDPKAIYMPTDIGTNLVAQIAGSDYLLYGPIENVNQIFPAVAMVDIMLGETAKELGVEIADLENHPVTKLT P80650 7.2.1.4 5-HYDROXYBENZIMIDAZOLYLCOB(III)AMIDE 0.001321326 1 B13 2-Hydroxy-archaetidylinositol 0.0008362140000000001 6 A1JAP 3-PHOSPHORYL-[1,2-DI-PHYTANYL]GLYCEROL 0.0007331369999999999 3 L1P Archaetidylinositol 0.000911277 3 A1JAO SODIUM ION 2.299e-05 3 NA Archaetidylethanolamine 0.000776205 3 A1JAN water 1.8015e-05 2641 HOH singleParticle particle 10.0 7.0 ETHANE-PROPANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 3.5 FEI FALCON IV (4k x 4k) 60.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.1 FSC 0.143 CUT-OFF cryoSPARC 150000 PROJECTION MATCHING PROJECTION MATCHING emd_53361.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 576 576 576 0 0 0 576 576 576 417.6 417.6 417.6 90.0 90.0 90.0 X Y Z 0.12179239 0.5438392 0.16043046 0.0057005165 0.725 0.725 0.725 0.2 AUTHOR composite map of mtr from consensus and local map(s)