2025-12-10 REL PDBe INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-04-09 2025-12-10 2025-12-10 2025-12-10 European Research Council (ERC) 740329 European Union European Research Council (ERC) 101054447 European Union German Research Foundation (DFG) SFB1035 Germany Novo Nordisk Foundation 31199 Denmark German Federal Ministry for Education and Research DZD Germany human PAN2-PAN3 deadenylase complex in the apo state Albrecht JC Reitinger T Basquin J Schuessler S Schaefer IB Conti E complex, mammalian, deadenylase, mRNA poly(A) tail, RNA processing, mRNA, RNA BINDING PROTEIN Albrecht JC Reitinger T Basquin J Schussler S Riggi M Schafer IB Conti E Mechanisms governing poly(A)-tail-length specificity of the human PAN2-PAN3 deadenylase complex. Cell Rep US 44 116609 116609 2025 41275497 doi:10.1016/j.celrep.2025.116609 2211-1247 9qtu FULLOVERLAP EMDB EMD-53362 associated EM volume
human PAN2-PAN3 deadenylase complex in the apo state
 heterotrimeric PAN2-PAN3 mRNA deadenylase complex composed of the regulatory PAN3 homodimer bound to the catalytic PAN2 heterotrimeric PAN2-PAN3 mRNA deadenylase complex composed of the regulatory PAN3 homodimer bound to the catalytic PAN2 0 1 2 Homo sapiens Isoform 3 of PAN2-PAN3 deadenylation complex subunit PAN3 Homo sapiens 0.092526539 2 Homo sapiens LEVO MNSGGGLPPPSAAASPSSSSLAAAVAVVAPPGVGGVPGGAAVGVKLKYCRYYAKDKTCFYGEECQFLHEDPAAGAAPGLG LHSNSVPLALAGAPVAGFPPGAVAGGGAGPPPGPKKPDLGDPGTGAAAGGGGSSGGLDGPRLAIPGMDGGALTDTSLTDS YFSTSFIGVNGFGSPVETKYPLMQRMTNSSSSPSLLNDSAKPYSAHDPLTSPASSLFNDFGALNISQRRKTPNPTASEFI PKGGSTSRLSNVSQSNMSAFSQVFSHPSMGSPATAGLAPGMSLSAGSSPLHSPKITPHTSPAPRRRSHTPNPASYMVPSS ASTSVNNPVSQTPSSGQVIQKETVGGTTYFYTDTTPAPLTGMVFPNYHIYPPTAPHVAYMQPKANAPSFFMADELRQELI NRHLITMAQIDQADMPAVPTEVDSYHSLFPLEPLPPPNRIQKSSNFGYITSCYKAVNSKDDLPYCLRRIHGFRLVNTKCM VLVDMWKKIQHSNIVTLREVFTTKAFAEPSLVFAYDFHAGGETMMSRHFNDPNADAYFTKRKWGQHEGPLPRQHAGLLPE SLIWAYIVQLSSALRTIHTAGLACRVMDPTKILITGKTRLRVNCVGVFDVLTFDNSQNNNPLALMAQYQQADLISLGKVV LALACNSLAGIQRENLQKAMELVTINYSSDLKNLILYLLTDQNRMRSVNDIMPMIGARFYTQLDAAQMRNDVIEEDLAKE VQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTEAGAPWIDLSHIISCLNKLDAGVPEKISLIS RDEKSVLVVTYSDLKRCFENTFQELIAAANGQLSAWSHPQFEKGGGSGGGSGGSAWSHPQFEK Q58A45 PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Homo sapiens 0.135523094 1 Homo sapiens LEVO MNFEGLDPGLAEYAPAMHSALDPVLDAHLNPSLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVAEVGVPVSVSH FDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGSDDIRQIQSLENGILFLTKNNLKYMARGGLIIFDYLLDENEDMHS LLLTDSSTLLVGGLQNHILEIDLNTVQETQKYAVETPGVTIMRQTNRFFFCGHTSGKVSLRDLRTFKVEHEFDAFSGSLS DFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITPLQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANP ADIFHVNPVGPLLMTFDVSASKQALAFGDSEGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPV PLTTDTLLSDWPAANSAPAPRRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDSEFDSFSQVTESPVGREE EPHLHMVSKKYRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACEL GFLFHMLDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDMQELEIPQAYRGA GGSSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPDGSKSDKTGKNYDFAQVLKRSICLDQNTQAWCD TCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRMQAEVAFKMAVKKHGGEISKNKEFALADWKELGSPEGVLVCPS IEELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPARAEEEHGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQ RKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTF IPLMLNEMPQIGDLVGLDAEFVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLT QYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFL AWYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAAVFSSVL AL Q504Q3 3.1.13.4 singleParticle particle 0.12 7.5 Quantifoil R2/1 COPPER 200 GLOW DISCHARGE AIR 0.022 ETHANE-PROPANE 95 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.62 0.5 2.8000000000000003 NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) 1 3.5 70.0 1 cryoSPARC 4 PHASE FLIPPING AND AMPLITUDE CORRECTION 1 4.6 FSC 0.143 CUT-OFF cryoSPARC 4 34171 MAXIMUM LIKELIHOOD cryoSPARC 4 MAXIMUM LIKELIHOOD cryoSPARC 4 3 cryoSPARC 4 emd_53362.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 374.52798 374.52798 374.52798 90.0 90.0 90.0 X Y Z -0.69796604 1.5456771 -0.0005740827 0.027597006 0.8512 0.8512 0.8512 0.2 AUTHOR human apo PAN2-PAN3 sharpened map AlphaFold in silico model RIGID BODY FIT emd_53362_msk_2.map emd_53362_msk_1.map emd_53362_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 374.52798 374.52798 374.52798 90.0 90.0 90.0 X Y Z -0.68875873 1.239351 0.00054211006 0.04313385 0.8512 0.8512 0.8512 AUTHOR human apo PAN2-PAN3 halfmap A emd_53362_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 374.52798 374.52798 374.52798 90.0 90.0 90.0 X Y Z -0.6061208 1.3245039 0.00053651416 0.0440731 0.8512 0.8512 0.8512 AUTHOR human apo PAN2-PAN3 halfmap B