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        <grant_support>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <code>32170029</code>
                <country>China</country>
            </grant_reference>
        </grant_support>
        <title>cryo-EM structure of TolQRA in nanodiscs</title>
        <authors_list>
            <author>Luo Y</author>
            <author>Shen C</author>
        </authors_list>
        <keywords>Molecular motor Multi-pass membrane protein Accumulates at cell constriction sites., MEMBRANE PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Shen C</author>
                    <author order="2">Xie T</author>
                    <author order="3">Luo Y</author>
                    <author order="4">Zhao F</author>
                    <author order="5">Wang X</author>
                    <author order="6">Zhang Z</author>
                    <author order="7">Pang J</author>
                    <author order="8">Zhang J</author>
                    <author order="9">Dong X</author>
                    <author order="10">Chang S</author>
                    <author order="11">Ding BS</author>
                    <author order="12">Ying B</author>
                    <author order="13">Chi W</author>
                    <author order="14">Su Z</author>
                    <author ORCID="0000-0001-8624-5591" order="15">Zhou R</author>
                    <author ORCID="0000-0001-7452-5211" order="16">Tang X</author>
                    <author ORCID="0000-0002-5022-3494" order="17">Dong H</author>
                    <title>Deciphering the molecular mechanism of the bacterial division motor TolQRA.</title>
                    <journal_abbreviation>Cell Discov</journal_abbreviation>
                    <country>UK</country>
                    <volume>11</volume>
                    <first_page>87</first_page>
                    <last_page>87</last_page>
                    <year>2025</year>
                    <external_references type="PUBMED">41184225</external_references>
                    <external_references type="DOI">doi:10.1038/s41421-025-00841-w</external_references>
                    <external_references type="ISSN">2056-5968</external_references>
                </journal_citation>
            </primary_citation>
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                <details>cryo-EM structure of TolQRA in nanodiscs</details>
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        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>cryo-EM structure of TolQRA in nanodiscs</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="511145">Escherichia coli str. K-12 substr. MG1655</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Tol-Pal system protein TolQ</name>
                <natural_source database="NCBI">
                    <organism ncbi="511145">Escherichia coli str. K-12 substr. MG1655</organism>
                </natural_source>
                <molecular_weight>
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                </molecular_weight>
                <number_of_copies>5</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="469008">Escherichia coli BL21(DE3)</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNAAAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSE
QIFYSGFKEFVRLHRANSHAPEAVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILHRQAFTVSESNKG</string>
                    <external_references type="UNIPROTKB">P0ABU9</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Tol-Pal system protein TolR</name>
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                    <organism ncbi="511145">Escherichia coli str. K-12 substr. MG1655</organism>
                </natural_source>
                <molecular_weight>
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                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="469008">Escherichia coli BL21(DE3)</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVEVDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEK
DRLERLPPEQVVAEVSSRFKANPKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI</string>
                    <external_references type="UNIPROTKB">P0ABV6</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Tol-Pal system protein TolA</name>
                <natural_source database="NCBI">
                    <organism ncbi="511145">Escherichia coli str. K-12 substr. MG1655</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.043232699</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="469008">Escherichia coli BL21(DE3)</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQ
RKMKEQQAAEELREKQAAEQERLKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKKAAAEKAAADKKAAA
EKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNAS
PAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
PKPPSQAVYEVFKNAPLDFKP</string>
                    <external_references type="UNIPROTKB">P19934</external_references>
                </sequence>
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