2026-02-04 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-05-10 2026-02-04 2026-02-04 2026-02-04 Medical Research Council (MRC, United Kingdom) United Kingdom Structural characterisation of chromatin remodelling intermediates supports linker DNA dependent product inhibition as a mechanism for nucleosome spacing. Sundaramoorthy R Hughes A Owen-hughes TA Nucleosome, Remodelling enzyme, GENE REGULATION Hughes AL Sundaramoorthy R Owen-Hughes T Structural characterisation of chromatin remodelling intermediates supports linker DNA-dependent product inhibition as a mechanism for nucleosome spacing. Elife US 14 2025 41439750 doi:10.7554/eLife.52513 2050-084X EMDB EMD-53597 associated EM volume Nucleosome-Chd1 complex Nucleosome-Chd1 complex 0 2 3
Chd1 remodeller bound to Nucleosome
Saccharomyces cerevisiae 0.4 singleParticle particle 1.5 7.5 120.0 Nacl Sodium chloride 20.0 Hepes Hepes
20mM Hepes, 120mM Nacl
 Quantifoil R2/1 COPPER/RHODIUM 400 CARBON HOLEY GLOW DISCHARGE AIR 0.0001 ETHANE 100 277 FEI VITROBOT MARK IV
Vitrified carried out in climate chamber with 100% humidity.
Purified Nucleosome-Chd1 complex.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.8 1.8 3.2 3.2 81000.0 81000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 77.0 77.0 GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 1 2562 10.0 46.0 1 2700000 cryoSPARC 4.5.3 PHASE FLIPPING ONLY 3LZ0 1 FOURIER SPACE 4.27 FSC 0.143 CUT-OFF cryoSPARC 4.5.3 129000 ANGULAR RECONSTITUTION cryoSPARC 4.5.3 ANGULAR RECONSTITUTION cryoSPARC 4.5.3 4 100000.0 cryoSPARC 4.5.3 emd_53597.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 560 560 560 0 0 0 560 560 560 308.0 308.0 308.0 90.0 90.0 90.0 X Y Z -0.004127448 1.8975159 0.0015620502 0.030215451 0.55 0.55 0.55 0.02 AUTHOR Main Map 3LZ0 PDB experimental model 6FTX PDB experimental model 7TN2 PDB experimental model FLEXIBLE FIT cross-correlation REAL 180.0 emd_53597_msk_1.map emd_53597_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 560 560 560 0 0 0 560 560 560 308.0 308.0 308.0 90.0 90.0 90.0 X Y Z -0.018916218 0.048982687 0.000082579514 0.0018827545 0.55 0.55 0.55 AUTHOR Non sharpened Main Map emd_53597_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 560 560 560 0 0 0 560 560 560 308.0 308.0 308.0 90.0 90.0 90.0 X Y Z -0.5224846 0.46912897 0.00009894266 0.051468633 0.55 0.55 0.55 AUTHOR Half Map emd_53597_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 560 560 560 0 0 0 560 560 560 308.0 308.0 308.0 90.0 90.0 90.0 X Y Z -0.45003897 0.45734474 0.00009956525 0.051421486 0.55 0.55 0.55 AUTHOR Half Map