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    <admin>
        <current_status>
            <date>2026-02-25</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <revision_history>
            <revision version="1.0" date="2026-02-25">
                <change_list>
                    <model>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                    <image>
                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </primary_map>
                </change_list>
            </revision>
        </revision_history>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-05-15</deposition>
            <header_release>2026-02-25</header_release>
            <map_release>2026-02-25</map_release>
            <update>2026-02-25</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Novo Nordisk Foundation</funding_body>
                <code>NNF21OC0070452</code>
                <country>Denmark</country>
            </grant_reference>
        </grant_support>
        <title>Paranemic crossover triangle (PXT) with 2'-Fluoro-modified pyrimidines (FY RNA)</title>
        <authors_list>
            <author>Kristoffersen EL</author>
            <author>Andersen ES</author>
            <author>Zwergius NH</author>
            <author>Andersen ES</author>
        </authors_list>
        <keywords>origami, modified nucleic acids, XNA, RNA, crossover, tetra-loop, 2'fluoro</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author ORCID="0000-0001-8965-8201" order="1">Kristoffersen EL</author>
                    <author ORCID="0009-0007-4155-2468" order="2">Zwergius NH</author>
                    <author ORCID="0009-0004-1541-4262" order="3">Vallina NS</author>
                    <author order="4">Gluck N</author>
                    <author ORCID="0000-0001-9090-211X" order="5">Stange A</author>
                    <author ORCID="0009-0004-3290-3869" order="6">Desdorf L</author>
                    <author ORCID="0009-0007-0062-8438" order="7">Civit L</author>
                    <author ORCID="0000-0003-2083-4259" order="8">Geary C</author>
                    <author ORCID="0000-0003-4128-9317" order="9">Kjems J</author>
                    <author ORCID="0000-0002-5260-2770" order="10">Valero J</author>
                    <author ORCID="0000-0002-6236-8164" order="11">Andersen ES</author>
                    <title>Serum-stable RNA origami nanodevices</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>9r7q</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-53787</accession_id>
                <content_type>associated EM volume</content_type>
                <details>Paranemic crossover triangle (PXT) with 2'-Fluoro-modified pyrimidines (FY RNA)</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Single-stranded PXT made of 2'fluoro modified pyrimidine RNA</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Single-stranded PXT made of 2'fluoro modified pyrimidine RNA</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <details>In vitro transcribed using mutant (Y639F) T7 RNA polymerase</details>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <rna macromolecule_id="1">
                <name>DNA/RNA (238-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="2086595">synthetic RNA</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.076642805</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <sequence>
                    <string>GGAA(UFT)A(UFT)(CFZ)G(UFT)(CFZ)A(UFT)GG(UFT)GA(UFT)(UFT)(CFZ)G(UFT)(CFZ)A(CFZ)
(CFZ)A(UFT)GAGG(CFZ)(UFT)AGA(UFT)(CFZ)(UFT)(CFZ)A(UFT)A(UFT)(CFZ)(UFT)AG(CFZ)G
(CFZ)(UFT)(UFT)(UFT)(CFZ)GAG(CFZ)G(CFZ)(UFT)AGAG(UFT)(CFZ)(CFZ)(UFT)(UFT)A(UFT)
(CFZ)(UFT)AG(CFZ)(CFZ)GG(UFT)(UFT)(UFT)A(UFT)A(CFZ)(UFT)(UFT)(UFT)(CFZ)GAG(UFT)G
(UFT)GAA(CFZ)(CFZ)(CFZ)GA(UFT)A(UFT)(UFT)(CFZ)(CFZ)G(CFZ)GGA(UFT)(CFZ)A(CFZ)
(UFT)A(UFT)GAG(UFT)(CFZ)G(UFT)(UFT)(CFZ)G(CFZ)GG(CFZ)(UFT)(CFZ)A(UFT)AG(UFT)
(CFZ)(CFZ)GG(CFZ)(UFT)(CFZ)AAAGGA(CFZ)A(UFT)(CFZ)A(UFT)GG(CFZ)(CFZ)(UFT)G(UFT)
(UFT)(CFZ)G(CFZ)AGG(UFT)(UFT)G(UFT)GA(UFT)(UFT)A(UFT)GAG(UFT)GAG(CFZ)(CFZ)GGG
(UFT)AAGG(CFZ)A(UFT)A(CFZ)(CFZ)G(UFT)(UFT)(CFZ)G(CFZ)GG(UFT)A(UFT)G(UFT)(CFZ)
(UFT)(UFT)A(CFZ)GA(UFT)(CFZ)(CFZ)G(CFZ)</string>
                </sequence>
            </rna>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                        <component>
                            <concentration units="mM">40.0</concentration>
                            <name>HEPES</name>
                        </component>
                        <component>
                            <concentration units="mM">5.0</concentration>
                            <formula>MgCl2</formula>
                            <name>Magnesium Chloride</name>
                        </component>
                        <component>
                            <concentration units="mM">50.0</concentration>
                            <formula>KCl</formula>
                            <name>Potassium Chloride</name>
                        </component>
                    </buffer>
                    <grid>
                        <model>Au-flat 1.2/1.3</model>
                        <material>GOLD</material>
                        <mesh>300</mesh>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">45</time>
                            <atmosphere>OTHER</atmosphere>
                            <pressure units="kPa">0.015</pressure>
                        </pretreatment>
                        <details>15 mA</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">95</chamber_humidity>
                        <chamber_temperature units="K">278</chamber_temperature>
                        <instrument>LEICA EM GP</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">50.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">0.8</nominal_defocus_min>
                    <nominal_defocus_max units="µm">1.8</nominal_defocus_max>
                    <nominal_magnification>130000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <coma_free/>
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Bioquantum</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 BIOQUANTUM (6k x 4k)</film_or_detector_model>
                            <number_grids_imaged>1</number_grids_imaged>
                            <number_real_images>4449</number_real_images>
                            <average_exposure_time units="s">1.5</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">60.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
                <startup_model type_of_model="NONE"/>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">4.41</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                    <number_images_used>434756</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="256001">
        <file>emd_53787.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>400</col>
            <row>400</row>
            <sec>400</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>400</x>
            <y>400</y>
            <z>400</z>
        </spacing>
        <cell>
            <a units="Å">258.80002</a>
            <b units="Å">258.80002</b>
            <c units="Å">258.80002</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.1005047</minimum>
            <maximum>0.24808745</maximum>
            <average>-0.00008133533</average>
            <std>0.006175731</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">0.6470001</x>
            <y units="Å">0.6470001</y>
            <z units="Å">0.6470001</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.0655</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-53787::::</label>
        <annotation_details>cryo-EM map of 2-prime-fluoro-modified pyrimidine RNA Paranemic crossover triangle (PXT) origami structure.</annotation_details>
    </map>
    <interpretation>
        <half_map_list>
            <half_map format="CCP4" size_kbytes="256001">
                <file>emd_53787_half_map_2.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>400</col>
                    <row>400</row>
                    <sec>400</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>400</x>
                    <y>400</y>
                    <z>400</z>
                </spacing>
                <cell>
                    <a units="Å">258.80002</a>
                    <b units="Å">258.80002</b>
                    <c units="Å">258.80002</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.08752137</minimum>
                    <maximum>0.19554096</maximum>
                    <average>0.00010714571</average>
                    <std>0.011886821</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">0.6470001</x>
                    <y units="Å">0.6470001</y>
                    <z units="Å">0.6470001</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-53787::::</label>
                <annotation_details>Half map B of 2-prime-fluoro-modified pyrimidine RNA Paranemic crossover triangle (PXT) origami structure</annotation_details>
            </half_map>
            <half_map format="CCP4" size_kbytes="256001">
                <file>emd_53787_half_map_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>400</col>
                    <row>400</row>
                    <sec>400</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>400</x>
                    <y>400</y>
                    <z>400</z>
                </spacing>
                <cell>
                    <a units="Å">258.80002</a>
                    <b units="Å">258.80002</b>
                    <c units="Å">258.80002</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.084160745</minimum>
                    <maximum>0.2016755</maximum>
                    <average>0.00010689569</average>
                    <std>0.011866262</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">0.6470001</x>
                    <y units="Å">0.6470001</y>
                    <z units="Å">0.6470001</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-53787::::</label>
                <annotation_details>Half map A of 2-prime-fluoro-modified pyrimidine RNA Paranemic crossover triangle (PXT) origami structure</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
