2026-03-04 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update PDBe PDBe 2025-05-19 2026-01-28 2026-01-28 2026-03-04 The Francis Crick Institute CC2075, CC2000 United Kingdom UK Research and Innovation (UKRI) CC2075, CC2000 United Kingdom Cancer Research UK CC2075, CC2000 United Kingdom Wellcome Trust CC2075, CC2000 United Kingdom Cryo-EM structure of the E3 ligase HECTD3 Esposito D Huber J Maslen S Rittinger K E3-ligase, ubiquitin, ubiquitination, HECT-ligase, LIGASE Huber J Esposito D Maslen S Chambers DO Skehel JM Rittinger K Structure and mechanism of the HECT ligase HECTD3. Nat Commun UK 2026 41690955 doi:10.1038/s41467-026-69520-y 2041-1723 Liebschner D Afonine PV Baker ML Bunkoczi G Chen VB Croll TI Hintze B Hung LW Jain S McCoy AJ Moriarty NW Oeffner RD Poon BK Prisant MG Read RJ Richardson JS Richardson DC Sammito MD Sobolev OV Stockwell DH Terwilliger TC Urzhumtsev AG Videau LL Williams CJ Adams PD Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix Acta Crystallogr D Struct Biol. 2019 31588918 doi:10.1107/S2059798319011471 0353 9r94 FULLOVERLAP EMDB EMD-53852 associated EM volume
Cryo-EM structure of the E3 ligase HECTD3
 HECTD3 ligase HECTD3 ligase 0 1 Homo sapiens E3 ubiquitin-protein ligase HECTD3 Homo sapiens 0.09744105499999998 1 Spodoptera frugiperda LEVO GPGMAGPGPGAVLESPRQLLGRVRFLAEAARSLRAGRPLPAALAFVPREVLYKLYKDPAGPSRVLLPVWEAEGLGLRVGA AGPAPGTGSGPLRAARDSIELRRGACVRTTGEELCNGHGLWVKLTKEQLAEHLGDCGLQEGWLLVCRPAEGGARLVPIDT PNHLQRQQQLFGVDYRPVLRWEQVVDLTYSHRLGSRPQPAEAYAEAVQRLLYVPPTWTYECDEDLIHFLYDHLGKEDENL GSVKQYVESIDVSSYTEEFNVSCLTDSNADTYWESDGSQCQHWVRLTMKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEG DNLKKLSDVSIDETLIGDVCVLEDMTVHLPIIEIRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVRYPRLE GTDPEVLYRRAVLLQRFIKILDSVLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPGLVAQCLRDSESSKPSFMPRLYIN RRLAMEHRACPSRDPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCP SSADTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGEEVSWSKD FPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAA MQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSR LPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDMSPWEE Q5T447 2.3.2.26 singleParticle particle 0.92 7.0 50.0 Na-P Phosphate 200.0 NaCl Sodium Chloride 0.5 TCEP 2.0 Glycerol C-flat-1.2/1.3 GOLD 400 CARBON HOLEY ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.2 3.0 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris 10 TFS FALCON 4i (4k x 4k) 25560 40.8 1 3537942 NONE 6.38 FSC 0.143 CUT-OFF cryoSPARC 75003 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC emd_53852.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 296 296 296 0 0 0 296 296 296 281.19998 281.19998 281.19998 90.0 90.0 90.0 X Y Z -0.059528895 0.24250072 0.00048485043 0.00880868 0.9499999 0.9499999 0.9499999 0.05 AUTHOR Main Map after non-uniform refinement AF-Q5T447 AlphaFold in silico model BACKBONE TRACE REAL emd_53852_msk_1.map emd_53852_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 296 296 296 0 0 0 296 296 296 281.19998 281.19998 281.19998 90.0 90.0 90.0 X Y Z -0.47400716 0.90678096 0.00074656145 0.013912731 0.9499999 0.9499999 0.9499999 AUTHOR Local resolution sharpened map used for refinement emd_53852_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 296 296 296 0 0 0 296 296 296 281.19998 281.19998 281.19998 90.0 90.0 90.0 X Y Z -0.13484657 8.474367000000001 -0.044836085 0.27393207 0.9499999 0.9499999 0.9499999 AUTHOR EMReady sharpened map emd_53852_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 296 296 296 0 0 0 296 296 296 281.19998 281.19998 281.19998 90.0 90.0 90.0 X Y Z -0.096859 0.27478373 0.0004566153 0.012760035 0.9499999 0.9499999 0.9499999 AUTHOR Half-map emd_53852_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 296 296 296 0 0 0 296 296 296 281.19998 281.19998 281.19998 90.0 90.0 90.0 X Y Z -0.10953834 0.2733501 0.0004607586 0.012706514 0.9499999 0.9499999 0.9499999 AUTHOR Half-map