2026-04-15 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update DATA_COLLECTION em_admin _em_admin.last_update EXPERIMENTAL_SUMMARY em_admin _em_admin.last_update PDBe PDBe 2025-05-29 2025-12-03 2025-12-03 2026-04-15 European Research Council (ERC) 789121 European Union European Research Council (ERC) 101141396 European Union German Research Foundation (DFG) TA157/12-1 Germany German Research Foundation (DFG) CRC1507/P18 Germany Other private UR013222 United States Structure of the Human Peptide-Loading Complex Arrested by HCMV US6 Stolz M Susac L Trowitzsch S Tampe R antigen processing, adaptive immunity, cryo-EM, ER chaperones, MHC class I, transporter associated with antigen processing, IMMUNE SYSTEM Stolz M Susac L Fahim A Keller R Saggau L Mancia F Trowitzsch S Tampe R Architectural principles of transporter-chaperone coupling within the native MHC I peptide-loading complex. Sci Adv US 12 eaea7735 eaea7735 2026 41481733 doi:10.1126/sciadv.aea7735 2375-2548 Liebschner D Afonine PV Baker ML Bunkoczi G Chen VB Croll TI Hintze B Hung LW Jain S McCoy AJ Moriarty NW Oeffner RD Poon BK Prisant MG Read RJ Richardson JS Richardson DC Sammito MD Sobolev OV Stockwell DH Terwilliger TC Urzhumtsev AG Videau LL Williams CJ Adams PD Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix Acta Crystallogr., Sect. D: Biol. Crystallogr. DK 75 861 877 2019 31588918 doi:10.1107/S2059798319011471 0907-4449 0766 ABCRE6 EMD-53326 consensus EM volume
Consensus map
 EMD-53332 focused EM volume
TAP-US6 Focus
 EMD-53334 focused EM volume
Tsn-MHC I hc-?2m Focus (EM1)
 EMD-53331 focused EM volume
MHC I hc-?2m Focus (EM2)
 EMD-53330 focused EM volume
Tsn-Tsn-dimer
 9rcv FULLOVERLAP EMDB EMD-53326 consensus EM volume
Consensus map
 EMDB EMD-53332 focused EM volume
TAP-US6 Focus
 EMDB EMD-53334 focused EM volume
Tsn-MHC I hc-?2m Focus (EM1)
 EMDB EMD-53331 focused EM volume
MHC I hc-?2m Focus (EM2)
 EMDB EMD-53330 focused EM volume
Tsn-Tsn-dimer
 EMDB EMD-53923 associated EM volume
Structure of the Human Peptide-Loading Complex Arrested by HCMV US6
 Human peptide-loading complex arrested by HCMV US6 Human peptide-loading complex arrested by HCMV US6 0 1 2 3 4 5 6 Homo sapiens Antigen peptide transporter 1 Homo sapiens Burkitt lymphoma 0.08108038299999999 1 LEVO MASSRCPAPRGCRCLPGASLAWLGTVLLLLADWVLLRTALPRIFSLLVPTALPLLRVWAVGLSRWAVLWLGACGVLRATV GSKSENAGAQGWLAALKPLAAALGLALPGLALFRELISWGAPGSADSTRLLHWGSHPTAFVVSYAAALPAAALWHKLGSL WVPGGQGGSGNPVRRLLGCLGSETRRLSLFLVLVVLSSLGEMAIPFFTGRLTDWILQDGSADTFTRNLTLMSILTIASAV LEFVGDGIYNNTMGHVHSHLQGEVFGAVLRQETEFFQQNQTGNIMSRVTEDTSTLSDSLSENLSLFLWYLVRGLCLLGIM LWGSVSLTMVTLITLPLLFLLPKKVGKWYQLLEVQVRESLAKSSQVAIEALSAMPTVRSFANEEGEAQKFREKLQEIKTL NQKEAVAYAVNSWTTSISCMLLKVGILYIGGQLVTSGAVSSGNLVTFVLYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFE YLDRTPRCPPSGLLTPLHLEGLVQFQDVSFAYPNRPDVLVLQGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGG QLLLDGKPLPQYEHRYLHRQVAAVGQEPQVFGRSLQENIAYGLTQKPTMEEITAAAVKSGAHSFISGLPQGYDTEVDEAG SQLSGGQRQAVALARALIRKPCVLILDDATSALDANSQLQVEQLLYESPERYSRSVLLITQHLSLVEQADHILFLEGGAI REGGTHQQLMEKKGCYWAMVQAPADAPE Q03518 7.4.2.14 Tapasin Homo sapiens Burkitt lymphoma 0.047673538999999994 2 LEVO MKSLSLLLAVALGLATAVSAGPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFR RYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLT VLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAA WDDDEPWGPWTGNGTFWLPRVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHF YPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTL EVAGLSGPSLEDSVGLFLSAFLLLGLFKALGWAAVYLSTCKDSKKKAE O15533 MHC class I antigen Homo sapiens Burkitt lymphoma 0.040480972999999996
HLA-B*15:10
2 LEVO MLVMAPRTVLLLLSAALALTETWAGSHSMRYFYTSVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEG PEYWDRNTQIYKAQAQTDRESLRNLRGYYNQSEAGSHTLQSMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLRSWTAA DTAAQITQRKWEAAREAEQRRAYLEGECVEWLRRYLENGKDKLERADPPKTHVTHHPISDHEATLRCWALGFYPAEITLT WQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAV VVIGAVVAAVMCRKKSSGGKGGSYSQAACSDSAQGSDVSLTA A0A678ZHF8 Beta-2-microglobulin Homo sapiens Burkitt lymphoma 0.013732547 2 LEVO MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDW SFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM P61769 Antigen peptide transporter 2 Homo sapiens Burkitt lymphoma 0.074697242 1 LEVO RPWTSLLLVDAALLWLLQGPLGTLLPQGLPGLWLEGTLRLGGLWGLLKLRGLLGFVGTLLLPLCLATPLTVSLRALVAGA SRAPPARVASAPWSWLLVGYGAAGLSWSLWAVLSPPGAQEKEQDQVNNKVLMWRLLKLSRPDLPLLVAAFFFLVLAVLGE TLIPHYSGRVIDILGGDFDPHAFASAIFFMCLFSFGSSLSAGCRGGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKT GELNSRLSSDTTLMSNWLPLNANVLLRSLVKVVGLYGFMLSISPRLTLLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVA RAGQVVREAVGGLQTVRSFGAEEHEVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQ GSLLSFMIYQESVGSYVQTLVYIYGDMLSNVGAAEKVFSYMDRQPNLPSPGTLAPTTLQGVVKFQDVSFAYPNRPDRPVL KGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDEKPISQYEHCYLHSQVVSVGQEPVLFSGSVRNNIAY GLQSCEDDKVMAAAQAAHADDFIQEMEHGIYTDVGEKGSQLAAGQKQRLAIARALVRDPRVLILDEATSALDVQCEQALQ DWNSRGDRTVLVIAHRLQTVQRAHQILVLQEGKLQKL Q03519 7.4.2.14 Unique short US6 glycoprotein Homo sapiens 0.020671127 1 Homo sapiens LEVO MDLLIRLGFLLMCALPTPGERSSRDPKTLLSLSPRQQACVPRTKSHRPVCYNDTGDCTDADDSWKQLGEDFAHQCLQAAK KRPKTHKSRPNDRNLEGRLTCQRVRRLLPCDLDIHPSHRLLTLMNNCVCDGAVWNAFRLIERHGFFAVTLYLCCGITLLV VILALLCSITYESTGRGIRRCGS P14334 ADENOSINE-5'-TRIPHOSPHATE 0.000507181 1 ATP MAGNESIUM ION 2.4305e-05 2 MG 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 2 NAG ADENOSINE-5'-DIPHOSPHATE 0.000427201 1 ADP water 1.8015e-05 4 HOH singleParticle particle 3.5 6.5 20.0 C8H18N2O4S-NaOH HEPES-sodium hydroxide 150.0 NaCl sodium chloride 10.0 MgCl2 magnesium chloride 0.05 C56H92O25 glycodiosgenin 2.5 C10H16N2O3S biotin
20 mM HEPES-NaOH pH 6.5, 150 mM NaCl, 10 mM MgCl2, 2.5 mM biotin, 0.05% (w/v) glycodiosgenin
 UltrAuFoil GOLD 300 GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV
Blot force 5, Blot time 5 s.
TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 1.5 105000.0 60241.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) 3 25454 58.0 1 726723 PHASE FLIPPING AND AMPLITUDE CORRECTION C1 2.7 OTHER
Composite map generated from 4 focus maps ranging 2.59 - 2.88 A
269213 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_53923.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 424.96 424.96 424.96 90.0 90.0 90.0 X Y Z -0.042457413 0.92385787 0.008457146 0.017306233 0.83 0.83 0.83 0.11 AUTHOR