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        <sites>
            <deposition>PDBe</deposition>
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        <key_dates>
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            <header_release>2025-12-10</header_release>
            <map_release>2025-12-10</map_release>
            <update>2026-01-21</update>
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        <grant_support>
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                <funding_body>Netherlands Organisation for Scientific Research (NWO)</funding_body>
                <code>184.034.014</code>
                <country>Netherlands</country>
            </grant_reference>
        </grant_support>
        <title>Agrobacterium phage 7-7-1 tail spike</title>
        <authors_list>
            <author>Noteborn WEM</author>
            <author>Hoeksma T</author>
            <author>Briegel A</author>
            <author>Briegel A</author>
        </authors_list>
        <keywords>Flagella phage, Agrobacterium sp. H13-3, Tail spike, VIRUS</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0003-1211-6222" order="1">Noteborn WEM</author>
                    <author ORCID="0000-0003-1628-8152" order="2">Ouyang R</author>
                    <author ORCID="0009-0004-2953-5516" order="3">Hoeksma T</author>
                    <author ORCID="0000-0002-7000-0386" order="4">Sidi Mabrouk A</author>
                    <author order="5">Esteves NC</author>
                    <author ORCID="0000-0002-8253-0851" order="6">Pelt DM</author>
                    <author ORCID="0000-0001-6271-8972" order="7">Scharf BE</author>
                    <author ORCID="0000-0003-3733-3725" order="8">Briegel A</author>
                    <title>Insights into the structure and initial host attachment of the flagellotropic bacteriophage 7-7-1.</title>
                    <journal_abbreviation>Commun Biol</journal_abbreviation>
                    <country>UK</country>
                    <volume>9</volume>
                    <first_page>55</first_page>
                    <last_page>55</last_page>
                    <year>2025</year>
                    <external_references type="PUBMED">41353512</external_references>
                    <external_references type="DOI">doi:10.1038/s42003-025-09319-7</external_references>
                    <external_references type="ISSN">2399-3642</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
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                <pdb_id>9rkv</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
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                <accession_id>EMD-54022</accession_id>
                <content_type>associated EM volume</content_type>
                <details>Agrobacterium phage 7-7-1 tail spike</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Agrobacterium phage 7-7-1</name>
        <supramolecule_list>
            <virus_supramolecule supramolecule_id="1">
                <name>Agrobacterium phage 7-7-1</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <sci_species_name ncbi="1161931">Agrobacterium phage 7-7-1</sci_species_name>
                <virus_type>VIRION</virus_type>
                <virus_isolate>SPECIES</virus_isolate>
                <virus_enveloped>false</virus_enveloped>
                <virus_empty>false</virus_empty>
            </virus_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Structural protein</name>
                <natural_source database="NCBI">
                    <organism ncbi="1161931">Agrobacterium phage 7-7-1</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.014263938</theoretical>
                </molecular_weight>
                <number_of_copies>4</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAIRFHPTNAALVACNAVVDLFDAGGSIEVYTGPQPNDPTVAVSTQTLLVTFAIPAPGYGPAELVPGQNYVRAPGALPAV
AVPGASGVAAWARVKNNAGDVIFDGDVGTSASSAFVRLSTTNLTAGVGVSVTASEYRQPTR</string>
                    <external_references type="UNIPROTKB">J7FAF8</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Structural protein</name>
                <natural_source database="NCBI">
                    <organism ncbi="1161931">Agrobacterium phage 7-7-1</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.031435197</theoretical>
                </molecular_weight>
                <number_of_copies>6</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAGIFFSGGLPCTLTVNAACGIETTGDDCPALYYPPKCNVSVEPLQMNAIISEIANAINAFGDLYDCSRLDNLKAALQKA
RSICDLPPFADAPDLDDRIAGCFDNTSSTITVQQLVNLVLSQAGGLCDLPQAGGIGDNDLIAGCIGGAEALVSLSTLRGA
LGGGSISGIIPQEVLFDQWRAGGNWEVSQSTQNRTGFIVRSLKEGIGITVSDLFVATGNGGYQSISQWEATTFLYETDAE
GTSIDGGRQQAIAIVFKRGTQWYTKSGDVILPLGSDDGVIRFRTPGYQRLTIWSGAQL</string>
                    <external_references type="UNIPROTKB">J7F9D4</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Putative tail fiber</name>
                <natural_source database="NCBI">
                    <organism ncbi="1161931">Agrobacterium phage 7-7-1</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.06155271899999999</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MVLRPVLVGGLPGCVNLPSNDDCQCWPLWSSDNAYSFGDVVQQGGVLYTANRDIPPGTPFLAGDWTVYTPGATLVPPHRE
DASYQQYQAVNFNDKLYTARTDLPPGPFDPANWQEISVEGLVEVADSSTIDFSGDGSASDPLTAAVKLDPVAGNLITATP
DGLVLLASGIPFPDPSSVNISDNVTLRVDEVTGDDATADGSLQKPYKTIVAAIGASNAGDNIEVYPGSYAGNFAPKSDTT
VTGRGFVYVDGNVTIGAAVSGVTVSGLFIDGNLTIAGANGTVEFINTTVSTSIIGTGNNTGWFLFDEVNADSVSITGAGT
GEFIFRGGIEQTDFSLNAPSKLLVENRSRVGVVNHLNGLMILKDIGVIVSLTSTAEDNAFTFLAVISSSLFDPDPRTYGA
LTKTGSCSYAFQDFARSNMAETVTGAKWLVSNGRDIHGNYDPNFYALPNGQSLNSHFEGIDNALQTLQEYSPLGRSVAVD
FGFSIIGGWSADETVGVHAVSHETVIPSNMAGSIMVTDSSTLDGVLNVMVGTSVVGTVTITDGAAVFASSEFNLDPGQLV
RIVSTSEGTFEYVGLTLVGHRQVVYVD</string>
                    <external_references type="UNIPROTKB">J7F8Z3</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
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            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
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                    <buffer>
                        <ph>7.4</ph>
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                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
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                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>LEICA EM GP</instrument>
                    </vitrification>
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                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">50.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">1.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <nominal_magnification>64000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <coma_free/>
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Bioquantum</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 BIOQUANTUM (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">40.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
                <startup_model type_of_model="OTHER"/>
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                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">3.52</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
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                        </software>
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                    <number_images_used>48966</number_images_used>
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                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
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                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
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                        </software>
                    </software_list>
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            </singleparticle_processing>
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        <label>::::EMDATABANK.org::::EMD-54022::::</label>
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