2026-04-01 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update PDBe PDBe 2025-06-17 2026-03-18 2026-03-18 2026-04-01 German Research Foundation (DFG) 406260942 Germany German Research Foundation (DFG) 442219341 Germany 13S+Beta1+Beta5 proteasome precursor complex Mark E Ramos PC Nunes MM Dohmen RJ Wendler P proteasome biogenesis, Ump1, Pba1-2, cryo-EM, HYDROLASE Mark E Ramos PC Nunes MM Matias AC Jurgen Dohmen R Wendler P Structural transitions in the stepwise assembly of proteasome core particles. Nat Commun UK 17 2026 41876489 doi:10.1038/s41467-026-70525-w 2041-1723 9rm1 FULLOVERLAP EMDB EMD-54048 associated EM volume
13S+Beta1+Beta5 proteasome precursor complex
 13S proteasome precursor complex 13S proteasome precursor complex 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Saccharomyces cerevisiae Proteasome subunit alpha type-1 Saccharomyces cerevisiae 0.02803383 1 LEVO MSGAAAASAAGYDRHITIFSPEGRLYQVEYAFKATNQTNINSLAVRGKDCTVVISQKKVPDKLLDPTTVSYIFCISRTIG MVVNGPIPDARNAALRAKAEAAEFRYKYGYDMPCDVLAKRMANLSQIYTQRAYMRPLGVILTFVSVDEELGPSIYKTDPA GYYVGYKATATGPKQQEITTNLENHFKKSKIDHINEESWEKVVEFAITHMIDALGTEFSKNDLEVGVATKDKFFTLSAEN IEERLVAIAEQD P21243 Proteasome subunit alpha type-4 Saccharomyces cerevisiae 0.028478111 1 LEVO MSGYDRALSIFSPDGHIFQVEYALEAVKRGTCAVGVKGKNCVVLGCERRSTLKLQDTRITPSKVSKIDSHVVLSFSGLNA DSRILIEKARVEAQSHRLTLEDPVTVEYLTRYVAGVQQRYTQSGGVRPFGVSTLIAGFDPRDDEPKLYQTEPSGIYSSWS AQTIGRNSKTVREFLEKNYDRKEPPATVEECVKLTVRSLLEVVQTGAKNIEITVVKPDSDIVALSSEEINQYVTQIEQEK QEQQEQDKKKKSNH P40303 Proteasome subunit alpha type-5 Saccharomyces cerevisiae 0.028649085999999997 1 LEVO MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATKEGVVLGVEKRATSPLLESDSIEKIVEIDRHIGCAMSG LTADARSMIEHARTAAVTHNLYYDEDINVESLTQSVCDLALRFGEGASGEERLMSRPFGVALLIAGHDADDGYQLFHAEP SGTFYRYNAKAIGSGSEGAQAELLNEWHSSLTLKEAELLVLKILKQVMEEKLDENNAQLSCITKQDGFKIYDNEKTAELI KELKEKEAAESPEEADVEMS P32379 Proteasome subunit alpha type-6 Saccharomyces cerevisiae 0.025634 1 LEVO MFRNNYDGDTVTFSPTGRLFQVEYALEAIKQGSVTVGLRSNTHAVLVALKRNADELSSYQKKIIKCDEHMGLSLAGLAPD ARVLSNYLRQQCNYSSLVFNRKLAVERAGHLLCDKAQKNTQSYGGRPYGVGLLIIGYDKSGAHLLEFQPSGNVTELYGTA IGARSQGAKTYLERTLDTFIKIDGNPDELIKAGVEAISQSLRDESLTVDNLSIAIVGKDTPFTIYDGEAVAKYI P40302 Proteasome subunit beta type-1 Saccharomyces cerevisiae 0.023573603999999998 1 LEVO MNGIQVDINRLKKGEVSLGTSIMAVTFKDGVILGADSRTTTGAYIANRVTDKLTRVHDKIWCCRSGSAADTQAIADIVQY HLELYTSQYGTPSTETAASVFKELCYENKDNLTAGIIVAGYDDKNKGEVYTIPLGGSVHKLPYAIAGSGSTFIYGYCDKN FRENMSKEETVDFIKHSLSQAIKWDGSSGGVIRMVVLTAAGVERLIFYPDEYEQL P38624 3.4.25.1 Proteasome maturation factor UMP1 Saccharomyces cerevisiae 0.018603623 1 Saccharomyces cerevisiae LEVO DYKDDDDKHHHHHHMNIVPQDTFKSQVSTDQDKSVLSSAVPSLPDTLRQQEGGAVPLSTQLNDRHPLESTLKNWETTQRQ RQMEQYRQIFGIAEPMKRTMEMEIVNRTDFNPLSTNGSIHRDILLNKECSIDWEDVYPGTGLQASTMVGDDVHSKIEKQL GI P38293 Proteasome chaperone 1 Saccharomyces cerevisiae 0.030718073999999998 1 LEVO MLFKQWNDLPEPKHLLDLPEISKNLQSLEVCPVPKVEFPQDLDVPQYSTAVITTKIMNPLFPKNLLQLTSIGEIKTTLTV KSPSLPQSSGKHSWNYDENFPNEVDPDQKNDTADETVYGFSFPIYSFGKTLLFSMEENFISISPIFGNMISRSIISQLAQ FSPDIIVIGTSDKIASMKVMTENECTLQPPEFITGFIGSVLTQLIVGPSKGLKFKCLVAPSEGPNGFEKLSLSDMGSLVD LCGQWLGFEPSRYSEECYRLWRCDSAAIGAQSGLYI Q05778 Proteasome assembly chaperone 2 Saccharomyces cerevisiae 0.030762895 1 LEVO MSCLVLPLVSVGNIPQLSIDWLLNSQANEWEYLEALDSKYLVEFVGPLDRPEDGSDSLYKDADMKYSSALEVFYNKKRGL FAIQQRTPLVSVNYLNNFIVEIILPFLSKYNISEICIWDSLYAMEDENGVIVRPQEVYSLGEFYFDDEAELLSNLHLNDQ ESMVNNWLHFTPTSFQDKISVDQPIFKILFQILNASQRPKALRSIKYCSCLANEGDNSLDSQQFLQWIISQKVIKNAPPI VKFVRPISWQGAYGMADARDKFVDLYN P36040 Proteasome subunit alpha type-2 Saccharomyces cerevisiae 0.027191828 1 LEVO MTDRYSFSLTTFSPSGKLGQIDYALTAVKQGVTSLGIKATNGVVIATEKKSSSPLAMSETLSKVSLLTPDIGAVYSGMGP DYRVLVDKSRKVAHTSYKRIYGEYPPTKLLVSEVAKIMQEATQSGGVRPFGVSLLIAGHDEFNGFSLYQVDPSGSYFPWK ATAIGKGSVAAKTFLEKRWNDELELEDAIHIALLTLKESVEGEFNGDTIELAIIGDENPDLLGYTGIPTDKGPRFRKLTS QEINDRLEAL P23639 Proteasome subunit alpha type-3 Saccharomyces cerevisiae 0.02874823 1 LEVO MGSRRYDSRTTIFSPEGRLYQVEYALESISHAGTAIGIMASDGIVLAAERKVTSTLLEQDTSTEKLYKLNDKIAVAVAGL TADAEILINTARIHAQNYLKTYNEDIPVEILVRRLSDIKQGYTQHGGLRPFGVSFIYAGYDDRYGYQLYTSNPSGNYTGW KAISVGANTSAAQTLLQMDYKDDMKVDDAIELALKTLSKTTDSSALTYDRLEFATIRKGANDGEVYQKIFKPQEIKDILV KTGITKKDEDEEADEDMK P23638 Probable proteasome subunit alpha type-7 Saccharomyces cerevisiae 0.031575068 1 LEVO MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAVENGTTSIGIKCNDGVVFAVEKLITSKLLVPQKNVKIQVVDRHIGCVYSG LIPDGRHLVNRGREEAASFKKLYKTPIPIPAFADRLGQYVQAHTLYNSVRPFGVSTIFGGVDKNGAHLYMLEPSGSYWGY KGAATGKGRQSAKAELEKLVDHHPEGLSAREAVKQAAKIIYLAHEDNKEKDFELEISWCSLSETNGLHKFVKGDLLQEAI DFAQKEINGDDDEDEDDSDNVMSSDDENAPVATNANATTDQEGDIHLE P21242 Proteasome subunit beta type-2 Saccharomyces cerevisiae 0.028299888999999998 1 LEVO MAGLSFDNYQRNNFLAENSHTQPKATSTGTTIVGVKFNNGVVIAADTRSTQGPIVADKNCAKLHRISPKIWCAGAGTAAD TEAVTQLIGSNIELHSLYTSREPRVVSALQMLKQHLFKYQGHIGAYLIVAGVDPTGSHLFSIHAHGSTDVGYYLSLGSGS LAAMAVLESHWKQDLTKEEAIKLASDAIQAGIWNDLGSGSNVDVCVMEIGKDAEYLRNYLTPNVREEKQKSYKFPRGTTA VLKESIVNICDIQEEQVDITA P25043 3.4.25.1 Proteasome subunit beta type-3 Saccharomyces cerevisiae 0.022627842 1 LEVO MSDPSSINGGIVVAMTGKDCVAIACDLRLGSQSLGVSNKFEKIFHYGHVFLGITGLATDVTTLNEMFRYKTNLYKLKEER AIEPETFTQLVSSSLYERRFGPYFVGPVVAGINSKSGKPFIAGFDLIGCIDEAKDFIVSGTASDQLFGMCESLYEPNLEP EDLFETISQALLNAADRDALSGWGAVVYIIKKDEVVKRYLKMRQD P25451 Proteasome subunit beta type-4 Saccharomyces cerevisiae 0.022545675999999997 1 LEVO MDIILGIRVQDSVILASSKAVTRGISVLKDSDDKTRQLSPHTLMSFAGEAGDTVQFAEYIQANIQLYSIREDYELSPQAV SSFVRQELAKSIRSRRPYQVNVLIGGYDKKKNKPELYQIDYLGTKVELPYGAHGYSGFYTFSLLDHHYRPDMTTEEGLDL LKLCVQELEKRMPMDFKGVIVKIVDKDGIRQVDDFQAQ P22141 Proteasome subunit beta type-5 Saccharomyces cerevisiae 0.031670539 1 LEVO MQAIADSFSVPNRLVKELQYDNEQNLESDFVTGASQFQRLAPSLTVPPIASPQQFLRAHTDDSRNPDCKIKIAHGTTTLA FRFQGGIIVAVDSRATAGNWVASQTVKKVIEINPFLLGTMAGGAADCQFWETWLGSQCRLHELREKERISVAAASKILSN LVYQYKGAGLSMGTMICGYTRKEGPTIYYVDSDGTRLKGDIFCVGSGQTFAYGVLDSNYKWDLSVEDALYLGKRSILAAA HRDAYSGGSVNLYHVTEDGWIYHGNHDVGELFWKVKEEEGSFNNVIG P30656 3.4.25.1 singleParticle particle 6.15 7.5
150 mM NaCl 50 mM Tris-HCl
 UltrAuFoil R1.2/1.3 GOLD 300 GOLD HOLEY ETHANE 80 277 HOMEMADE PLUNGER
manual plunge freezing device purchased from "Neptune FLuid Flow Systems".
TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.8 2.8000000000000003 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER GATAN K3 BIOQUANTUM (6k x 4k) 28764 1.0 64.0 1 5495013 cryoSPARC 4.7.0 PHASE FLIPPING AND AMPLITUDE CORRECTION 4.11 FSC 0.143 CUT-OFF cryoSPARC 4.7.0 50233 MAXIMUM LIKELIHOOD cryoSPARC 4.7.0 MAXIMUM LIKELIHOOD cryoSPARC 4.7.0 5 53819.0 cryoSPARC 4.7.0 emd_54048.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 498.0 498.0 498.0 90.0 90.0 90.0 X Y Z -0.29279327 0.47221193 -0.000027883349 0.0076881107 0.996 0.996 0.996 0.0501 AUTHOR 8RVL PDB experimental model RIGID BODY FIT REAL 39.299999999999997 emd_54048_msk_1.map emd_54048_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 498.0 498.0 498.0 90.0 90.0 90.0 X Y Z -5.1329994 4.680948 0.000016430493 0.32181168 0.996 0.996 0.996 AUTHOR emd_54048_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 498.0 498.0 498.0 90.0 90.0 90.0 X Y Z -4.639921 4.821859 0.00001703328 0.34240445 0.996 0.996 0.996 AUTHOR