2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update PDBe PDBe 2025-06-17 2026-02-04 2026-02-04 2026-03-18 Other government United Kingdom Other private United Kingdom Hexameric AAV2 Rep40-ssDNA (ITR) complex in presence of ATPyS Rouse SL Bubeck D Barritt JD Xu V Wake M Helicase, Adeno-associated Virus, AAA+ ATPase, DNA BINDING PROTEIN Xu V McInnes A Wake M Acebron-Garcia-de-Eulate M Barritt JD Bubeck D Rouse SL Structural basis for Rep-mediated adeno-associated virus packaging. Cell Rep US 45 117044 117044 2026 41790558 doi:10.1016/j.celrep.2026.117044 2211-1247 9rm5 FULLOVERLAP EMDB EMD-54050 associated EM volume
Hexameric AAV2 Rep40-ssITR complex in presence of ATPgS
 AAV2 Hexameric Rep40-ssITR in presence of ATPgS AAV2 Hexameric Rep40-ssITR in presence of ATPgS 0 1 2
Adeno-associated Virus 2 Replication protein 40 in complex with inverted-terminal-repeat ssDNA and ATP-gamma-S
adeno-associated virus 2 Protein Rep40 adeno-associated virus 2 0.04414892199999999
Latch-WTAAV2Rep40-TrB-3xFLAG-TEV-6xHis
6 Escherichia coli LEVO EQNKENQNPNSDAPVIRSKTSARYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTA PDYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGKTNIAEAIAHTVPFYGCVNWT NENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKVRVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQP LQDRMFKFELTRRLDHDFGKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPSTSDA EASINYADRLARGHSLGSLVPRGSGGGGSGGGGSGGGGSMDYKDHDGDYKDHDIDYKDDDDKGSENLYFQSHHHHHH Q89269 3.6.4.12 DNA adeno-associated virus 2 0.0028468609999999996 1 (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) DNA PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER 0.0005232469999999999 4 AGS MAGNESIUM ION 2.4305e-05 5 MG ADENOSINE-5'-DIPHOSPHATE 0.000427201 2 ADP singleParticle particle 0.8 7.8 200.0 NaCl Sodium Chloride 25.0 (HOCH2)*3CNH2 Tris 1.0 C9H15O6P TCEP 1.0 C3H8O3 Glycerol UltrAuFoil R1.2/1.3 GOLD 300 PLASMA CLEANING ETHANE 100 295.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.6 2.4 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER EPU 3.8.1 GATAN K3 (6k x 4k) 5760 4092 1.68 39.99 1 cryoSPARC 4.4.1 PHASE FLIPPING AND AMPLITUDE CORRECTION C1 2.44 FSC 0.143 CUT-OFF cryoSPARC 4.4.1 91099 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD cryoSPARC 4.4.1 emd_54050.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 297.0 297.0 297.0 90.0 90.0 90.0 X Y Z -0.10550567 0.33213812 -0.00007065609 0.0072635706 0.825 0.825 0.825 0.0393 AUTHOR AlphaFold in silico model ISOLDE 1.8 emd_54050_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 297.0 297.0 297.0 90.0 90.0 90.0 Z Y X -14.6469965 40.887990000000002 -0.00000000000396 1.0 0.825 0.825 0.825 AUTHOR Phenix auto-sharpened map emd_54050_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 297.0 297.0 297.0 90.0 90.0 90.0 X Y Z -0.32430246 0.38338077 0.0001664586 0.036699887 0.825 0.825 0.825 AUTHOR emd_54050_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 297.0 297.0 297.0 90.0 90.0 90.0 X Y Z -0.32804692 0.397051 0.00016586411 0.035967767 0.825 0.825 0.825 AUTHOR