2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-06-26 2026-03-18 2026-03-18 2026-03-18 Other government United Kingdom Other private United Kingdom Focused map of 3 subunits of Rep40 +dsDNA (ITR) in complex with ATPgS Rouse SL Bubeck D Barritt JD Xu V Wake M Helicase, Adeno-associated Virus, AAA+ ATPase, DNA BINDING PROTEIN Xu V McInnes A Wake M Acebron-Garcia-de-Eulate M Barritt JD Bubeck D Rouse SL Structural basis for Rep-mediated adeno-associated virus packaging. Cell Rep US 45 117044 117044 2026 41790558 doi:10.1016/j.celrep.2026.117044 2211-1247 EMDB EMD-54182 associated EM volume AAV2 Heptameric Rep40-dsDNA (ITR) in presence of ATPgS AAV2 Heptameric Rep40-dsDNA (ITR) in presence of ATPgS 0 1 2
Adeno-associated Virus 2 Replication protein 40 in complex with inverted-terminal-repeat DNA and ATP-gamma-S
adeno-associated virus 2 singleParticle particle 0.8 7.8 200.0 NaCl Sodium Chloride 25.0 (HOCH2)*3CNH2 Tris 1.0 C9H15O6P TCEP 1.0 C3H8O3 Glycerol ETHANE 100 295.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.6 2.4 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER EPU 3.8.1 GATAN K3 (6k x 4k) 5760 4092 1.68 39.99 1 cryoSPARC 4.4.1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.63 FSC 0.143 CUT-OFF cryoSPARC 4.4.1 137594 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD cryoSPARC 4.4.1 emd_54182.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 297.0 297.0 297.0 90.0 90.0 90.0 X Y Z -0.10136336 0.26725847 0.00015135118 0.004460852 0.825 0.825 0.825 0.0406 AUTHOR AlphaFold in silico model ISOLDE 1.8 emd_54182_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 297.0 297.0 297.0 90.0 90.0 90.0 X Y Z -0.21863128 0.31512815 0.000110252935 0.025335982 0.825 0.825 0.825 AUTHOR emd_54182_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 297.0 297.0 297.0 90.0 90.0 90.0 X Y Z -0.23750484 0.32592005 0.00011165115 0.025257455 0.825 0.825 0.825 AUTHOR