2025-11-05 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-07-06 2025-09-10 2025-09-10 2025-11-05 Swiss National Science Foundation Switzerland Electron tomogram of resin-embedded, apoptosis-induced HeLa cell expressing Apaf1-GFP Borgeaud AC Ganeva I Klein C Stooss A Ross-Kaschitza D Wu L Riley JS Tait SWG Lemmin T Kaufmann T Kukulski W Apoptosome, Caspase-activation, APOPTOSIS Borgeaud AC Ganeva I Klein C Stooss A Ross-Kaschitza D Wu L Riley JS Tait SWG Lemmin T Kaufmann T Kukulski W Large transient assemblies of Apaf1 constitute the apoptosome in cells. Nat Commun UK 16 9429 9429 2025 41136486 doi:10.1038/s41467-025-64478-9 2041-1723 EMD-54281 other EM volume EMD-54279 other EM volume EMD-54280 other EM volume EMDB EMD-54281 other EM volume EMDB EMD-54279 other EM volume EMDB EMD-54280 other EM volume EMDB EMD-54278 associated EM volume HeLa cell expressing Apaf1-GFP, treated with ABT-737 and QVD HeLa cell expressing Apaf1-GFP, treated with ABT-737 and QVD 0 Homo sapiens tomography cell 7.0 NEGATIVE Uranyl acetate Lowicryl HM20 NITROGEN
Cells grown on sapphire disks, high-pressure frozen and freeze-substituted
Agar Scientific 15 OTHER
The value given for _em_high_pressure_freezing.instrument is Leica HPM100. This is not in a list of allowed values {'EMS-002 RAPID IMMERSION FREEZER', 'LEICA EM HPM100', 'OTHER', 'BAL-TEC HPM 010', 'LEICA EM PACT2', 'LEICA EM PACT'} so OTHER is written into the XML file.
 Leica 293 300 FEI TECNAI F20 OTHER BRIGHT FIELD FIELD EMISSION GUN 200 0.0 0.0
STEM mode on an axial brightfield detector
OTHER 1000.0
ET was done in STEM mode on an axial brightfield detector with a high-tilt tomography holder at 1.102 nm pixel size with a camera length of 200 mm. [NOTE: Electron dose unknown. Value specified here is a dummy]
 1 IMOD 242 NONE emd_54278.map.gz 1 IMAGE STORED AS SIGNED BYTE 1024 1024 112 0 0 -56 1024 1024 112 22568.96 22568.96 2468.48 90.0 90.0 90.0 X Y Z -128.0 127.0 55.486812999999998 11.850663000000001 22.04 22.04 22.039999 AUTHOR Electron tomogram of resin-embedded, apoptosis-induced HeLa cell expressing Apaf1-GFP