Egalitarian-BicD in complex with K10 TLS RNA (Structure B)

2026-03-25 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-07-09 2026-03-25 2026-03-25 2026-03-25 Medical Research Council (MRC, United Kingdom) MC_UP_A025_1011 United Kingdom European Molecular Biology Organization (EMBO) ALTF 197-2021 European Union Egalitarian-BicD in complex with K10 TLS RNA (Structure B) Singh K Chilaeva S McClintock MA Bullock SL Carter AP Complex, Transport, Localization, RNA BINDING PROTEIN Singh K Chilaeva S McClintock MA Bullock SL Carter AP Structures of Egl-BicD reveal how diverse mRNAs are selected for subcellular localization To Be Published 0353 9rvz FULLOVERLAP EMDB EMD-54293 associated EM volume

Egalitarian-BicD in complex with K10 TLS RNA Egalitarian-BicD in complex with K10 TLS RNA 0 1 2 3

Egalitarian-BicD in complex with the transport and localization signal (TLS) of the Drosophila fs(1)K10 mRNA

Drosophila melanogaster Protein bicaudal D Drosophila melanogaster 0.089073547 2 Spodoptera frugiperda LEVO MSSASNNGPSADQSVQDLQMEVERLTRELDQVSSASAQSAQYGLSLLEEKSALQQKCEELETLYDNTRHELDITQEALTK FQTSQKVTNKTGIEQEDALLNESAARETSLNLQIFDLENELKQLRHELERVRNERDRMLQENSDFGRDKSDSEADRLRLK SELKDLKFRETRMLSEYSELEEENISLQKQVSSLRSSQVEFEGAKHEIRRLTEEVELLNQQVDELANLKKIAEKQMEEAL ETLQGEREAKYALKKELDGHLNRESMYHISNLAYSIRSNMEDNASNNSDGEEENLALKRLEADLSTELKSPDGTKCDLFS EIHLNELKKLEKQLESMESEKTHLTANLREAQTSLDKSQNELQNFMSRLALLAAHVDALVQLKKQIDVKEQGKEGGQKKD ELEQQLRALISQYANWFTLSAKEIDGLKTDIAELQKGLNYTDATTTLRNEVTNLKNKLLATEQKSLDLQSDVQTLTHISQ NAGQSLGSARSTLVALSDDLAQLYHLVCTVNGETPTRVLLDHKTDDMSFENDSLTAIQSQFKSDVFIAKPQIVEDLQGLA DSVEIKKYVDTVSDQIKYLKTAVEHTIDMNKHKIRSEGGDALEKVNTEEMEELQEQIVKLKSLLSVKREQIGTLRNVLKS NKQTAEVALTNLKSKYENEKIIVSDTMSKLRNELRLLKEDAATFSSLRAMFAARCEEYVTQVDDLNRQLEAAEEEKKTLN QLLRLAVQQKLALTQRLEEMEMDREMRHVRRPMPAQRGTSGKSSFSTRPSSRNPASSNANPF P16568 Egalitarian, isoform B Drosophila melanogaster 0.113198102 2 Spodoptera frugiperda LEVO MESMEYEMARNMTLLFFLERLLDKGEPRTVHDLSCQFGNKEFTKEMRQIAGGSQSGLKKFLAQYPAIFLVDGDYVQVNAY QHHNADDGGCGGKRDYIQEAKDYFKNKMLQYGAAAEVPVRSLLGHRSQASPQVRHISGQHIKEFTDFLMKHTDTFKVTDD YVMLVGCENMTDLPARDRLHLPQSNIDTRGTQQMLDFFAQCIEVKGPLLVDQLFHLLTTNFPQDQWLRMFKTPGDLSSFL KLFADCFHIQANLVTLLQKPKLSDTHIQQAQAQTREQFNALNNNNSASIRKQEPTPGGGGVGGVSSVQQRLQSPALRTNG HTNNNNGSNGSNNNNNNNNSIACPNFKLNAPVSNVMGGQSQGFGQPKSEPSSGFDSYVPMSELKLENLCENNYPSANTCY GPINNSSQQTQQVQTQQQQQPQHATQNPAEQRLNSVNQTLKQRINTLVIRTLAENLEKDKQSLANQQGGPISPHASPVHS IANSSSNQNAGSAANNANSNSNANPNNANHSPSHSYFVGDTWKIKVLQNTTVIANVKQSVFVTDIILKYAAKNESIVVSL DCEGINLGLKGEITLIEIGTTRGEAFLFDVQSCPAMVTDGGLKTVLEHDQVIKVIHDCRNDAANLYLQFGILLRNVFDTQ AAHAILQYQESGKQVYKAKYISLNSLCEQYNAPCNPIKDQLKQIYRRDQKFWAKRPLTREMMLYAAGDVLVLIHDQLFGN LARQIKPENRALFSELCTEQILMQIKPNEVKIRKKQRKVSTEVSDLKQKLAQTSKSIVLSNREIRLLRYMDLTEDEKERL KGYYKVAKKLEKMESAGNPSKDQSDSEDEQEPNENDAFPSLDSVPSDNSLSGTFSPRFSSEPPSLTESMQMLEEILQNKS MDRIARIDKLEAILTTATSLPCEQIIASNSMQEQLGSSIATTENLQIIREKSKNIKNCNCQGERSVTPIMRTTDKRVVKL VDAESQTLSTGDVVITKIFFQDEHERAKEAALLSNSPAKRVSPTGSENLYFQ Q9W1K4 TLS Drosophila melanogaster 0.018962187999999998 2 UUACACCACUUGAUUGUAUUUUUAAAUUAAUUCUUAAAAACUACAAAUUAAGAUCACUCU singleParticle particle 7.3

25 mM HEPES pH 7.3, 150 mM KCl, 1 mM MgCl2, 1mM DTT, 0.00125% IGEPAL

ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 1.0 3.5 81000.0 GATAN K3 BIOQUANTUM (6k x 4k) 48.0 1 CTFFIND 4.1 PHASE FLIPPING AND AMPLITUDE CORRECTION

Ab-initio model was generated in cryoSPARC

3.2 FSC 0.143 CUT-OFF RELION 5 256835 MAXIMUM LIKELIHOOD cryoSPARC 4

Cryosparc

MAXIMUM LIKELIHOOD RELION 5 emd_54293.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 254.16 254.16 254.16 90.0 90.0 90.0 X Y Z -0.032781206 0.07552943 0.000016625267 0.0015446566 1.059 1.059 1.059 0.01 AUTHOR ::::EMDATABANK.org::::EMD-54293:::: sharpened map AlphaFold in silico model

Predicted models used for all components

emd_54293_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 254.16 254.16 254.16 90.0 90.0 90.0 X Y Z -0.015933348 0.044344157 0.000016622938 0.0010145669 1.059 1.059 1.059 AUTHOR ::::EMDATABANK.org::::EMD-54293:::: unsharpened map emd_54293_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 254.16 254.16 254.16 90.0 90.0 90.0 X Y Z -0.018188482 0.047294058 0.000016835715 0.0014246866 1.059 1.059 1.059 AUTHOR ::::EMDATABANK.org::::EMD-54293:::: emd_54293_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 254.16 254.16 254.16 90.0 90.0 90.0 X Y Z -0.020001423 0.048958752 0.000016414819 0.0014347138 1.059 1.059 1.059 AUTHOR ::::EMDATABANK.org::::EMD-54293::::