2025-12-24 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation_author.identifier_ORCID _em_admin.last_update PDBe PDBe 2025-07-09 2025-12-10 2025-12-10 2025-12-24 Spanish Ministry of Science, Innovation, and Universities PID2021-126746NB-I00 Spain Spanish Ministry of Science, Innovation, and Universities PID2022-137175NB-I00 Spain Other private RTI2018-094368-B-I00 Ancestral Fibrobacteres-Chlorobi-Bacteroidetes Group Chaperonin (AFCB) Double-Ring in Open Conformation Cuellar J Gutierrez-Seijo J Severino R Maestro-Lopez M Sanchez-Pulido L Santiago C Moreno-Paz M Valpuesta JM Parro V Chaperonins, Ancestors, Evolution, Cryoelectron Microscopy, CHAPERONE Severino R Cuellar J Gutierrez-Seijo J Maestro-Lopez M Sanchez-Pulido L Santiago C Moreno-Paz M Valpuesta JM Parro V Ancestral Chaperonins Provide the First Structural Glimpse into Early Multimeric Protein Evolution. Mol.Biol.Evol. US 42 2025 41310978 doi:10.1093/molbev/msaf314 1537-1719 9rwr FULLOVERLAP EMDB EMD-54341 associated EM volume
Ancestral Fibrobacteres-Chlorobi-Bacteroidetes Group Chaperonin (AFCB) Double-Ring in Open Conformation
 Ancestral Fibrobacteres-Chlorobi-Bacteroidetes Group Chaperonin (AFCB) Ancestral Fibrobacteres-Chlorobi-Bacteroidetes Group Chaperonin (AFCB) 0 1 synthetic construct 0.802 Ancestral Fibrobacteres-Chlorobi-Bacteroidetes Group Chaperonin (AFCB) synthetic construct 0.05605314499999999 14 Escherichia coli BL21(DE3) LEVO AKQIKFDTDARNALLRGVDKLADAVKVTLGPKGRNVIIEKKFGAPTITKDGVTVAKEIELEDPFENMGAQMVKEVASKTS DVAGDGTTTATVLAQAIVREGLKNVAAGANPMDLKRGIDKAVEAVVEELKKMAKPVNGKEEIAQVATISANNDPEIGKLI AEAMEKVGKDGVITVEESKSTETTLDVVEGMQFDRGYLSPYFVTDSEKMEAVLENPYILIYDKKISNMKDLLPILEKVAQ SGKPLLIIAEDVEGEALATLVVNKLRGTLKVCAVKAPGFGDRRKAMLEDIAILTGGTVISEETGYKLENATLDYLGRAKR VTIDKDNTTIVDGAGDKEDIKARVNQIKKQIENTTSDYDREKLQERLAKLAGGVAVIKVGAATEVEMKEKKARVEDALHA TRAAVEEGIVPGGGVALIRAAKALENLEGENGDQKTGVKIVRRALEEPLRQIVANAGLEGSVVVNKVKEGKGNFGYNART EEYDLIEAGVIDPAKVTRTALQNAASIAGMLLTTECVITEKP singleParticle particle 7.4 Quantifoil R2/2 COPPER/RHODIUM 300 GLOW DISCHARGE ETHANE FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 0.8 2.2 120000.0 FEI FALCON III (4k x 4k) COUNTING 1 43.0 1 245876 Gctf 3.0 PHASE FLIPPING AND AMPLITUDE CORRECTION
CryoSparc Initial Model
 C1 3.45 FSC 0.143 CUT-OFF cryoSPARC 4.0 44531 MAXIMUM LIKELIHOOD RELION 5.0 cryoSPARC 4.0 MAXIMUM LIKELIHOOD cryoSPARC 4.0 3 RELION 5.0 emd_54341.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 260 260 260 0 0 0 260 260 260 307.788 307.788 307.788 90.0 90.0 90.0 X Y Z -0.0018977025 2.0331275 0.0055305837 0.05603248 1.1838 1.1838 1.1838 0.117 AUTHOR Ancestral Fibrobacteres-Chlorobi-Bacteroidetes Group Chaperonin (AFCB) Double-Ring in Open Conformation AlphaFold in silico model AB INITIO MODEL emd_54341_msk_1.map emd_54341_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 260 260 260 0 0 0 260 260 260 307.788 307.788 307.788 90.0 90.0 90.0 X Y Z -0.78961915 1.760605 0.0016913937 0.12393775 1.1838 1.1838 1.1838 AUTHOR emd_54341_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 260 260 260 0 0 0 260 260 260 307.788 307.788 307.788 90.0 90.0 90.0 X Y Z -0.84546167 1.7828306 0.0017324853 0.124013074 1.1838 1.1838 1.1838 AUTHOR