<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_11_0/emdb.xsd" version="3.0.11.0" emdb_id="EMD-54398">
    <admin>
        <current_status>
            <date>2026-04-08</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <revision_history>
            <revision version="1.0" date="2026-02-25">
                <change_list>
                    <metadata>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </metadata>
                    <image>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </image>
                    <primary_map>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </primary_map>
                </change_list>
            </revision>
        </revision_history>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-07-15</deposition>
            <header_release>2026-02-25</header_release>
            <map_release>2026-02-25</map_release>
            <update>2026-04-08</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>German Research Foundation (DFG)</funding_body>
                <code>1286</code>
                <country>Germany</country>
            </grant_reference>
        </grant_support>
        <title>In situ cryo-electron tomogram of rat hippocampal synapse</title>
        <authors_list>
            <author ORCID="0000-0003-4448-4230">Petrovic A</author>
            <author ORCID="0009-0004-2374-5803">Do TT</author>
            <author ORCID="0000-0001-8874-8109">Siegert A</author>
            <author ORCID="0000-0002-8366-7622">Fernandez-Busandiego R</author>
        </authors_list>
        <keywords>synapse, synaptic vesicle, MEMBRANE PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Do TT</author>
                    <author order="2">Siegert A</author>
                    <author order="3">Domart F</author>
                    <author order="4">Hahn F</author>
                    <author order="5">Zeising C</author>
                    <author order="6">Muth S</author>
                    <author order="7">Pape C</author>
                    <author order="8">Kusch K</author>
                    <author order="9">Dresbach T</author>
                    <author order="10">Rizzoli SO</author>
                    <author order="11">Petrovic A</author>
                    <author order="12">Fernandez-Busnadiego R</author>
                    <title>A correlative workflow for synaptic imaging by cryo-electron tomography.</title>
                    <journal_abbreviation>Structure</journal_abbreviation>
                    <country>UK</country>
                    <year>2026</year>
                    <external_references type="PUBMED">41881019</external_references>
                    <external_references type="DOI">doi:10.1016/j.str.2026.02.017</external_references>
                    <external_references type="ISSN">0969-2126</external_references>
                    <external_references type="CSD">2005</external_references>
                    <external_references type="ASTM">STRUE6</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-54384</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-54384</accession_id>
                <content_type>other EM volume</content_type>
            </db_reference>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-54398</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>primary hippocampal neurons</name>
        <supramolecule_list>
            <cell_supramolecule supramolecule_id="1">
                <name>primary hippocampal neurons</name>
                <parent>0</parent>
                <details>synapse</details>
                <natural_source database="NCBI">
                    <organism ncbi="10116">Rattus norvegicus</organism>
                </natural_source>
            </cell_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>cell</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>7.2</ph>
                    </buffer>
                    <grid>
                        <model>Quantifoil R2/2</model>
                        <material>GOLD</material>
                        <mesh>200</mesh>
                        <support_film film_type_id="1">
                            <film_material>SILICON DIOXIDE</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE-PROPANE</cryogen_name>
                        <instrument>HOMEMADE PLUNGER</instrument>
                    </vitrification>
                    <details>FIB-milled lamella of rat primary hippocampal neurons</details>
                    <cryo_protectant>5% glycerol</cryo_protectant>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage>30</voltage>
                            <current>0.05</current>
                            <duration>3600</duration>
                            <temperature units="K">83</temperature>
                            <initial_thickness>5000</initial_thickness>
                            <final_thickness>150</final_thickness>
                            <details>The value given for _em_focused_ion_beam.instrument is Aquilos 2. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file.</details>
                        </focused_ion_beam>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">50.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">3.0</nominal_defocus_min>
                    <calibrated_defocus_min units="µm">3.0</calibrated_defocus_min>
                    <nominal_defocus_max units="µm">5.0</nominal_defocus_max>
                    <calibrated_defocus_max units="µm">5.0</calibrated_defocus_max>
                    <nominal_magnification>64000.0</nominal_magnification>
                    <calibrated_magnification>64000.0</calibrated_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <basic/>
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>TFS Selectris</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON IV (4k x 4k)</film_or_detector_model>
                            <digitization_details>
                                <dimensions>
                                    <width units="pixel">4096</width>
                                    <height units="pixel">4096</height>
                                </dimensions>
                            </digitization_details>
                            <average_electron_dose_per_image units="e/Å^2">3.24</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <software_list>
                        <software>
                            <name>IMOD</name>
                            <version>4.12</version>
                        </software>
                    </software_list>
                    <number_images_used>37</number_images_used>
                </final_reconstruction>
                <ctf_correction>
                    <type>NONE</type>
                </ctf_correction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="2097153">
        <file>emd_54398.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>1024</col>
            <row>1024</row>
            <sec>500</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>1024</x>
            <y>1024</y>
            <z>500</z>
        </spacing>
        <cell>
            <a units="Å">7741.44</a>
            <b units="Å">7741.44</b>
            <c units="Å">3780.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-187.674029999999988</minimum>
            <maximum>84.470470000000006</maximum>
            <average>1.0111912</average>
            <std>9.7181015</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">7.56</x>
            <y units="Å">7.56</y>
            <z units="Å">7.56</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-54398::::</label>
        <annotation_details>synaptic tomogram from rat primary neurons</annotation_details>
    </map>
</emd>
