2026-01-14 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update PDBe PDBe 2025-08-15 2025-12-24 2025-12-24 2026-01-14 German Research Foundation (DFG) SCHU3196/1-1 Germany Structure of RBR E2 variant binding to CUL5-RBX2 bound ARIH2 Schulman BA Du J Complex, Ligase Du J Andree GA Horn-Ghetko D Stier L Singh J Kostrhon S Kiss L Mann M Sidhu SS Schulman BA E2 variants for probing E3 ubiquitin ligase activities. Proc.Natl.Acad.Sci.USA US 123 e2524899122 e2524899122 2026 41481455 doi:10.1073/pnas.2524899122 1091-6490 0040 PNASA6 EMD-54892 consensus EM volume EMD-54893 focused EM volume 9sdy FULLOVERLAP EMDB EMD-54794 associated EM volume
Structure of Neddylated CUL5 C-terminal region-RBX2-ARIH2-E2V
 EMDB EMD-54892 consensus EM volume EMDB EMD-54893 focused EM volume NEDD8-CUL5-RBX2-ARIH2-E2V NEDD8-CUL5-RBX2-ARIH2-E2V 0 1 2 3 4 Homo sapiens 200 L3A2-1 synthetic construct 0.017854474999999998 1 Escherichia coli LEVO MVAASSRLMKELEEIRKAGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNI DEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFAKNAEEFTKKYGEKRPVD RING-box protein 2 Homo sapiens 0.012697436 1 Trichoplusia ni LEVO MADVEDGEETCALASHSGSSGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGEC NHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK Q9UBF6 2.3.2.27 Cullin-5 Homo sapiens 0.091085297 1 Trichoplusia ni LEVO MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARV LSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAM KLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKE EEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIIS AGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQP ESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWL REVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEE FYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAF PKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAII QIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA Q93034 E3 ubiquitin-protein ligase ARIH2 Homo sapiens 0.05789384399999999 1 Escherichia coli LEVO MSVDMNSQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEGALNEHMTSL ASVLKVSHSVAKLILVNFHWQVSEILDRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQF CRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQ EPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKH DFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNN LGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAE QRRRTLLKDFHDT O95376 2.3.2.31 ZINC ION 6.5409e-05 7 ZN singleParticle particle 7.5 ETHANE-PROPANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.6 NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) 60.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 8ONI 3.06 FSC 0.143 CUT-OFF cryoSPARC 191502 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_54794.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 367.71838 367.71838 367.71838 90.0 90.0 90.0 X Y Z -0.037036724 0.74711055 0.0021889557 0.012030967 0.8512 0.8512 0.8512 0.14 AUTHOR emd_54794_msk_1.map emd_54794_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 367.71838 367.71838 367.71838 90.0 90.0 90.0 X Y Z -0.0019315879 3.9332268 0.0022897483 0.040497936 0.8512 0.8512 0.8512 AUTHOR emd_54794_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 367.71838 367.71838 367.71838 90.0 90.0 90.0 X Y Z -0.51593673 0.74711055 -0.0004924625 0.008522773 0.8512 0.8512 0.8512 AUTHOR emd_54794_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 367.71838 367.71838 367.71838 90.0 90.0 90.0 X Y Z -0.27915102 0.5406152 -0.00049524993 0.009168105 0.8512 0.8512 0.8512 AUTHOR emd_54794_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 367.71838 367.71838 367.71838 90.0 90.0 90.0 X Y Z -0.3380695 0.66399854 -0.000064237545 0.028220745 0.8512 0.8512 0.8512 AUTHOR emd_54794_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 367.71838 367.71838 367.71838 90.0 90.0 90.0 X Y Z -0.35817254 0.62379247 -0.000065701715 0.027991157 0.8512 0.8512 0.8512 AUTHOR