2025-10-08 REL PDBe PDBe PDBe 2025-08-26 2025-10-01 2025-10-01 2025-10-08 Not funded Focus map of ternary PROTAC-mediated complex of BRD4-BD1/CRBN/DDB1 and JQ1-AcQ bifunctional degrader Peter D Fischer G Arce Solano S Kessler D Cryo-EM DDB1 (DNA Damage-Binding Protein 1) CRBN (Cereblon) BRD4 (bromodomain containing 4) Protein degradation cyclimid PROTACs, LIGASE Zhu Q Fischer G Cheng SS Payne NC Peter D Mody AC Arce-Solano S Shen D Lin Z Mazitschek R Kessler D Woo CM Enzyme-Activated Sugar-Coated Bifunctional Degraders. J.Am.Chem.Soc. US 147 34672 34680 2025 40937862 doi:10.1021/jacs.5c09843 1520-5126 JACSAT EMDB EMD-54895 associated EM volume PROTAC-mediated complex of DDB1 (BPB deletion), CRBN and BRD4 BD1 domain PROTAC-mediated complex of DDB1 (BPB deletion), CRBN and BRD4 BD1 domain 0 1 2 3 Homo sapiens 0.015 Ligase sub-complex consisting of DDB1 (BPB deletion) and CRBN 1 1 2 Homo sapiens degradation target BRD4 (BD1 domain) 1 3 Homo sapiens singleParticle particle 0.3 7.5 20.0 HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 100.0 NaCl sodium chloride 0.25 TCEP Tris(2-carboxyethyl)phosphine ETHANE 100 277.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.8 2.0 165000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris X 10 FEI FALCON IV (4k x 4k) 3 38923 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.85 FSC 0.143 CUT-OFF cryoSPARC 158212 PROJECTION MATCHING PROJECTION MATCHING emd_54895.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 287.616 287.616 287.616 90.0 90.0 90.0 X Y Z -0.11268924 0.20857054 -0.00006813784 0.0026077183 0.749 0.749 0.749 0.04 AUTHOR focus map for deposition D_1292150008; emd_54895_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 287.616 287.616 287.616 90.0 90.0 90.0 X Y Z -0.18794487 0.2719154 -0.000040475275 0.022892289 0.749 0.749 0.749 AUTHOR emd_54895_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 287.616 287.616 287.616 90.0 90.0 90.0 X Y Z -0.18850651 0.29980874 -0.000039819297 0.022782996 0.749 0.749 0.749 AUTHOR