2026-02-04 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-09-03 2026-02-04 2026-02-04 2026-02-04 Swiss National Science Foundation 200021_200628 Switzerland European Research Council (ERC) 101118656 European Union Cryo-EM structure of the transient receptor potential melastatin 4 (TRPM4) channel prepared using the cryoWriter automated grid preparation system with spiral-pattern writing Chinmaya KV Ekundayo B Di Fabrizio M Mohammed I Radecke J Stahlberg H Kube M automated grid preparation, TRPM4, cryo-EM, cryoWriter, TRANSPORT PROTEIN Chinmaya KV Ekundayo B Di Fabrizio M Mohammed I Radecke J Stahlberg H Kube M CryoWriter: A Robotic Solution for Improved Cryo-EM Grid Preparation To Be Published 0353 EMDB EMD-54984 associated EM volume Transient receptor potential (TRP) melastatin 4 (TRPM4) Transient receptor potential (TRP) melastatin 4 (TRPM4) 0 1 Homo sapiens 536 Transient receptor potential (TRP) melastatin 4 (TRPM4) Homo sapiens Homo sapiens DEXTRO RSFHLEASLM DALLNDRPEF VRLLISHGLS LGHFLTPMRL AQLYSAAPSN SLIRNLLDQA SHSAGTKAPA LKGGAAELRP PDVGHVLRML LGKMCAPRYP SGGAWDPHPG QGFGESMYLL SDKATSPLSL DAGLGQAPWS DLLLWALLLN RAQMAMYFWE MGSNAVSSAL GACLLLRVMA RLEPDAEEAA RRKDLAFKFE GMGVDLFGEC YRSSEVRAAR LLLRRCPLWG DATCLQLAMQ ADARAFFAQD GVQSLLTQKW WGDMASTTPI WALVLAFFCP PLIYTRLITF RKSEEEPTRE ELEFDMDSVI NGEGPVGTAD PAEKTPLGVP RQSGRPGCCG GRCGGRRCLR RWFHFWGAPV TIFMGNVVSY LLFLLLFSRV LLVDFQPAPP GSLELLLYFW AFTLLCEELR QGLSGGGGSL ASGGPGPGHA SLSQRLRLYL ADSWNQCDLV ALTCFLLGVG CRLTPGLYHL GRTVLCIDFM VFTVRLLHIF TVNKQLGPKI VIVSKMMKDV FFFLFFLGVW LVAYGVATEG LLRPRDSDFP SILRRVFYRP YLQIFGQIPQ EDMDVALMEH SNCSSEPGFW AHPPGAQAGT CVSQYANWLV VLLLVIFLLV ANILLVNLLI AMFSYTFGKV QGNSDLYWKA QRYRLIREFH SRPALAPPFI VISHLRLLLR QLCRRPRSPQ PSSPALEHFR VYLSKEAERK LLTWESVHKE NFLLARARDK RESDSERLKR TSQKVDLALK QLGHIREY singleParticle particle 6 8.0 Quantifoil R1.2/1.3 COPPER 300 CARBON HOLEY GLOW DISCHARGE AIR ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 2.0 165000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON IV (4k x 4k) 50.0 1 695101 cryoSPARC v4 PHASE FLIPPING AND AMPLITUDE CORRECTION C4 3.01 FSC 0.143 CUT-OFF cryoSPARC v4 69878 PROJECTION MATCHING cryoSPARC v4 MAXIMUM LIKELIHOOD cryoSPARC v4 1 cryoSPARC v4 emd_54984.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 374.79202 374.79202 374.79202 90.0 90.0 90.0 X Y Z -0.7395916 1.0621921 0.0019524283 0.01947225 0.8518 0.8518 0.8518 0.181 AUTHOR emd_54984_msk_1.map emd_54984_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 374.79202 374.79202 374.79202 90.0 90.0 90.0 X Y Z -1.1002978 1.0314115 0.0012617675 0.055648133 0.8518 0.8518 0.8518 AUTHOR emd_54984_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 374.79202 374.79202 374.79202 90.0 90.0 90.0 X Y Z -0.93838817 0.9643179 0.0012592161 0.055813964 0.8518 0.8518 0.8518 AUTHOR