Reduced bovine complex I in lipid nanodisc, NADH-active-DDM

2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-09-08 2026-03-18 2026-03-18 2026-03-18 Medical Research Council (MRC, United Kingdom) MC_UU_00015/2 United Kingdom Medical Research Council (MRC, United Kingdom) MC_UU_00028/1 United Kingdom Diamond Light Source BI22238-37 United Kingdom Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Chung I Hirst J Mitochondrial complex I, Respiratory complex I, NADH:ubiquinone oxidoreductase, Ubiquinone, Nanodisc, NADH, ELECTRON TRANSPORT Chung I Pereira CS Wright JJ Arantes GM Hirst J Post-catalysis structures of mitochondrial complex I with ubiquinol-10 bound in the active site. Nat Commun UK 2026 41786699 doi:10.1038/s41467-026-70030-0 2041-1723 9smh FULLOVERLAP EMDB EMD-55032 associated EM volume

Mitochondrial respiratory complex I Mitochondrial respiratory complex I 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Bos taurus heart Mitochondria 1 NADH-ubiquinone oxidoreductase chain 3 Bos taurus Heart 0.013086531 1 LEVO (FME)NLMLALLTNFTLATLLVIIAFWLPQLNVYSEKTSPYECGFDPMGSARLPFSMKFFLVAITFLLFDLEIALLLPLP WASQTANLNTMLTMALFLIILLAVSLAYEWTQKGLEWTE Q7JAS9 7.1.1.2 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Bos taurus Heart 0.023817916 1 LEVO MAALAALRLLHPILAVRSGVGAALQVRGVHSSMAADSPSSTQPAVSQARAVVPKPAALPSSRGEYVVAKLDDLINWARRS SLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVF(WYK)ASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSC ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQKKIKREKRLRIWYRR P42026 7.1.1.2 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Bos taurus Heart 0.030323444999999997 1 LEVO MAAAVAAAARGCWQRLVGSAAPARVAGRPSVLLLPVRRESSAADTRPTVRPRNDVAHKQLSAFGEYVAEILPKYVQQVQV SCFNELEICIHPDGVIPVLTFLRDHSNAQFKSLADLTAVDIPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESSVP VYKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSP WEAFPAYRQPPESLKLEAGDTKPEAK P23709 7.1.1.2 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Bos taurus Heart 0.052678547

Harbours a naturally occurring mutation Q129R (Q162R) in the Cambridge UK population

1 LEVO MAALRALCRLRGAAAQVLRPGAGVRLPIQPSRGARQWQPDVEWAEQYGGAVMYPTKETAHWKPPPWNDVDPPKDTLVSNL TLNFGPQHPAAHGVLRLVMELSGEMVRKCDPHIGLLH(2MR)GTEKLIEYKTYLQALPYFDRLDYVSMMCNEQAYSLAVE KLLNIRPPPRAQWIRVLFGEITRLLNHIMAVTTHALDIGAMTPFFWMFEEREKMFEFYERVSGARMHAAYVRPGGVHQDL PLGLMDDIYEFSKNFSLRIDELEEMLTNNRIWRNRTVDIGIVTAEDALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEF DVPIGSRGDCYDRYLCRVEEMRQSIRIISQCLNKMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGAT YTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR P17694 7.1.1.2 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Bos taurus Heart 0.027341495999999996 1 LEVO MFLSAALRARAAGLAAHWGKHIRNLHKTAVQNGAGGALFVHRDTPENNPETPFDFTPENYKRIEAIVKNYPEGHKAAAVL PVLDLAQRQNGWLPISAMNKVAEILQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCMLRNSDSILEAIQKKLGIKV GETTPDKLFTLIEVECLGACVNAPMVQINDNYYEDLTPKDIEEIIDELKAGKIPKPGPRSGRFSCEPAGGLTSLTEPPKG PGFGVQAGL P04394 1.6.5.3 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Bos taurus Heart 0.050718777 1 LEVO MLAARRLLGGSLPARVSVRFSGDTTAPKKTSFGSLKDEDRIFTNLYGRHDWRLKGAQSRGDWYKTKEILLKGPDWILGEV KTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIIRHDPHKLVEGCLVGGRAMGARAAYIYIRG EFYNEASNLQVAIREAYEAGLIGKNACGSGYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPT TVANVETVAVSPTICRRGGAWFASFGRERNSGTKLFNISGHVNNPCTVEEEMSVPLKELIEKHAGGVTGGWDNLLAVIPG GSSTPLIPKSVCETVLMDFDALIQAQTGLGTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFV RGDARPAEIDSLWEISKQIEGHTICALGDGAAWPVQGLIRHFRPELEERMQQFAQQHQARQAAF P25708 1.6.5.3 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Bos taurus Heart 0.07953221899999999 1 LEVO MLRIPVRKALVGLSKSSKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLV EIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAV EDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTA RPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWE DALSRVAGMLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVV LLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILGSSA LQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIQKNPPKMLFLLGADGGCITRQDL PKDCFIVYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLD QVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAHA VEEPSIC P15690 7.1.1.2 NADH-ubiquinone oxidoreductase chain 1 Bos taurus Heart 0.035716785 1 LEVO (FME)FMINILMLIIPILLAVAFLTLVERKVLGYMQLRKGPNVVGPYGLLQPIADAIKLFIKEPLRPATSSASMFILAPI MALGLALTMWIPLPMPYPLINMNLGVLFMLAMSSLAVYSILWSGWASNSKYALIGALRAVAQTISYEVTLAIILLSVLLM SGSFTLSTLITTQEQMWLILPAWPLAMMWFISTLAETNRAPFDLTEGESELVSGFNVEYAAGPFALFFMAEYANIIMMNI FTAILFLGTSHNPHMPELYTINFTIKSLLLTMSFLWIRASYPRFRYDQLMHLLWKNFLPLTLALCMWHVSLPILTSGIPP QT P03887 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Bos taurus Heart 0.0239264 1 LEVO MRCLTMPMLLRALAQAQAARAGHASVRGLHSSAVAATYKYVNLREPSMDMKSVTDRAAQTLLWTELIRGLGMTLSYLFRE PATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAVCPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQE ACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR P42028 7.1.1.2 NADH-ubiquinone oxidoreductase chain 6 Bos taurus Heart 0.019110488000000002 1 LEVO (FME)MLYIVFILSVIFVMGFVGFSSKPSPIYGGLGLIVSGGVGCGIVLNFGGSFLGLMVFLIYLGGMMVVFGYTTAMAT EQYPEIWLSNKAVLGAFVTGLLMEFFMVYYVLKDKEVEVVFEFNGLGDWVIYDTGDSGFFSEEAMGIAALYSYGTWLVIV TGWSLLIGVVVIMEITRGN P03924 7.1.1.2 NADH-ubiquinone oxidoreductase chain 4L Bos taurus Heart 0.010828194999999999 1 LEVO (FME)SMVYMNIMMAFTVSLVGLLMYRSHLMSSLLCLEGMMLSLFVMAALTILNSHFTLASMMPIILLVFAACEAALGLS LLVMVSNTYGTDYVQNLNLLQC P03902 7.1.1.2 NADH-ubiquinone oxidoreductase chain 5 Bos taurus Heart 0.06835593 1 LEVO (FME)NMFSSLSLVTLLLLTMPIMMMSFNTYKPSNYPLYVKTAISYAFITSMIPTMMFIHSGQELIISNWHWLTIQTLKL SLSFKMDYFSMMFIPVALFVTWSIMEFSMWYMYSDPNINKFFKYLLLFLITMLILVTANNLFQLFIGWEGVGIMSFLLIG WWYGRADANTAALQAILYNRIGDIGFILAMAWFLTNLNTWDLQQIFMLNPSDSNMPLIGLALAATGKSAQFGLHPWLPSA MEGPTPVSALLHSSTMVVAGIFLLIRFYPLTENNKYIQSITLCLGAITTLFTAMCALTQNDIKKIIAFSTSSQLGLMMVT IGINQPYLAFLHICTHAFFKAMLFMCSGSIIHSLNDEQDIRKMGGLFKAMPFTTTALIVGSLALTGMPFLTGFYSKDLII EAANTSYTNAWALLMTLIATSFTAIYSTRIIFFALLGQPRFPTLVNINENNPLLINSIKRLLIGSLFAGYIISNNIPPTT IPQMTMPYYLKTTALIVTILGFILALEISNMTKNLKYHYPSNAFKFSTLLGYFPTIMHRLAPYMNLSMSQKSASSLLDLI WLEAILPKTISLAQMKASTLVTNQKGLIKLYFLSFLITILISMILFNFHE P03920 7.1.1.2 NADH-ubiquinone oxidoreductase chain 4 Bos taurus Heart 0.052158758 1 LEVO (FME)LKYIIPTIMLMPLTWLSKNNMIWVNSTAHSLLISFTSLLLMNQFGDNSLNFSLLFFSDSLSTPLLILTMWLLPLM LMASQHHLSKENLTRKKLFITMLISLQLFLIMTFTAMELILFYILFEATLVPTLIIITRWGNQTERLNAGLYFLFYTLAG SLPLLVALIYIQNTVGSLNFLMLQYWVQPVHNSWSNVFMWLACMMAFMVKMPLYGLHLWLPKAHVEAPIAGSMVLAAVLL KLGGYGMLRITLILNPMTDFMAYPFIMLSLWGMIMTSSICLRQTDLKSLIAYSSVSHMALVIVAILIQTPWSYMGATALM IAHGLTSSMLFCLANSNYERIHSRTMILARGLQTLLPLMATWWLLASLTNLALPPTINLIGELFVVMSTFSWSNITIILM GVNMVITALYSLYMLIMTQRGKYTYHINNISPSFTRENALMSLHILPLLLLTLNPKIILGPLY P03910 7.1.1.2 NADH-ubiquinone oxidoreductase chain 2 Bos taurus Heart 0.039302940999999994 1 LEVO (FME)NPIIFIIILLTIMLGTIIVMISSHWLLVWIGFEMNMLAIIPIMMKNHNPRATEASTKYFLTQSTASMLLMMAVII NLMFSGQWTVMKLFNPMASMLMTMALAMKLGMAPFHFWVPEVTQGIPLSSGLILLTWQKLAPMSVLYQIFPSINLNLILT LSVLSILIGGWGGLNQTQLRKIMAYSSIAHMGWMTAVLPYNPTMTLLNLIIYIIMTSTMFTMFMANSTTTTLSLSHTWNK TPIMTVLILATLLSMGGLPPLSGFMPKWMIIQEMTKNNSIILPTFMAITALLNLYFYMRLTYSTTLTMFPSTNNMKMKWQ FPLMKKMTFLPTMVVLSTMMLPLTPMLSVLE P03892 7.1.1.2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Bos taurus Heart 0.03933098399999999

Contains a naturally occurring mutation N255K (N278K) present in the Cambridge UK population

1 LEVO MALRLLRLVPPRVGGIHTSVQFKLQYGPLAYILGEKATKKMTEKSKLITVDGNICSGKSKLAKEIAEKLGLKHFPEAGIH YVDSTTGDGKPLPVQFSGNCSLEKFYDDPKSNDGNSYRLQAWLYASRLLQYADALEHLLSTGQGVVLERSIYSDFVFLEA MYRQGFIRKQCVDHYNQVKKVTICEYLPPHVVVYVDVPVPEVQSRIQKKGNPHEMKITSAYLQDIENAYKGTFLPEMSEK CEVLQYSAWEAQDAEKVVEDIEYLKYDKGPWLDQNDRKLHKLRMLVQDKLEVLNYTSIPVFLPEVTVGAHQSDQVFQEFT ELPGRKYRAGYNEDVGDKWIWLK P34942 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Bos taurus Heart 0.042913429999999995 1 LEVO MAAAVHPRVVRVLPMSRSSVPALAASVFHSPPQRQLHHAVIPHGKGGRSSVSGIVATVFGATGFLGRYVVNHLGRMGSQV IVPHRCEPYDTMHLRPMGDLGQIIFMDWNGRDKDSIRRAVEHSSVVINLVGREWETQNFDFEDVFVKIPQAIAQVSKEAG VEKFIHISHLNADIKSSSKYLRSKAVGEKEVRETFPEATIIKPAEIFGREDRFLNYFANIRWFGGVPLISLGKKTVKQPV YIVDVTKGIINAIKDPDARGKTFAFVGPSRYLLFDLVQYVFAVAHRPFLPYPLPHFAYRWIGRLFEISPFEPWTTRDKVE RIHTTDKILPHLPGLEDLGVEATPLELKAIEVLRRHRTYRWLSSEIEDVQPAKTIPTSGP P34943 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Bos taurus Heart 0.019841555 1 LEVO MAAVSMSVALRQALWGRRVATVAAVSVSKVSTRSLSTSTWRLAQDQTRDTQLITVDEKLDITTITGVPEEHIKTRKARIF VPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTADPLSNLVLTFSTKEDAVAFAEKNGWSYDVEERKVPKPKSKSY GANFSWNKRTRVSTK Q02375 NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Bos taurus Heart 0.013433193999999999 1 LEVO MAAVLTFLRFLGRGGAVTRGLPGGARCFGVRTSPTGEKVTHTGQVYDDGDYRKVRFVGRQKEVNENFAIDLIAEQPVSQV GSRVISCDGGGGALGHPRVYINLDKETKTGTCGYCGLQFRQQHH P23934 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Bos taurus Heart 0.011097824 1 LEVO MAAAAAIRGVRGKLGLREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQEKNVSLN NFSADQVTRALENVLSSKA Q02370 Acyl carrier protein, mitochondrial Bos taurus Heart 0.01742134 2 LEVO MAVRVLCACVRRLPTAFAPLPRLPTLAAARPLSTTLFAAETRTRPGAPLPALVLAQVPGRVTQLCRQYSDAPPLTLEGIK DRVLYVLKLYDKIDPEKLSVNSHFMKDLGLDSLDQVEIIMAMEDEFGFEIPDIDAEKLMCPQEIVDYIADKKDVYE P52505 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Bos taurus Heart 0.013334608 1 LEVO MAGLLKKTTGLVGLAVCETPHERLKILYTKILDVLGHIPKNAAYRKYTEQITNEKLSMVKAEPDVKKLEERLQGGQIEEV ILQAENELSLARKMIQWKPWEPLVEEPPASQWKWPI P23935 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Bos taurus Heart 0.015083543999999999 1 LEVO MAASGLRQAAVAASTSVKPIFSRDMNEAKRRVRELYRAWYREVPNTVHLFQLDISVKQGRDKVREMFKKNAHITDPRVVD LLVIKGKMELEETIKVWKQRTHVMRFFHETEAPRPKDFLSKFYVGHDP Q02366 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Bos taurus Heart 0.020124133000000002 1 LEVO MPGIVELPSLEDLKVQEVKVSSSVLKAAAHHYGAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNQCALEFFRQIKRHCAE PFTEYWTCIDYSGLQLFRRCRKQQAQFDECVLDKLGWVRPDLGDLSKVTKVKTDRPLPENPYHSRARPEPNPEVEGDLKP ARHGSRLFFWTM P42029 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 Bos taurus Heart 0.014666862 1 LEVO (ACE)AKTVLRQYWDIPEGTECHRKTYATTSIGGAAGLVVSAYSVALKTPTSFLEGVARTGRYTFTAAAIGAIFGLTSCI SAQVREKPDDPLNYLIGGCAGGLILGARTRSYGIGAAACAYMGLTAALVKMGQLEGWQVFAEPKV Q8HXG6 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Bos taurus Heart 0.016694395 1 LEVO MAASKVKQDMPPVGGYGPIDYKRNLPRRGLSGYSMFAVGIGALLFGYWSMMKWNRERRRLQIEDFEARIALMPLLQAEKD RRVLQMLRENLEEEATVMKDVPGWKVGESVFHTTRWVTPMMGELYGLRASEEVLSATYGFIWYT Q95KV7 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Bos taurus Heart 0.008117445 1 LEVO MWFEVLPGIAVMGVCLFIPGMATARIHRFSNGGKEKRVAHYPYQWYLMERDRRVSGVNRSYVSKGLENID Q02377 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 Bos taurus Heart 0.00935785 1 LEVO MAERVAAFLKNVWAKEPVLVASFAIAGLAVILPTLSPYTKYSLMINRATPYNYPVPLRDDGNMPDVPSHPQDPQGPSLEW LKRL Q02371 NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial Bos taurus Heart 0.008796210999999998 1 LEVO MAPSALLRPFWKLLAPARFPSVSSSRSKFYIQEPPHGSPNWLKVGLTLGTSVFLWIYLIKQHNEDVLEYKRRNGLE Q02376 NADH dehydrogenase [ubiquinone] 1 subunit C2 Bos taurus Heart 0.014143332 1 LEVO (ACE)MMTGRQGRATFQFLPDEARSLPPPKLTDPRLAFVGFLGYCSGLIDNAIRRRPVLLAGLHRQLLYITSFVFVGYYL LKRQDYMYAVRDHDMFSYIKSHPEDFPEKDKKTYGEVFEEFHPVR Q02827 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 Bos taurus Heart 0.012694671 1 LEVO MPFFDVQKRLGVDLDRWMTIQSAEQPHKIPSRCHAFEKEWIECAHGIGSIRAEKECKIEFEDFRECLLRQKTMKRLHAIR RQREKLIKEGKYTPPPHHSGQEEPRS Q02379 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 Bos taurus Heart 0.006978148 1 LEVO MNLLQVVRDHWVHVLVPMGFVFGYYLDRKNDEKLTAFRNKSLLYKRELKPNEEVTWK Q02378 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial Bos taurus Heart 0.017594944999999997 1 LEVO MAARMLGLCGRRLLAVAATRGLPAARVRWESSSSRAVIAPSTLAGKRPSEPTLRWQEDPEPEDENLYEKNPDSHGYDKDP AVDIWNMRVVFFFGFSIVLVLGSTFVAYLPDYRMQEWARREAERLVKYREAHGLPIMESNCFDPSKIQLPEDED Q8HXG5 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial Bos taurus Heart 0.021624134999999996 1 LEVO MAAMSLLHRASVSAVAALSGRRLGTRLGFGGFLTRDFPKTVAPVRHSGDHGKRLFIIKPSGFYDKRFLKLLRFYILLTGI PVAIGITLINVFIGEAELAEIPEGYVPEHWEYFKHPISRWIARTFFDGPEKNYERTMAILQIEAEKAELRLKELEVRRLM RARGDGPWFHYPTIDKELIDHSPKATPDN Q02380 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Bos taurus Heart 0.015444929 1 LEVO (ACE)SGYTPEEKLRLQQLRELRRRWLKDQELSPREPVLPPQRVSPVERFWNKFLQDGALWKNVIYKTYRHSIFAFTHVL IPVWIIHYYLKYHVTTKPYTIVEKKPRIFPGDTILETGEVIPPMKEFPDQHH Q02367 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial Bos taurus Heart 0.012298858 1 LEVO MAGMSALKRLAPFAHVGGHLFRGRCARAAGARGVRRAGGGAHIEPRYRQFPQLTRSQVIQAEFFSATMWFWILWRFWHDS DAVLGHFPYPDPSQWTDEELGIPPDDED Q02374 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 Bos taurus Heart 0.011160705 1 LEVO MAHGHGHEHGPSKMELPDYKQWKIEGTPLETVQEKLAARGLRDPWGRNEAWRYMGGFANNVSFVGALLKGFKWGFAAFVV AIGAEYYLESQKKDKKHH Q02365 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Bos taurus Heart 0.021678458999999997 1 LEVO MAAARAGVLGVRWLQKAARNVVPLGARTASHITKDMLPGPYPKTPEERAAAAKKYNMRVEDYEPYPDDGTGYGDYPKLPD RSQQERDPWYDWDHPDLRLNWGEPMHWDLDMYIRNRVDTSPTPVNWNLMCKHLFGFVAFMLFMFWVGETYPAYQPVGPKQ YPYNNLYLERGGDPNKEPEPVVHYEI Q02372 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Bos taurus Heart 0.01520626 1 LEVO MSFPKYEASRLSSLPTTLDPAEYDISSETRKAQAERLAIRSRLKREYQLQYYDPSRRGVIEDPALVRWTYARSANIYPNF RPNTKTSLLGALFGIGPLVFWYYVFKTDRDRKEKLIQEGKLDRTFNISY P48305 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Bos taurus Heart 0.021827889 1 LEVO MAFLSSGAYLTHQQKVLRLYKRALRHLESWCIHRDKYRYFACLLRARFDEHKNEKDMVKATQLLREAEEEFWHGQHPQPY IFPESPGGTSYERYECYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPVGGPRTEALPPARKQ GDLPPLWWHIVTRPRERPM Q02369 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Bos taurus Heart 0.016428846 1 LEVO MGAHLARRYLGDASVEPDPLRMPTFPPDYGFPERKEREMVATQQEMNDAQLVLQQRDYCAHYLIRFLKCKRDSFPNFLAC KHERHDWDYCEHLDYVKRMKEFERERRLLQRKKRREQREADMAKGLGPGEVAPEVAL Q02368 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 Bos taurus Heart 0.0210009 1 LEVO MPDSWDKDVYPEPPRRTPAPSPQTSLPNPITYLTKAFDLLVDRPVTLVREFIERQHAKNKYYYYHREFRRVPDITECQEK DVLCMFEAEMQWRRDYKVDQEIVNIIQERLKACQQREGESHRQNCAKELEQFTQVVKAYQDRYHDLGAHYSARKCLAKQK QRMLAERKAAKEAAAA Q02373 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Bos taurus Heart 0.017115508 1 LEVO MELLQVLKRGLQQVSGHGGLRGYLRVLFRANDVRVGTLVGEDKYGNKYYEDNKQFFGRHRWVIYTTEMNGKNTFWDVDGS MVPPEWHRWLHCMTDDPPTVKPPTARKFIWTNHKFNLSGTPQQYVPYSTTRKKIQEWVPPSTPYK O97725 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Bos taurus Heart 0.012590424999999999 1 LEVO (ACE)ASATRFIQWLRNWASGRDLQAKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRREAMPPSIVMSSQKVL VAGKPAESSAVAASEKKAVSPAPPIKRWELSQDEPYL Q05752 NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial Bos taurus Heart 0.011874492 1 LEVO MAASLLLRQGRAGALKTVLLEAGVFRGVAPAVSLSAESGKNEKGLPPNPKKQSPPKKPVSAAPTEPFDNTTYKNLQHHDY STYTFLDLNLDLSKFRMPQPSSGRESPRH P25712 1,2-Distearoyl-sn-glycerophosphoethanolamine 0.000748065 17 3PE 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE 0.0007901449999999999 11 PC1 IRON/SULFUR CLUSTER 0.00035163999999999996 6 SF4 FE2/S2 (INORGANIC) CLUSTER 0.00017581999999999998 2 FES 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE 0.000665441 1 NAI FLAVIN MONONUCLEOTIDE 0.00045634399999999997 1 FMN POTASSIUM ION 3.9098e-05 1 K DODECYL-BETA-D-MALTOSIDE 0.000510615 1 LMT CARDIOLIPIN 0.0014640429999999997 7 CDL 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE 0.000507181 1 DGT MAGNESIUM ION 2.4305e-05 1 MG NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE 0.000745421 1 NDP ZINC ION 6.5409e-05 1 ZN ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate 0.000584703 2 EHZ CHOLIC ACID 0.000408571 1 CHD MYRISTIC ACID 0.000228371 1 MYR singleParticle particle 5.1 7.5 10.0 C7H15NO4S MOPS [3-(N-morpholino)propanesulfonic acid] 50.0 KCl Potassium chloride UltrAuFoil GOLD GLOW DISCHARGE 90

Following glow discharge for 90 s at 20 mA, the grid was treated for 48 hours in an anaerobic glovebox in ethanol containing 5 mM 11-mercaptoundecyl hexaethyleneglycol, washed three times in ethanol and dried prior to blotting

ETHANE 100 277 FEI VITROBOT MARK IV

Mix with NADH, then blot for 10 seconds before plunging.

TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 0.9 2.3000000000000003 81000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 20

AutoStigmate and AutoComa on EPU, no AutoCTF

GATAN K3 BIOQUANTUM (6k x 4k) 1 13056 2.14 40.29 1 2721931 RELION 3.1.0 CTFFIND 4.1 PHASE FLIPPING AND AMPLITUDE CORRECTION EMD-14127 C1 2.61 FSC 0.143 CUT-OFF RELION 3.1 29360 PROJECTION MATCHING RELION 3.1.0 MAXIMUM LIKELIHOOD RELION 3.1.0 RELION 3.1.0 emd_55032.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 482.40002 482.40002 482.40002 90.0 90.0 90.0 Z Y X -12.362477 34.319637 -0.00049115904 0.9467671 0.75375 0.75375 0.75375 4.5 AUTHOR ::::EMDATABANK.org::::EMD-55032:::: Locally sharpened consensus map generated using Phenix Autosharpen 7QSK PDB experimental model REAL emd_55032_msk_1.map emd_55032_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 482.40002 482.40002 482.40002 90.0 90.0 90.0 X Y Z -0.027719682 0.08269127 -0.00012556695 0.0021821111 0.75375 0.75375 0.75375 AUTHOR ::::EMDATABANK.org::::EMD-55032:::: Consensus fullmap generated using RELION Refine3D (unsharpened and unfiltered) emd_55032_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 482.40002 482.40002 482.40002 90.0 90.0 90.0 X Y Z -0.07741276 0.15505692 -0.0001218765 0.0065999543 0.75375 0.75375 0.75375 AUTHOR ::::EMDATABANK.org::::EMD-55032:::: Globally sharpened consensus map generated using RELION Postprocess emd_55032_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 482.40002 482.40002 482.40002 90.0 90.0 90.0 X Y Z -0.049701776 0.11274586 -0.0001233746 0.0058108815 0.75375 0.75375 0.75375 AUTHOR ::::EMDATABANK.org::::EMD-55032:::: Half map 1 generated using RELION Refine3D (unsharpened and unfiltered) emd_55032_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 482.40002 482.40002 482.40002 90.0 90.0 90.0 X Y Z -0.050752074 0.10003918 -0.000120378405 0.0059478367 0.75375 0.75375 0.75375 AUTHOR ::::EMDATABANK.org::::EMD-55032:::: Half map 2 generated using RELION Refine3D (unsharpened and unfiltered)