2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-09-26 2025-12-03 2025-12-03 2026-03-18 German Research Foundation (DFG) 448415290 Germany Michael J. Fox Foundation MJFF-010458 United States Aligning Science Across Parkinsons (ASAP) ASAP-000282 United States German Research Foundation (DFG) EXC 2067 Germany Subtomogram average of the mtHsp60 singlering complex from HeLa Ehses K Fernandez-Busnadiego R Mitochondria, human, HeLa, folding, Chaperonin, CHAPERONE Ehses K Lopez-Alonso JP Antico O Lang Y Rudack T Azem A Muqit MMK Ubarretxena-Belandia I Fernandez-Busnadiego R Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress. Sci Adv US 12 eaed3579 eaed3579 2026 41779846 doi:10.1126/sciadv.aed3579 2375-2548 EMDB EMD-55205 associated EM volume HeLa HeLa 0
PINK1-GFP Knockin
Homo sapiens subtomogramAveraging cell 7.0
Addition of 10% Glycerol immediately before plunging into the medium for improved vitrification.
 Quantifoil R2/2 GOLD 200 GOLD HOLEY PLASMA CLEANING ETHANE-PROPANE 310 FEI VITROBOT MARK IV
Backside blotting.
Cells directly grown on EM grids
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 3.0 5.0 53000.0 FEI FALCON IV (4k x 4k) 4.0
For subtomogram averaging, only the first 20 frames were used. The total dose per tilt-series equals 150e/A2.
 1 C7 13.67 FSC 0.143 CUT-OFF Warp Reconstruction by M 18144 242 121000 Dynamo For template matching MATLAB For extraction and parsing
Initial extract carried out with Dynamo template-matching. Then, another round of extraction was performed using Deepfinder.
 Warp 1.0.9 PHASE FLIPPING AND AMPLITUDE CORRECTION RELION 4.0 MAXIMUM LIKELIHOOD RELION 4.0 Final 3D refinement before M Warp Pose optimization in M emd_55205.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 130 130 130 0 0 0 130 130 130 300.612 300.612 300.612 90.0 90.0 90.0 X Y Z -0.0014172997 0.0040100953 -0.0000033517433 0.0003359031 2.3124 2.3124 2.3124 0.000854 AUTHOR Combined FSC-filtered map emd_55205_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 130 130 130 0 0 0 130 130 130 300.612 300.612 300.612 90.0 90.0 90.0 X Y Z -0.0033971402 0.0050168405 -0.000004098125 0.00041827877 2.3124 2.3124 2.3124 AUTHOR Unfiltered Halfset 1 map emd_55205_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 130 130 130 0 0 0 130 130 130 300.612 300.612 300.612 90.0 90.0 90.0 X Y Z -0.0047802003 0.005008124 -0.000002605362 0.00041682538 2.3124 2.3124 2.3124 AUTHOR Unfiltered Halfset 2 map