2026-03-04 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-10-15 2026-02-25 2026-02-25 2026-03-04 Max Planck Society Germany European Molecular Biology Organization (EMBO) ALT 836-2020 European Union European Research Council (ERC) 101054512 European Union Nutrient deprived rat neuronal 80S ribosome - consensus Schwarz A Schuman EM Complex, Translation, Neuron, RIBOSOME Schwarz A Mueller M Will H Dietrich L Giandomenico SL Tushev G Bartnik I Khusainov I Fusco CM Schuman EM Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes. Science US 391 eadr4287 eadr4287 2026 41712727 doi:10.1126/science.adr4287 1095-9203 0038 SCIEAS EMDB EMD-55374 associated EM volume Primary cultured rat hippocampal neurons Primary cultured rat hippocampal neurons 0
nutrient deprived (E4 Buffer)
Rattus norvegicus brain hippocampus subtomogramAveraging cell 7.4
120 mM NaCl, 3 mM KCl, 10 mM D-Glucose, 10 mM HEPES (1 M solution, Gibco 15630-056), pH adjusted to 7.4 with 1 M NaOH, supplemented with 2 mM MgSO4 and 2 mM CaCl2 on the day of the experiment), lacking the amino acids, vitamins and other nutrients present in B27 and GlutaMAX
 Quantifoil R2/2 GOLD 200 CARBON HOLEY GLOW DISCHARGE ETHANE 70 310 LEICA EM GP
10s blotting time, Whatman filter paper #1.
This sample was grown on EM grids, coated with poly-D-lysine, at a density of 140,000 cells/ml in E4 buffer (nutrient deprived)
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 2.5 4.5 33000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 90.0 95.0 GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 0.45 2.1 1 C1 BACK PROJECTION 5.4 FSC 0.143 CUT-OFF RELION 3.1 M 1.0.9 183530 341 183530 low resolution STA of manually picked particles volumes picked interactively Warp 1.0.9
picked in deconvolved tomograms
 Warp 1.0.9 RELION 3.1 PHASE FLIPPING AND AMPLITUDE CORRECTION RELION 3.1 MAXIMUM LIKELIHOOD RELION 3.1 emd_55374.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 168 168 168 0 0 0 168 168 168 450.576 450.576 450.576 90.0 90.0 90.0 X Y Z -0.3514298 0.7934207 0.0057519437 0.041443285 2.682 2.682 2.682 0.11 AUTHOR emd_55374_msk_1.map emd_55374_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 168 168 168 0 0 0 168 168 168 450.576 450.576 450.576 90.0 90.0 90.0 X Y Z -0.27718323 1.0635289 0.02482773 0.111619584 2.682 2.682 2.682 AUTHOR emd_55374_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 168 168 168 0 0 0 168 168 168 450.576 450.576 450.576 90.0 90.0 90.0 X Y Z -0.28874612 1.0373741 0.024758972 0.11151014 2.682 2.682 2.682 AUTHOR