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    <admin>
        <current_status>
            <date>2025-12-24</date>
            <code>REL</code>
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        </current_status>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                        <provider>REPOSITORY</provider>
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                        <provider>REPOSITORY</provider>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </primary_map>
                </change_list>
            </revision>
        </revision_history>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-10-15</deposition>
            <header_release>2025-10-22</header_release>
            <map_release>2025-10-22</map_release>
            <update>2025-12-24</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>R35GM130286</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Swiss National Science Foundation</funding_body>
                <country>Switzerland</country>
            </grant_reference>
        </grant_support>
        <title>Tomogram of a mouse tracheal epithelial cell containing the C2CD3 luminal ring protein</title>
        <authors_list>
            <author ORCID="0000-0003-4978-8516">van den Hoek HG</author>
            <author ORCID="0000-0002-0872-4527">McCafferty C</author>
            <author ORCID="0000-0003-4247-4303">Righetto RD</author>
            <author ORCID="0000-0002-0671-6582">Stearns T</author>
            <author ORCID="0000-0002-0941-4387">Engel BD</author>
        </authors_list>
        <keywords>cilia, flagella, epithelium, transition zone, centriole, TRANSPORT PROTEIN, STRUCTURAL PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Bertiaux E</author>
                    <author order="2">Louvel V</author>
                    <author order="3">McCafferty CL</author>
                    <author order="4">van den Hoek H</author>
                    <author order="5">Batman U</author>
                    <author order="6">Mukherjee S</author>
                    <author order="7">Bournonville L</author>
                    <author order="8">Mercey O</author>
                    <author order="9">Mean I</author>
                    <author order="10">Righetto RD</author>
                    <author order="11">Muller A</author>
                    <author order="12">Van der Stappen P</author>
                    <author order="13">Buss G</author>
                    <author order="14">Daraspe J</author>
                    <author order="15">Genoud C</author>
                    <author order="16">Stearns T</author>
                    <author order="17">Engel BD</author>
                    <author ORCID="0000-0001-5092-2343" order="18">Hamel V</author>
                    <author ORCID="0000-0002-0363-1049" order="19">Guichard P</author>
                    <title>The luminal ring protein C2CD3 acts as a radial in-to-out organizer of the distal centriole and appendages.</title>
                    <journal_abbreviation>Plos Biol.</journal_abbreviation>
                    <country>US</country>
                    <volume>23</volume>
                    <first_page>e3003519</first_page>
                    <last_page>e3003519</last_page>
                    <year>2025</year>
                    <external_references type="PUBMED">41364719</external_references>
                    <external_references type="DOI">doi:10.1371/journal.pbio.3003519</external_references>
                    <external_references type="ISSN">1545-7885</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-55386</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Mouse epithelial tracheal cell</name>
        <supramolecule_list>
            <cell_supramolecule supramolecule_id="1">
                <name>Mouse epithelial tracheal cell</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                    <strain>wild-type CD-1 or GFPcentrin2 transgenic mice</strain>
                    <organ>Trachea</organ>
                    <tissue>Epithelium</tissue>
                </natural_source>
            </cell_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>cell</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>7.0</ph>
                    </buffer>
                    <grid>
                        <model>Quantifoil</model>
                        <material>COPPER</material>
                        <mesh>200</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE-PROPANE</cryogen_name>
                        <instrument>LEICA EM GP</instrument>
                    </vitrification>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage>30</voltage>
                            <current>0.03</current>
                            <duration>2700</duration>
                            <temperature units="K">91</temperature>
                            <initial_thickness>13000</initial_thickness>
                            <final_thickness>130</final_thickness>
                            <details>The value given for _em_focused_ion_beam.instrument is FEI Aquilos. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file.</details>
                        </focused_ion_beam>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">1.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">5.0</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">2.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <algorithm>BACK PROJECTION</algorithm>
                    <software_list>
                        <software>
                            <name>IMOD</name>
                        </software>
                    </software_list>
                    <number_images_used>59</number_images_used>
                </final_reconstruction>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>IMOD</name>
                        </software>
                    </software_list>
                    <type>PHASE FLIPPING ONLY</type>
                </ctf_correction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
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        <file>emd_55386.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>928</col>
            <row>928</row>
            <sec>464</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>-232</sec>
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        <spacing>
            <x>928</x>
            <y>928</y>
            <z>464</z>
        </spacing>
        <cell>
            <a units="Å">13066.24</a>
            <b units="Å">13066.24</b>
            <c units="Å">6533.12</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-2091.416499999999814</minimum>
            <maximum>1486.397200000000112</maximum>
            <average>54.704666000000003</average>
            <std>151.60408000000001</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">14.08</x>
            <y units="Å">14.08</y>
            <z units="Å">14.08</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-55386::::</label>
        <annotation_details>Raw tomogram reconstructed by filtered backprojection with IMOD.</annotation_details>
    </map>
    <interpretation>
        <additional_map_list>
            <additional_map format="CCP4" size_kbytes="399590">
                <file>emd_55386_additional_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS SIGNED BYTE</data_type>
                <dimensions>
                    <col>928</col>
                    <row>928</row>
                    <sec>464</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>928</x>
                    <y>928</y>
                    <z>464</z>
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                <cell>
                    <a units="Å">13066.24</a>
                    <b units="Å">13066.24</b>
                    <c units="Å">6533.12</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
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                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
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                <statistics>
                    <minimum>-128.0</minimum>
                    <maximum>127.0</maximum>
                    <average>-0.9999202</average>
                    <std>10.003857999999999</std>
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                <pixel_spacing>
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                    <y units="Å">14.08</y>
                    <z units="Å">14.08</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-55386::::</label>
                <annotation_details>Tomogram denoised by cryo-CARE.</annotation_details>
            </additional_map>
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                <file>emd_55386_additional_2.map.gz</file>
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                </symmetry>
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                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
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                <spacing>
                    <x>928</x>
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                <cell>
                    <a units="Å">13066.24</a>
                    <b units="Å">13066.24</b>
                    <c units="Å">6533.12</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-128.0</minimum>
                    <maximum>127.0</maximum>
                    <average>-1.0000706</average>
                    <std>10.0039</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">14.08</x>
                    <y units="Å">14.08</y>
                    <z units="Å">14.08</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-55386::::</label>
                <annotation_details>Tomogram denoised by IsoNet on top of cryo-CARE.</annotation_details>
            </additional_map>
        </additional_map_list>
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