2025-12-17 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-12-01 2025-12-17 2025-12-17 2025-12-17 Foundation for Polish Science POIR.04.04.00-00-5B54/17-00 Poland Foundation for Polish Science TEAM TECH CORE FACILITY/2017-4/6 Poland Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with bound plastocyanin Pietras R Sarewicz M Szwalec M Indyka P Rawski M Pintscher S Mielecki B Jaciuk M Koziej L Glatt S Osyczka A Osyczka A b6f complex, photosynthesis, membrane protein, electron transport, proton transport, quinone catalysis, plastocyanin, OXIDOREDUCTASE Sarewicz M Szwalec M Pintscher S Indyka P Rawski M Pietras R Mielecki B Koziej L Jaciuk M Glatt S Osyczka A High-resolution cryo-EM structures of plant cytochrome b6f at work Sci Adv US 9 eadd9688 2023 36638176 doi:10.1126/sciadv.add9688 2375-2548 EMD-14123 other EM volume
Cryo-EM map of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones
 9tgg FULLOVERLAP PDB 7QRM unspecified
Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones
 EMDB EMD-14123 other EM volume
Cryo-EM map of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones
 EMDB EMD-55898 associated EM volume
Cryo-EM structure of Spinacia oleracea cytochrome b6f complex with bound plastocyanin
 cytochrome b6f complex incubated with plastocyanin cytochrome b6f complex incubated with plastocyanin 0 1 2 3 4 5 6 7 8 Spinacia oleracea leaf 0.23 Cytochrome b6 Spinacia oleracea 0.024186504 2 LEVO MSKVYDWFEERLEIQAIADDITSKYVPPHVNIFYCLGGITLTCFLVQVATGFAMTFYYRPTVTDAFASVQYIMTEVNFGW LIRSVHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVTGVVLGVLTASFGVTGYSLPWDQIGYWAVKIVTGVPDAIPV IGSPLVELLRGSASVGQSTLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPL P00165 Cytochrome b6-f complex subunit 4 Spinacia oleracea leaf 0.017456661999999998 2 LEVO MGVTKKPDLNDPVLRAKLAKGMGHNYYGEPAWPNDLLYIFPVVILGTIACNVGLAVLEPSMIGEPADPFATPLEILPEWY FFPVFQILRTVPNKLLGVLLMASVPAGLLTVPFLENVNKFQNPFRRPVATTVFLVGTVVALWLGIGATLPIDKSLTLGLF P00166 Cytochrome f Spinacia oleracea leaf 0.03135702 2 LEVO YPIFAQQGYENPREATGRIVCANCHLANKPVDIEVPQAVLPDTVFEAVVRIPYDMQLKQVLANGKKGGLNVGAVLILPEG FELAPPDRISPEMKEKMGNLSFQSYRPNKQNILVIGPVPGQKYSEITFPILAPDPATKKDVHFLKYPIYVGGNRGRGQIY PDGSKSNNTVYNSTATGIVKKIVRKEKGGYEINIADASDGREVVDIIPRGPELLVSEGESIKLDQPLTSNPNVGGFGQGD AEVVLQDPLRIQGLLFFFASVILAQIFLVLKKKQFEKVQLSEMNF P16013 Cytochrome b6-f complex iron-sulfur subunit, chloroplastic Spinacia oleracea 0.018956479999999998 2 LEVO ATSIPADNVPDMQKRETLNLLLLGALSLPTGYMLLPYASFFVPPGGGAGTGGTIAKDALGNDVIAAEWLKTHAPGDRTLT QGLKGDPTYLVVESDKTLATFGINAVCTHLGCVVPFNAAENKFICPCHGSQYNNQGRVVRGPAPLSLALAHCDVDDGKVV FVPWTETDFRTGEAPWWSA P08980 7.1.1.6 Cytochrome b6-f complex subunit 6 Spinacia oleracea leaf 0.0034521999999999995 2 LEVO MFTLTSYFGFLLAALTITSALFIGLNKIRLI Q9M3L0 Cytochrome b6-f complex subunit 7 Spinacia oleracea 0.003774431 2 LEVO NAAAEIFRIAAVMNGLTLVGVAIGFVLLRIEATVEE P80883 Cytochrome b6-f complex subunit 5 Spinacia oleracea leaf 0.004171985 2 LEVO MIEVFLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDL P69461 Cytochrome b6-f complex subunit 8 Spinacia oleracea leaf 0.003170809 2 LEVO MDIVSLAWAALMVVFTFSLSLVVWGRSGL P61045 Plastocyanin, chloroplastic Spinacia oleracea leaf 0.010420557 1 LEVO VEVLLGGGDGSLAFLPGDFSVASGEEIVFKNNAGFPHNVVFDEDEIPSGVDAAKISMSEEDLLNAPGETYKVTLTEKGTY KFYCSPHQGAGMVGKVTVN P00289 PROTOPORPHYRIN IX CONTAINING FE 0.000616487 4 HEM HEME C 0.0006185030000000001 4 HEC UNDECYL-MALTOSIDE 0.000496589 6 UMQ 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE 0.000749201 7 PL9 CHLOROPHYLL A 0.000893489 2 CLA FE2/S2 (INORGANIC) CLUSTER 0.00017581999999999998 2 FES 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL 0.0007951159999999999 2 SQD BETA-CAROTENE 0.000536873 2 BCR COPPER (II) ION 6.354599999999999e-05 1 CU singleParticle particle 9.5 7.5 10.0 C4H11NO3 TRIS-HCL 15.0 NaCl sodium chloride 0.7 C23H44O11 n-undecyl beta-maltoside Quantifoil R2/1 COPPER 200 CARBON HOLEY GLOW DISCHARGE AIR ETHANE 100 277 FEI VITROBOT MARK IV
final concentration of cytochrome b6f dimers and plastocyanin was 38.7 uM and 100 uM, respectively
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.9 2.1 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 5760 4092 1 7784 40.0 1 177412
template picker
 cryoSPARC 3.3.1 PHASE FLIPPING AND AMPLITUDE CORRECTION
Ab-initio
 1 FOURIER SPACE 3.18 FSC 0.143 CUT-OFF RELION 3.1.2 87892 MAXIMUM LIKELIHOOD cryoSPARC 3.3.1 NOT APPLICABLE 3 32532.0 RELION 3.1.2
3D classification
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