2026-02-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2026-01-11 2026-02-18 2026-02-18 2026-02-18 Aligning Science Across Parkinsons (ASAP) ASAP-000282/ASAP-024268 United States In situ cryo-ET tomogram of lysosome damaged by LLOMe (0.5mM, 60min) in HeLa TMEM192-3xHA cell. Li D Wilfling F Lysosome, HeLa cell, Endolysomal system, organelle, ENDOCYTOSIS Li D Zhang W Medina M Stuke JFM Schwarz A Brill J Brenner J Kraus F Ohlerich S Lizarrondo J Pflaum J Grass JH Soltow LM Hammerschmid D Weber N Welsch S Langer JD Windbergs M Harper JW Schuman E Hummer G Grotjahn DA Wilfling F Cathepsin-dependent amyloid formation drives mechanical rupture of lysosomal membranes. Biorxiv US 2026 41648416 doi:10.64898/2026.01.17.700056 2692-8205 EMDB EMD-56296 associated EM volume HeLa TMEM192-3xHA cell HeLa TMEM192-3xHA cell 0
treated with LLOMe (Cayman Chemicals, 16008) at 0.5mM for 60min.
Homo sapiens HeLa tomography cell 7.4 Quantifoil R1/4 GOLD 200 SILICON DIOXIDE HOLEY ETHANE 70 310 LEICA EM GP
treated with LLOMe (Cayman Chemicals, 16008) at 0.5mM for 60min.
Dynabeads Thermo Fisher Scientific, acid65011 1 OTHER OTHER 30 0.5 120 80 1000 130
using AutoTEM. The value given for _em_focused_ion_beam.instrument is Aquilos 2 FIB/SEM. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 2.5 5.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) 2.5 1 IMOD 4.12.62 AreTomo 1.0.0 AreTomo2, Denoising done in Isonet2 61 Gctf PHASE FLIPPING ONLY emd_56296.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1022 1440 550 0 0 0 1022 1440 550 8895.488 12533.761 4787.2 90.0 90.0 90.0 X Y Z -11.514920999999999 2.5776496 0.0005319018 0.10774501 8.704 8.704 8.704 AUTHOR In situ cryo-ET tomogram of damaged lysosome by LLOMe (0.5mM, 60min) in HeLa TMEM192-3xHA cell