2026-02-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2026-01-12 2026-02-18 2026-02-18 2026-02-18 Aligning Science Across Parkinsons (ASAP) ASAP-000282/ASAP-024268 United States In situ cryo-ET tomogram of lysosomal structure in LLOMe-treated (0.5mM, 1h) rat hippocampal neuron. Li D Schwarz A Wilfling F Lysosome, Endolysosomal system, neuron, rat, ENDOCYTOSIS Li D Zhang W Medina M Stuke JFM Schwarz A Brill J Brenner J Kraus F Ohlerich S Lizarrondo J Pflaum J Grass JH Soltow LM Hammerschmid D Weber N Welsch S Langer JD Windbergs M Harper JW Schuman E Hummer G Grotjahn DA Wilfling F Cathepsin-dependent amyloid formation drives mechanical rupture of lysosomal membranes. Biorxiv US 2026 41648416 doi:10.64898/2026.01.17.700056 2692-8205 EMDB EMD-56330 associated EM volume Rat hippocampal neuron Rat hippocampal neuron 0
rat hippocampal neurons were prepared from P0 or P1 rat pups (Sprague Dawley, IGS, Crl:CD(SD), Charles River Laboratories, RRID:RGD 734476) treated with 0.5mM LLOMe for 1h.
Rattus Brain Hippocampus tomography cell 7.4 Quantifoil R1/4 GOLD 200 SILICON DIOXIDE HOLEY ETHANE 70 310 LEICA EM GP OTHER OTHER 30 0.5 120 80 1000 130
The value given for _em_focused_ion_beam.instrument is TFS Aquilos 2 FIB/SM. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 4.0 7.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris X 10 TFS FALCON 4i (4k x 4k) 2.5 1 IMOD 4.12 60 PHASE FLIPPING ONLY emd_56330.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1024 1024 500 0 0 0 1024 1024 500 12441.6 12441.6 6075.0 90.0 90.0 90.0 X Y Z -29.660606000000001 9.042104 -0.016753549 0.21892938 12.15 12.15 12.15 AUTHOR In situ cryo-ET tomogram of lysosomal structure in LLOMe-treated (0.5mM, 1h) rat hippocampal neuron.