2026-02-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update PDBe PDBe 2026-01-19 2026-02-11 2026-02-11 2026-02-18 Not funded Structure of the MAP2K MEK1 in an inactive conformation in complex with its substrate MAPK ERK2 von Velsen J Juyoux P Bowler MW protein kinases, phosphoryl transfer, MAPK, MAP2K, cancer signaling, SIGNALING PROTEIN von Velsen J Juyoux P Piasentin N Fisher H Lapouge K Vadas O Gervasio FL Bowler MW Molecular basis of mitogen-activated protein kinase ERK2 activation by its upstream kinase MEK1. Biorxiv US 2026 41648251 doi:10.64898/2026.01.19.700303 2692-8205 EMD-56419 other EM volume
other reconstruction originate from the same data collection
 EMD-56420 other EM volume
other reconstruction originate from the same data collection
 9tyg FULLOVERLAP EMDB EMD-56419 other EM volume
other reconstruction originate from the same data collection
 EMDB EMD-56420 other EM volume
other reconstruction originate from the same data collection
 EMDB EMD-56418 associated EM volume
Structure of the MAP2K MEK1 in an inactive conformation in complex with its substrate MAPK ERK2
 Complex between the MAP2K MEK1 and its substrate MAPK ERK2 Complex between the MAP2K MEK1 and its substrate MAPK ERK2 0 1 2 Homo sapiens 41.7 MEK1DDGRA 1 1
human MEK1 with KIM motif exchanged to GRA24 KIM (15 residues).
Homo sapiens ERK2 1 2
human ERK2 with point mutation on T185V.
Homo sapiens Dual specificity mitogen-activated protein kinase kinase 1,Uncharacterized protein,Dual specificity mitogen-activated protein kinase kinase 1 Homo sapiens 0.044981561999999996
protein chimera of human MEK1, exchanging native KIM (r3-11) by GRA24 KIM (15 residues). Addition of activating mutations on serines in activation-loop: Ser218Asp, Ser222Asp.,protein chimera of human MEK1, exchanging native KIM (r3-11) by GRA24 KIM (15 residues). Addition of activating mutations on serines in activation-loop: Ser218Asp, Ser222Asp.,protein chimera of human MEK1, exchanging native KIM (r3-11) by GRA24 KIM (15 residues). Addition of activating mutations on serines in activation-loop: Ser218Asp, Ser222Asp.,protein chimera of human MEK1, exchanging native KIM (r3-11) by GRA24 KIM (15 residues). Addition of activating mutations on serines in activation-loop: Ser218Asp, Ser222Asp.,protein chimera of human MEK1, exchanging native KIM (r3-11) by GRA24 KIM (15 residues). Addition of activating mutations on serines in activation-loop: Ser218Asp, Ser222Asp.,protein chimera of human MEK1, exchanging native KIM (r3-11) by GRA24 KIM (15 residues). Addition of activating mutations on serines in activation-loop: Ser218Asp, Ser222Asp.
1 Trichoplusia ni LEVO MPLLERRGVSELPPLYINPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISEL GAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQV LKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDDMANDFVGTRSYMSPER LQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELL DYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGVL EVLFQ Q02750 A0A125YG37 Q02750 2.7.12.2 Mitogen-activated protein kinase 1 Homo sapiens 0.041724976999999996
ERK2 with point mutation on residue 185 to a valine
1 Escherichia coli LEVO QGPMAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLR FRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLL NTTCDLKICDFGLARVADPDHDHTGFLVEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQL NHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYY DPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS P28482 2.7.11.24 ADENOSINE-5'-DIPHOSPHATE 0.000427201 2 ADP MAGNESIUM ION 2.4305e-05 1 MG singleParticle particle 0.516 7.5 50.0 HEPES pH 7.5 200.0 NaCl sodium chloride 10.0 MgCl2 magnesium chloride 5.0 Glycerol 0.5 TCEP HexAuFoil GOLD GOLD HOLEY PLASMA CLEANING OTHER ETHANE 100 279.15 FEI VITROBOT MARK IV
250 uM ADP.AlF4- was added to the sample
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 2.2 270000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris X 10 TFS FALCON 4i (4k x 4k) 1 88517 1.7 50.0 1 cryoSPARC v4.7.0 Patch CTF PHASE FLIPPING AND AMPLITUDE CORRECTION AlphaFold 2.9 FSC 0.143 CUT-OFF cryoSPARC v4.7.0 142124 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_56418.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 220 220 220 0 0 0 220 220 220 202.40001 202.40001 202.40001 90.0 90.0 90.0 X Y Z -0.20678288 0.5856031 0.0006000431 0.0128877675 0.92 0.92 0.92 0.0669 AUTHOR refined map of MEK1DDGRA-ERK2T185V AlphaFold in silico model RIGID BODY FIT REAL emd_56418_msk_1.map emd_56418_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 220 220 220 0 0 0 220 220 220 202.40001 202.40001 202.40001 90.0 90.0 90.0 X Y Z -0.2759306 0.7205638 0.00077336375 0.014220664 0.92 0.92 0.92 AUTHOR B-factor sharpened map of MEK1DDGRA-T185V emd_56418_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 220 220 220 0 0 0 220 220 220 202.40001 202.40001 202.40001 90.0 90.0 90.0 X Y Z -0.3910495 0.7068123 0.000504464 0.052621096 0.92 0.92 0.92 AUTHOR half map B emd_56418_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 220 220 220 0 0 0 220 220 220 202.40001 202.40001 202.40001 90.0 90.0 90.0 X Y Z -0.41755557 0.757493 0.0005045392 0.053354505 0.92 0.92 0.92 AUTHOR half map A