REL RCSB RCSB RCSB 2014-01-22 2014-02-19 2014-03-05 2015-10-07 Cryo-EM structure of MAVS CARD filament Xu H He X Zheng H Huang LJ Hou F Yu Z de la Cruz MJ Borkowski B Zhang X Chen ZJ Jiang Q-X Innate immunity, helical filament Xu H He X Zheng H Huang LJ Hou F Yu Z de la Cruz MJ Borkowski B Zhang X Chen ZJ Jiang QX Structural basis for the prion-like MAVS filaments in antiviral innate immunity. eLife 3 e01489 e01489 2014 24569476 doi:10.7554/eLife.01489 3j6c FULLOVERLAP http://dx.doi.org/10.6019/EMPIAR-10014 MAVS CARD filament MAVS CARD filament polymer 1 0.546 Mitochondrial antiviral-signaling protein (MAVS) Homo sapiens Human 0.013 polymer true Homo sapiens HEK293T pcDNA3 Q7Z434 helical filament 7.5
20mM Tris-HCl, 50mM NaCl, 1mM DTT
Quantifoil grids coated with thin carbon on top
 ETHANE 100 FEI VITROBOT MARK III JEOL 2200FS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 0.8 2.0 60000.0 61950.0 GATAN LIQUID NITROGEN Objective lens astigmatism was corrected at 100,000x magnification JEOL omega filter 0.0 35.0 2011-10-20 KODAK SO-163 FILM ZEISS SCAI 7 20
IHRSR
16.8 53.6 C3 OTHER 9.6 OTHER Spider, MRC, EMAN, IMAGIC4D
Final data were calculated from three separate datasets from three sessions of data collection. The handedness of the map was determined by cryo-electron tomography.
Each filament segment
 emd_5890.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 50 50 100 0 0 0 50 50 100 116.649994 116.649994 233.29999 90.0 90.0 90.0 X Y Z -4.69236183 6.60222197 0.36144659 0.89477062 2.333 2.333 2.333 0.52 AUTHOR Cryo-EM map of MAVS CARD filament
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 2VGQ A RIGID BODY FIT Chimera, SITUS
The docking of one X-ray model into a segmented map corresponding to one subunit was first done manually in Chimera, and then optimized using SITUS.
REAL
emd_5890.tif