REL RCSB RCSB RCSB 2014-05-21 2014-07-30 2014-08-06 2015-10-07 Structures of yeast 80S ribosome-tRNA complexes in the rotated and non-rotated conformations (Class II - 1 tRNA in rotated conformation) Svidritskiy E Brilot AF Koh CS Grigorieff N Korostelev AA 80S ribosome, Kozak sequence, translation Svidritskiy E Brilot AF Koh CS Grigorieff N Korostelev AA Structures of Yeast 80S Ribosome-tRNA Complexes in the Rotated and Nonrotated Conformations. STRUCTURE 22 1210 1218 2014 25043550 doi:10.1016/j.str.2014.06.003 EMD-5977 FULLOVERLAP 3j77 FULLOVERLAP http://dx.doi.org/10.6019/EMPIAR-10016 80S ribosome bound to mRNA containing Kozak sequence and to one tRNA 80S ribosome bound to mRNA containing Kozak sequence and to one tRNA
Sample was monodisperse.
3 3.5 80S ribosome false Saccharomyces cerevisiae Yeast 3.5 ribosome-eukaryote: ALL mRNA Saccharomyces cerevisiae 0.005 AAAAAUGUAAAAAA OTHER SINGLE STRANDED true transfer RNA Escherichia coli 0.025
tRNA fmet
TRANSFER OTHER false singleParticle particle 1.2 7.5
20 mM Tris-HCl, 50 mM NH4Cl, 20 mM MgCl2, 0.3 U/uL RNasin
 ETHANE 95 FEI VITROBOT MARK II FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.01 1.159 4.844 133333.0 133333.0 FEI TITAN KRIOS AUTOGRID HOLDER 2013-01-02 FEI FALCON I (4k x 4k) 14 4754 30 16.
CTFFIND3, FREALIGN per micrograph
 OTHER 6.2 OTHER EMAN2, IMAGIC, FREALIGN, RSAMPLE, CTFFIND3 25136 emd_5976.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 377.99997 377.99997 377.99997 90.0 90.0 90.0 X Y Z -0.18186775 0.47021329 0.00601077 0.04798295 1.05 1.05 1.05 0.12 AUTHOR Reconstruction of a yeast 80S ribosome in the rotated state with 1 tRNA bound. (Class II)
::::EMDATABANK.org::::EMD-5976::::
 3U5B RIGID BODY FIT Chimera, CNS
3U5B, 3U5C, 3U5D, and 3U5E were combined prior to fitting. tRNA and mRNA were modeled using individual tRNA and mRNA from the crystal structure (4GD1) of the hybrid-state 70S ribosome containing P/E tRNA. The structure of rpL1 was obtained by homology modeling from PDB ID 3J3B.
cross-correlation REAL 3U5C RIGID BODY FIT Chimera, CNS
3U5B, 3U5C, 3U5D, and 3U5E were combined prior to fitting. tRNA and mRNA were modeled using individual tRNA and mRNA from the crystal structure (4GD1) of the hybrid-state 70S ribosome containing P/E tRNA. The structure of rpL1 was obtained by homology modeling from PDB ID 3J3B.
cross-correlation REAL 3U5D RIGID BODY FIT Chimera, CNS
3U5B, 3U5C, 3U5D, and 3U5E were combined prior to fitting. tRNA and mRNA were modeled using individual tRNA and mRNA from the crystal structure (4GD1) of the hybrid-state 70S ribosome containing P/E tRNA. The structure of rpL1 was obtained by homology modeling from PDB ID 3J3B.
cross-correlation REAL 3U5E RIGID BODY FIT Chimera, CNS
3U5B, 3U5C, 3U5D, and 3U5E were combined prior to fitting. tRNA and mRNA were modeled using individual tRNA and mRNA from the crystal structure (4GD1) of the hybrid-state 70S ribosome containing P/E tRNA. The structure of rpL1 was obtained by homology modeling from PDB ID 3J3B.
cross-correlation REAL 4GD1 V X RIGID BODY FIT Chimera, CNS
3U5B, 3U5C, 3U5D, and 3U5E were combined prior to fitting. tRNA and mRNA were modeled using individual tRNA and mRNA from the crystal structure (4GD1) of the hybrid-state 70S ribosome containing P/E tRNA. The structure of rpL1 was obtained by homology modeling from PDB ID 3J3B.
cross-correlation REAL 3J3B RIGID BODY FIT Chimera, CNS
3U5B, 3U5C, 3U5D, and 3U5E were combined prior to fitting. tRNA and mRNA were modeled using individual tRNA and mRNA from the crystal structure (4GD1) of the hybrid-state 70S ribosome containing P/E tRNA. The structure of rpL1 was obtained by homology modeling from PDB ID 3J3B.
cross-correlation REAL