2025-11-19 REL PDBj PDBj PDBj 2024-05-28 2025-11-19 2025-11-19 2025-11-19 Other government ZJU100 Young Professor Cryo-EM map of respirasome open state 2 in presence of metformin (SC-MetO2), complex I distal membrane arm focused Teng F He ZX Hu YQ Xu CY Guo RY Zhou L Metformin, electron transport chain, respirasome, mammalia, MEMBRANE PROTEIN He Z Teng F Yang Y Guo R Wu M Han F Tian H Wang J Hu Y Jiang Y Zhang L Xu C Yang F Zhou J Zhang S Letts JA Zhou R Zhou L Hydrophilic metformin and hydrophobic biguanides inhibit mitochondrial complex I by distinct mechanisms. Nat.Struct.Mol.Biol. US 2025 41214295 doi:10.1038/s41594-025-01710-6 1545-9985 EMD-60319 consensus EM volume PDB 8ZOU unspecified EMDB EMD-60319 consensus EM volume EMDB EMD-60296 associated EM volume Respirasome open state 2 in presence of metformin Respirasome open state 2 in presence of metformin 0 Sus scrofa singleParticle particle 0.25 7.4 20.0 Tris Trimethylsilyl 50.0 NaCl Sodium chloride 0.002 PMSF Phenylmethylsulfonyl 0.1 GDN Glyco-diosgenin
SEC buffer (20 mM Tris pH 7.4, 50 mM NaCl, 0.002% PMSF, 0.1% GDN (w/v))
 Quantifoil R1.2/1.3 COPPER 300 CARBON CONTINUOUS 2.0 ETHANE 100 277.15 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.6 1.8 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON IV (4k x 4k) 4096 4096 1 7.8 51.9 1 CTFFIND 4.1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.87 FSC 0.143 CUT-OFF cryoSPARC v.4.4.1 67621 COMMON LINE cryoSPARC v.3.3.2 COMMON LINE cryoSPARC v.3.3.2 cryoSPARC v.4.4.1 emd_60296.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 X Y Z -3.5151813 6.612614 -0.0030675884 0.19686423 1.2 1.2 1.2 1.1 AUTHOR RIGID BODY FIT REAL emd_60296_msk_1.map emd_60296_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 X Y Z -1.7471842 2.8651438 0.0019866896 0.19071677 1.2 1.2 1.2 AUTHOR emd_60296_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 X Y Z -1.4325489 2.6968906 0.0020357033 0.19037159 1.2 1.2 1.2 AUTHOR