Respirasome closed state 1 bound by metformin (SC-MetC1)

2026-02-04 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update PDBj PDBj 2024-06-05 2025-11-19 2025-11-19 2026-02-04 Other government ZJU100 Young Professor Respirasome closed state 1 bound by metformin (SC-MetC1) Teng F He ZX Hu YQ Xu CY Guo RY Zhou L Metformin, electron transport chain, Respirasome, mammalia, MEMBRANE PROTEIN He Z Teng F Yang Y Guo R Wu M Han F Tian H Wang J Hu Y Jiang Y Zhang L Xu C Yang F Zhou J Zhang S Letts JA Zhou R Zhou L Hydrophilic metformin and hydrophobic biguanides inhibit mitochondrial complex I by distinct mechanisms. Nat.Struct.Mol.Biol. US 33 100 111 2026 41214295 doi:10.1038/s41594-025-01710-6 1545-9985 EMD-60306 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1)

EMD-60308 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex I peripheral arm focused

EMD-60309 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex I proximal membrane arm focused

EMD-60310 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex I distal membrane arm focused

EMD-60311 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex III2 focused

EMD-60313 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex IV focused

8zsq FULLOVERLAP EMDB EMD-60306 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1)

EMDB EMD-60308 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex I peripheral arm focused

EMDB EMD-60309 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex I proximal membrane arm focused

EMDB EMD-60310 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex I distal membrane arm focused

EMDB EMD-60311 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex III2 focused

EMDB EMD-60313 other EM volume

Cryo-EM map of respirasome closed state 1 bound by metformin (SC-MetC1), complex IV focused

EMDB EMD-60424 associated EM volume

Respirasome closed state 1 inhibited by metformin

Respirasome closed state 1 inhibited by metformin Respirasome closed state 1 inhibited by metformin 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 Sus scrofa NADH-ubiquinone oxidoreductase chain 4L Sus scrofa 0.010827253 1 LEVO MPLVYMNIIMAFAIALAGLLMYRSHLMSSLLCLEGMMLSLFIMSTLIILNTHFTLANMMPIILLVFAACEAALGLSLLVM VSNTYGTDYVQNLNLLQC P56632 7.1.1.2 Cytochrome c oxidase subunit 5A, mitochondrial Sus scrofa 0.011717334999999999 1 LEVO TDEEFDARWVTYFNKPDIDAWELRKGMNTLVGYDLVPEPKIIDAALRACRRLNDFASAVRILEVVKDKAGPHKEIYPYVI QELRPTLNELGISTPEELGLDK F1SJ34 Cytochrome c oxidase subunit 5B, mitochondrial Sus scrofa 0.010382738999999998 1 LEVO SGGGVPTDEEQATGLEREVMMAARKGLDPYNILAPKAASGTKEDPNLVPSITNKRIVGCICEEDNSTVIWFWVHKGETQR CPSCGTHYKLVSHQL Q5S3G4 Cytochrome c oxidase subunit Sus scrofa 0.008724873999999999 1 LEVO TGARTWRFLTFGLALPSVALCTLNSWLHSGHHHRPEFIPYHHLRIRTKPYSWGDGNHTLFHNPRVNPLPTGYEQP A0A287AEB7 Cytochrome c oxidase subunit Sus scrofa 0.009723962999999999 1 LEVO IQTKIKNYQTAPFDSRFPNQNQTRNCWQNYLDFHRCEKAMTAKGGDVSVCEWYRRVYKSLCPISWVSAWDDRRAEGTFPG KI A0A4X1TG97 Cytochrome c oxidase subunit 6C Sus scrofa 0.008139589 1 LEVO LTKPQMRGLLAKRLRFHIVGAFIVSLGVATFYKFAVAEPRKKAYADFYRNYDSMKDFEEMRKAGIFQSAK A0A4X1TXH1 Cytochrome c oxidase subunit 7A1, mitochondrial Sus scrofa 0.006376323999999999 1 LEVO LENRVAEKQKIFQADNDLPVHLKGGATDNILYRVTMTLCLGGTVYSLYCLGWASFPH Q8SPJ9 Cytochrome c oxidase subunit 7B, mitochondrial Sus scrofa 0.0055432070000000005 1 LEVO APDFHDKYGNAILASGATFCVAVWAYTATQIGIEWNLSPVGRVTPKEWRE A0A287BJ57 Cytochrome c oxidase subunit 7C, mitochondrial Sus scrofa 0.005465395999999999 1 LEVO SHYEEGPGKNLPFSVENKWRLLAMMTLYFGSGFAAPFFIVRHQLLKK Q1W0Y2 Cytochrome c oxidase subunit 8 Sus scrofa 0.004776493000000001 1 LEVO IYAKPARTPTSPTEQAIGLSVTFLSFLIPAGWVLSHLDHYKRS A0A4X1VY94 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Sus scrofa 0.008034373 1 LEVO MWFEILPGIAVMAACLFIPGMATAHIHKFTNGGKEKRVAHFSYQWNLMERDRCISGVNRYHVTKGLENID K7GR43 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Sus scrofa 0.009770203 1 LEVO LGLREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQEKNVSLNNFSADQVTRTLEN VLSGK F1RGE3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 Sus scrofa 0.009094371999999998 1 LEVO AGRIASFLKNAWAKEPVLVASFAIGGLAIILPSLSPYTNYAIRINRATPYNYPVPLRDDGNMPDVPSHPQDPQGPSLEWL KNL A0A4X1VPG4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Sus scrofa 0.012949105999999998 1 LEVO LKKTTGLVGLAVCETPHERLKILYTKILDVLGQIPKNAAYRKYTEQITNEKLGMVKAEPDVKKLEEQLQGGQIEEVILQA ENELSLARKMLRWKPWEPLVEEPPANQWKWPI A0A8D1IJ52 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Sus scrofa 0.013755924000000001 1 LEVO TSVKPIFSRDMNEAKRRVRELYRAWYREVPNTVHLFQLDISVKQGRDKVREMFMKNAHVTDPRVVDLLVIKGKMELEETI NVWKQRTHIMRFFHETEAPRPTDFLSKFYVGHDP F1SJP6 Complex I-B14.5a Sus scrofa 0.012517394 1 LEVO ASATRVIQLLRNWASGRDLQAKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRREAMPPSIVMSSQKVLASGKP AESSAVAETEKKAVTPAPPIKRWELSKDQPYL A0A4X1VLA2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Sus scrofa 0.019932898 1 LEVO PGIVELPTLEDLKVQEVKVSSSVLKAAAHHYGAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNQCALDFFRQIKRHCAEP FTEYWTCIDYSGLQLFRHCRKQQAKFDECVLDKLGWVRPDLGELSKVTKVKTDRPLPENPYHSRARPEPNPEAEGDLKPA KHGSRLFFWTM F1SLR1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Sus scrofa 0.03884089499999999 1 LEVO LHHALIPHGKGGRSSVSGIVATVFGATGFLGRYVVNHLGRMGSQVIVPYRCEPYDTMHLRPMGDLGQIIFMEWNGKDKDS IRKVVEHSNVVINLVGREWETKNFDFEDVFVKIPHAIAQVSKEAGVEKLIHISHLNADIKSPSRYLRSKAVGEKEVRAAF PEATIIKPSDIFGREDRFLNYFASMRWFGGVPLISLGKETVKQPVYIVDVSKGIINAIKDPDAKGKTFAFVGPNRYLLFD LVQYIFAVAYRPFLPYPLPHFAYRWVGRLFEVSPFEPWTTRDKVERVHMSDMTLPHLPGLEDLGIQATPLELKAIEVLRR HRTYRWLTSEMEDVKPAKTVN F1SL07 Acyl carrier protein Sus scrofa 0.010046488 2 LEVO DAPPLTLEAIKDRVLYVLKLYDKIDPEKLSVNSHFMKDLGLDSLDQVEIIMAMEDEFGFEIPDIDAEKLMCPQEIVDYIA DKKDVYE A0A287AAR5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Sus scrofa 0.036949875 1 LEVO RLQYGPLAFVLGERTTRKLTETSKVITVDGNICSGKGRLAREIAEKLGLRHFPEAGIHYADSTTGDGKPLDVQLSGNCSL EKFYDDPKSNDGNSYRLQSWLYASRLLQYADALEHLLSTGQGVVLERSIYSDFVFLEAMYRQGFIRKQCVEHYNEVKKVT ACEYLPPHVVVYVDVPVPEIQSRIQKKGNPHEMKITAAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAEDAEKVVEDIE YLKCDKGPWPDQDDRTFHRLRMLVQNKLEVLNYTTIPVYLPEITIGAHQSDRVFQKFTELPGRKYSPGYNEDVGDKWIWL K F1SIS9 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 Sus scrofa 0.014555697999999999 1 LEVO AKTLLHKYSDIPEGTECHRKAYASTSIGGATGLIVSAYSIALKPPASFLEGVARTGRYTFTSAAIGAIFGLTSCISAQVR EKPDDPLNYFIGGCAGGLTLGARTRSYGIGAAACAYMGLTAALVKMGQLEGWQVFAEPKV I3LGM4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Sus scrofa 0.017031243999999997 1 LEVO ELVQVLRRGLQQVSGHGGLRGYLRVLFRANDVRVGTLVGEDKYGNKYYEDNKQFFGRHRWVIYTTEMNGRDTFWDVDGSM VPPEWHRWLHCMTDDPPTTKPPTARKYIWTNHKFNVSGTPQQYVPYSTTRKKIQEWVPPSTPYK F1SQP4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Sus scrofa 0.01670934 1 LEVO ASKVKQDMPPPGGYGPIDYKRNLPRRGLSGYSMFAVGIGTLLFGYWSMMKWNRERRRLQIEDFEARIALMPLFQAEKDRR VLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVTPMMGELYGLRTNEEILSATYGFIWYT A0A8D1NRT6 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 Sus scrofa 0.006781886 1 LEVO NVLQIVRDHWVHILVPVGFVFGCYLDRRSDEKLTAFRNKSLLFKRELRPNEEVTWK A0A8D2CGH8 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial Sus scrofa 0.008201093 1 LEVO VHIEPRYRQFPQLTRSQLIQAEFFSATMWFWILWRFWHDSDAVLGHFPYPDPSQWTDEELGILPDDE A0A5G2QW32 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 Sus scrofa 0.009042211999999999 1 LEVO SKMELPDYKQWKIEGTPLETVQEKLAARGLRDPWGRNEAWRYSGGFANNVSFVGALLKGFKWGFAAFVVAVGAEYYLESQ A0A4X1VD84 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Sus scrofa 0.014986153 1 LEVO SFPKYKPSRLATLPATLDPAEYDISPETRKAQAERLAIRSRLKREYLLQYNDPNRLGVIEDPALIRWTYARSANIYPNFR PTPKTSLLGALFGIGPLFFWYYVFKTDRDKKEKLIQEGKLDQTFNISY A0A4X1T6M0 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial Sus scrofa 0.016281898 1 LEVO KRLFIIKPSGFYDRRFLKLMRFYILLTGIPVAIGITLVNVFIGEAELADIPEGYVPEHWEYFKHPISRWIARTFYDGPEK NYEKTMAILQIEAEKAELRLKELEVRRLMRARGDGPWYQYPTIDKALIDHSPKTTPDN F1SGC6 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Sus scrofa 0.015333832 1 LEVO SGYTPDEKLRLQQLRELRRRWLKDQELSPREPVLPPRRVWPMEQFWNKFLQDGAPWKNVIYKTYRHSIFAVTHVLIPVWI IHYYLKYHVTAKPYTVVERKPRIFPGDTILETGEVIPLMKEFPDQH A0A480KM09 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Sus scrofa 0.015307553 1 LEVO MGAHLARRYLGDASKEPDPLRMPTFPPDYGFPERKEREMVATQQEMNDAQLMLQQRDYCAHYLIQLLKCKRDSFPNFLAC KHEQHDWDYCEHLDYVKRMKEFERERRLLQRKKRREQREAEMARG F1SCH1 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Sus scrofa 0.018608805 1 LEVO HVTKDMFPGPYPKTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPDRSQQERDPWYDWDHPDLRLNWGEPIHWDLD MYIRNRVDTSPTPVSWNTMCKHLFGFVAFMLFMFWVGEIYPSYQPVGPKQYPYNDLYLERGGDPTKEPEPVVHYEI F1RWL7 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Sus scrofa 0.021733711 1 LEVO AFSAPAAYLTHQQKVLRLYKRALRHLESWCVHRDKYRYFACLMRARFDEHKNEKDMVKATQLLRQAEEEFWYGQHPQPYI FPESPGGTSYERYECYKVPEWCLDDWHPSEKAMYPDYFAKREQWKRLRRESWEREVKQLQEETPPGGPRTEALPPARKEG DLPPLWWHIVTRPRERPM K7GSE5 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 Sus scrofa 0.020742493999999997 1 LEVO PDSWDKDVYPEPPRRTPAPAPQTSLPNPVTYLTKIFDLLVDRPVTLAREFIEQQHAKNRYYYYHREFRRVPDITECEEKD ILCMFEAEMQWRRDYKVDQEIVNIIQERLKACQQREGESYRQNCAKELEQFTQVSKAFQDRYSDLGAHYSARKCLAKQKQ RMLAERKAAKEAAA A0A8D1FAG1 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial Sus scrofa 0.011754205 1 LEVO IRLQEDPDPEDENLYEKNPDSHGYDKDPIVDLWNMRVVFFFGFSIVLVLGSTFVAYLPDYRMQEWARREAERLVKYREAN GLPLMESNCFDPNKIQLPE A0A8D1EYI7 Cytochrome c oxidase subunit 1 Sus scrofa 0.056992711 1 LEVO MFVNRWLYSTNHKDIGTLYLLFGAWAGMVGTALSLLIRAELGQPGTLLGDDQIYNVIVTAHAFVMIFFMVMPIMIGGFGN WLVPLMIGAPDMAFPRMNNMSFWLLPPSFLLLLASSMVEAGAGTGWTVYPPLAGNLAHAGASVDLTIFSLHLAGVSSILG AINFITTIINMKPPAMSQYQTPLFVWSVLITAVLLLLSLPVLAAGITMLLTDRNLNTTFFDPAGGGDPILYQHLFWFFGH PEVYILILPGFGMISHIVTYYSGKKEPFGYMGMVWAMMSIGFLGFIVWAHHMFTVGMDVDTRAYFTSATMIIAIPTGVKV FSWLATLHGGNIKWSPAMLWALGFIFLFTVGGLTGIVLANSSLDIVLHDTYYVVAHFHYVLSMGAVFAIMGGFVHWFPLF SGYTLNQAWAKIHFVIMFVGVNMTFFPQHFLGLSGMPRRYSDYPDAYTAWNTISSMGSFISLTAVMLMIFIIWEAFASKR EVSAVELTSTNLEWLHGCPPPYHTFEEPTYINLK O79876 7.1.1.9 Cytochrome c oxidase subunit 2 Sus scrofa 0.026203564 1 LEVO MAYPFQLGFQDATSPIMEELLHFHDHTLMIVFLISSLVLYIISLMLTTKLTHTSTMDAQEVETIWTILPAIILILIALPS LRILYMMDEINNPALTVKTMGHQWYWSYEYTDYEDLTFDSYMIPTSDLKPGEMRLLEVDNRVVLPMEMTIRMLVSSEDVL HSWAVPSLGLKTDAIPGRLNQTTLMSTRPGLYYGQCSEICGSNHSFMPIVLELVPLKYFEKWSTSMLT P50667 7.1.1.9 Cytochrome c oxidase subunit 3 Sus scrofa 0.029622168 1 LEVO THQTHAYHMVNPSPWPLTGALSALLMTSGLTMWFHFNSMLLLSLGLLTNTLTMYQWWRDIIRESTFQGHHTSVVQKGLRY GMILFIISEVLFFTGFFWAFYHSSLAPTPELGGCWPPTGIHPLNPLEVPLLNTSILLASGVSITWAHHSLMEGDRKHMIQ ALSITIALGVYFTLLQASEYYEAPFTISDGVYGSTFFVATGFHGLHVIIGSTFLAVCLLRQLKFHFTSNHHFGFEAAAWY WHFVDVVWLFLYVSIYWWGS Q35916 7.1.1.9 Cytochrome c oxidase subunit 4 Sus scrofa 0.016323619 1 LEVO EDYALPVYVDRRDYPLPDVAHVKNLSASQKALKEKEKASWSSLSMDEKVELYRLKFNESFAEMNRSTNEWKTIVGTALFF IGFTALLLIWEKHYVYGPIPHTFEEEWVAKQTKRMLDMKVAPIQGFSAKWDYDKNEWK A0A4X1T8J9 NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial Sus scrofa 0.005881824999999999 1 LEVO KFYIREPPHGSPDWLKVGLTLGTSVFLWIYLIKQHKEDVLEYKRRNGLE A1XQS6 NADH dehydrogenase [ubiquinone] 1 subunit C2 Sus scrofa 0.014196459 1 LEVO TMMSGRPGRVPLQFLPNEARSLPPPKLTDPRLVYMGFLGYCSGLIDNAIRRRPVVSAGLHRQLLYVTSFVFFGYYLLKRQ DYMYALRDHDMFAYVKSHPEDFPEKDKKTYGEILEEFHPVR A0A480JRW3 NADH-ubiquinone oxidoreductase chain 1 Sus scrofa 0.035667519999999994 1 LEVO MFMINILSLIIPILLAVAFLTLVERKVLGYMQLRKGPNVVGPYGLLQPIADALKLFTKEPLRPATSSISMFIIAPILALS LALTMWVPLPMPYPLINMNLGVLFMLAMSSLAVYSILWSGWASNSKYALIGALRAVAQTISYEVTLAIILLSVLLMNGSY TLSTLITTQEHIWMIFTSWPLAMMWFISTLAETNRAPFDLTEGESELVSGFNVEYAAGPFAMFFMAEYANIIMMNAFTAI LFLGASHDPHTPELYTINFVLKTLALTITFLWIRASYPRFRYDQLMHLLWKSFLPLTLALCMWHISLPIMTASIPPQS O79874 7.1.1.2 NADH-ubiquinone oxidoreductase chain 2 Sus scrofa 0.039077504 1 LEVO MNPIIYTTLIMTVMSGTMLVMISSHWLLIWIGFEMNLLAMIPVLMKNFNPRATEAATKYFLTQATASMMLMMAIIINLLY SGQWTITKMFNPVAMTMMTMALAMKLGLSPFHFWVPEVTQGISLQAGLLLLTWQKLAPLSVLCQISQSINPNLMLTMAML SILIGGWGGLNQTQLRKIMAYSSIAHMGWMTAVLPYNTTMTILNLLIYITMTLAMFMLLIHSSATTTLSLSHTWNKMPVI TSLMMVTLLSMGGLPPLSGFMPKWMIIQEMTKNESIIMPTLMAMTALLNLYFYMRLAYSSSLTMFPSTNNMKMKWQFEHT KQMKLLPTMIVLSTLVLPMTPALSSLN O79875 7.1.1.2 NADH-ubiquinone oxidoreductase chain 3 Sus scrofa 0.012998448 1 LEVO MNIMLTLLTNVTLASLLVLIAFWLPQLNAYSEKTSPYECGFDPMGSARLPFSMKFFLVAITFLLFDLEIALLLPLPWASQ TNNLKTMLTMALFLLILLAASLAYEWTQKGLEWAE O79880 7.1.1.2 NADH-ubiquinone oxidoreductase chain 4 Sus scrofa 0.051853355000000004 1 LEVO MLKIIIPTTMLLPMTWMSKHNMIWINATVHSLLISLISLSLLNQLGENSLNFSLTFFSDSLSAPLLVLTTWLLPLMLMAS QSHLSKETTTRKKLYITMLILLQLFLIMTFTATELILFYILFEATLVPTLIIITRWGNQTERLNAGLYFLFYTLAGSLPL LVALVYIQNTTGSLNFLIIHYWSHPLSNSWSNIFMWLACIMAFMVKMPLYGLHLWLPKAHVEAPIAGSMVLAAVLLKLGG YGMMRITTILNPLTNYMAYPFLMLSMWGMIMTSSICLRQTDLKSLIAYSSVSHMALVIVAIMIQTPWSFMGATALMIAHG LTSSMLFCLANTNYERVHSRTMILARGLQTLLPLMATWWLVASLTNLALPPSINLIGELFIITASFSWSNITIILMGMNM MITALYSLYMLITTQRGKYTHHINNIKPSFTRENALMALHILPLLLLTLNPKMILGPLY Q7IIB7 7.1.1.2 NADH-ubiquinone oxidoreductase chain 5 Sus scrofa 0.068267031 1 LEVO MNPFASLTLTTLTILTIPIMMSNSNIYKTNLYPNYVKTTVSYAFTLSLVPLLMFMHTGQEMIISNWHWMTLQTVELSLSF KMDYFSVMFIPVALFVTWSIMEFSMWYMHSDPFINRFFKYLLLFLITMMILVTANNLFQLFIGWEGVGIMSFLLIGWWHG RTDANTAALQAILYNRIGDIGFVLSMAWFLTHSNAWDLQQIFMLNNECPNMPLIGLLLAAAGKSAQFGLHPWLPSAMEGP TPVSALLHSSTMVVAGVFLLIRFYPLMETNKLVQTMTLCLGAITTLFTALCAITQNDIKKIVAFSTSSQLGLMMVTIGIN QPHLAFLHICMHAFFKAMLFMCSGSIIHSLNDEQDIRKMGGLYKAMPFTTTALIIGSLALTGMPYLTGFYSKDLIIEAVN MSYTNAWALLMTLIATSLTAAYSTRIIFFAFLGKPRFPPLVLINENNPLLINSIKRLLIGSIFAGFIISNNIPPMTVPNT TMPLYMKMTALIVTIMGFMLALELNNTTYYLKFKYPSQTYKFSNMLGYYPSIMHRLPTYHNLSMSQKSASSLLDLIWLET ILPKTTSFIQMKMSIMVSNQKGLIKLYFLSFLITIMISMTLFN Q71K68 7.1.1.2 NADH-ubiquinone oxidoreductase chain 6 Sus scrofa 0.018890135 1 LEVO TMYIAFILSTIFVIGFVGFSSKPSPIYGGLGLIVSGGVGCGIVLNFGGSFLGLMVFLIYLGGMLVVFGYTTAMATEMYPE VWVSNKTVFGAFVSGLMMEFCMVYYALKEEEVEIIFKFNGLGDWVIYDTGDSGFFSEEAMGIAALYSYGTWLVIVTGWSL LIGVVVIMEITRGN O79882 7.1.1.2 Cytochrome b-c1 complex subunit 2, mitochondrial Sus scrofa 0.044727214999999994 2 LEVO PQDLEFTRLPNGLVIASLENYAPASRIGLFIKAGSRYEDSNNLGTSHLLRLASSLTTKGASSFKITRGIEAVGGKLSVTS TRESMAYTVECLRDDIEILMEFLLNVTAAPEFRRWEVAALQSQLRIDKAVAFQNPQAQVLENLHAAAYRNALANSLYCPD YRIGKVTPDQLHYYVQNHFTSARMALIGLGVSHPVLKQVAERFLNMRGGLGLSGAKAKYRGGEIRDQNGDSLVHAALVAE SAATGSAEANAFSVLQHVLGAGPHVKRGSNATSSLYQAVAKGVHQPFDVSAFNASYSDSGLFGIYTISQAASAGDVIKSA YDQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSEGFLDEVGSQALVAGSYVQPSTVLQQIDSVADADVINAAKKFVSG RKSMAASGNLGHTPFVDEL F1RPD2 Cytochrome b-c1 complex subunit 1, mitochondrial Sus scrofa 0.049270133 2 LEVO TATYAQALQSVPETQVSQLDNGLRVASEQSSQPTCTVGVWIDAGSRYENEKNNGAGYFVEHLAFKGTKNRPGSALEKEVE SMGAHLNAYSTREHTAYYIKALSKDLPKAVELLADIVQNCSLEDSQIEKERDVILQELQENDSSMRDVVFDYLHATAFQG TPLAQSVEGPSENVRKLSRADLTEYVSQHYKAPRMVLAAAGGVEHRQLLDLAQKHFSSLSGTYVEDAVPAFTPCRFTGSE IRHRDDALPLAHVAIAVEGPGWANPDNVPLQVANAIIGHYDSTYGGGTHMSSTLASVAATRKLCQSFQTFNICYAETGLL GAHFVCDNMSIDDMMFFLQGQWMRLCTSATESEVVRGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIA EVDASVVREVCSKYFYDQCPAVAGLGPIEQLPDYNRIRSGMFWLRF A0A8D0PK14 Cytochrome b Sus scrofa 0.042840714999999994 2 LEVO MTNIRKSHPLMKIINNAFIDLPAPSNISSWWNFGSLLGICLILQILTGLFLAMHYTSDTTTAFSSVTHICRDVNYGWVIR YLHANGASMFFICLFIHVGRGLYYGSYMFLETWNIGVVLLFTVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDL VEWIWGGFSVDKATLTRFFAFHFILPFIITALAAVHLLFLHETGSNNPTGISSDMDKIPFHPYYTIKDILGALFMMLILL ILVLFSPDLLGDPDNYTPANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVLALVASILILILMPMLHTSKQRSMMFRPL SQCLFWMLVADLITLTWIGGQPVEHPFIIIGQLASILYFLIILVLMPITSIIENNLLKW Q1HBG9 Cytochrome c1, heme protein, mitochondrial Sus scrofa 0.027373356999999997 2 LEVO TDLELHAPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCSSCHSMDYVAYRHLVGVCYTEEEAKALAEEVEVQDGPNEDGEM FMRPGKLSDYFPKPYPNPEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAM APPIYNEVLEFDDGTPATMSQVAKDVCTFLRWASEPEHDHRKRMGLKMLMMMGLLLPLVYAMKRHKWSVLKSRKLAYRPP K A0A4X1UNI7 Cytochrome b-c1 complex subunit Rieske, mitochondrial Sus scrofa 0.029492599999999997 3 LEVO MLSVASRSGPFAPVLSATSRGVAGALRPLVQAALPATSESPVLDAKRSFLCRESLSGQAAGRPLVASVGLNVPASVRYSH TDIRVPDFSDYRRAEVLDSTKSSKESSDARKGFSYLITATTTVGVAYAAKNAVSQFVSSMSASADVLAMSKIEIKLSDIP EGKNMAFKWRGKPLFVRHRTKKEIDQEAAVEVSQLRDPQHDLERVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHG SHYDASGRIRKGPAPLNLEVPTYEFTSDDLVIVG A0A4X1TWD8 7.1.1.8 Cytochrome b-c1 complex subunit 6 Sus scrofa 0.007933944 2 LEVO ELVDPLTTVREQCEQIEKCIKARERLELCDQRVSSRSQTEEDCTEELFDFLHARDHCVAHKLFNSLK F1S3W0 Cytochrome b-c1 complex subunit 7 Sus scrofa 0.012644447 2 LEVO SKWLEGIRKWYYNAAGFNKLGLMRDDTIYEDDDVKEAIRRLPENLYNDRVFRIKRALDLTMRQQILPKEQWTKYEEDKFY LEPYLKEVIRERKEREEWAKK A0A4X1U2M7 Cytochrome b-c1 complex subunit 8 Sus scrofa 0.009408876 2 LEVO GREFGHLTRMRHVITYSLSPFEQRAFPHYFTKGIPNVLRRTRACILRVAPPFVVFYLVYTWGTQEFEKSKRKNPAAYEN A0A4X1UYT9 Complex III subunit 9 Sus scrofa 0.00715222 2 LEVO AAPTLTARLYSLLFRRTSTFALTIAVGALFFERAFDQGADAIYEHINQGKLWKHIKHKYENK Q2EN79 Cytochrome b-c1 complex subunit 10 Sus scrofa 0.006058093 2 LEVO MLSRFLGPRYRELARNWIPTASMWGAVGAVGLVWATDWRLILDWVPYINGKF A0A4X1VTU3 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Sus scrofa 0.07568367999999999 1 LEVO NLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKS KKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEI AGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGE VMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTQPMIRNEKGLLTYTTWEDALSRVAGMLQSFQGNDVAAIAGGLVDAE ALVALKDLLNRVDSDSLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVILLVGTNPRFEAPLFNARIRKSWLHNDLKV ALIGSPVDLTYRYDHLGDSPKILQDIASGNHPFSQILKEAKKPMVVLGSSALQRSDGTAILAAVSNIAQNIRLSSGVTGD WKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDLPKDCFIIYQGHHGDVGAPMADVILPGAAY TEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRSRLEEVSPNLVRYDDVEGANYFQQAN ELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGI A0A4X1V1H9 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Sus scrofa 0.049234323999999996 1 LEVO ARQWQPDVEWAEQFGGAVMYPTKETAHWKPPPWNDVDPPKDTLVSNLTLNFGPQHPAAHGVLRLVMELSGEMVRKCDPHI GLLH(2MR)GTEKLIEYKTYLQALPYFDRLDYVSMMCNEQAYSLAVEKLLNIQPPPRAQWIRVLFGEITRLLNHIMAVTT HALDIGAMTPFFWMFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLLDDIYEFSKNFSFRIDELEEMLTNNRIWR NRTVDIGVVTAEDALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPIGSRGDCYDRYLCRVEEMRQSLRIISQCLN KMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGF AHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR F1S1A8 Complex I-30kD Sus scrofa 0.02452173 1 LEVO TRPTIRPRNDVVHKQLSAFGQYVAEILPKYVQQVQVSCFNELEIFIHPDGVIPVLTFLRDHTNAQFKSLADLTAVDVPTR QNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESSVTVYKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDF PLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPAYRQPPE A0A286ZNN4 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Sus scrofa 0.014314259999999999 1 LEVO LIAVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWSSTADPLSNLVLTFSTK EDAVAFAEKNGWSFDVEERKVPKPKSKSYGANFSWNKRTRVSTK A0A4X1SN37 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 Sus scrofa 0.012375394999999999 1 LEVO PFFDVQKRLGLDLDRWMTIQSAEQPHKIPGRCHAFEKEWIECAHGIGGIRAEKECKIEFDDFVECLLRQKTMKRLSAIKR QRDKLIKEGKYTPPPHHLGKEDPRP F1SV23 NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Sus scrofa 0.010567635 1 LEVO GVRTSPTGEKVTHTGQAYDDGDYRRVRFSDRQKEVNENFAIDLIAEQPVSEVGSRVISCDGGGGALGHPRVYINLDKETK TGTCGYCGLQFRQPHH F1S031 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Sus scrofa 0.017874953 1 LEVO SRGEYVVAKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKV YDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQRKIKREKRLRIWYRR A0A8D1TL60 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Sus scrofa 0.020207957 1 LEVO TYKFVNMREPSMDMKSVTDRAAQTLLWTELVRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIAC KLCEAVCPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDK WEAEIAANIQADYLYR A0A287BDC0 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Sus scrofa 0.04723578899999999 1 LEVO TSFGSLKDEDRIFTNLYGRHDWRLKGAQSRGDWYKTKEILLKGPDWILGEVKTSGLRGRGGAGFPTGLKWSFMNKPSDGR PKYLVVNADEGEPGTCKDREIIRHDPHKLVEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGS GYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGAWFASFGRER NSGTKLFNISGHVNHPCTVEEEMSVPLKELIEKHAGGVIGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALVQAQTGL GTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVKGDARPAEIDSLWEISKQIEGHTICALGD GAAWPVQGLIRHFRPELEERMQQFALQHQAR A0A4X1SZP7 7.1.1.2 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Sus scrofa 0.023826335999999997 1 LEVO GAGGALFVHRDTPENNPDTPFDFTPENYKRIEAIVKNYPEGHKAAAVLPVLDLAQRQNGWLPISAMNKVAEILQVPPMRV YEVATFYTMYNRKPVGKYHIQVCTTTPCMLRNSDSILEAIQKKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDNY YEDLTPKDIEEIIDELKAGKIPKPGPRSGRFSCEPAGGLTSLTEPPKGPGFGVQAGL A0A287BG40 NADH:ubiquinone oxidoreductase subunit V3 Sus scrofa 0.005046508999999999 1 LEVO DNSTYRNLQHHEYSTYTFLDLNVELSKFRMPQPSSGRQSPRH A0A287BEK4 CARDIOLIPIN 0.0014640429999999997 21 CDL ZINC ION 6.5409e-05 2 ZN 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE 0.0007901449999999999 15 PC1 (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL 0.0007671319999999999 10 PLX 1,2-Distearoyl-sn-glycerophosphoethanolamine 0.000748065 12 3PE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine 0.000744034 16 PEE NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE 0.000745421 1 NDP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate 0.000568704 2 ZMP ADENOSINE-5'-DIPHOSPHATE 0.000427201 1 ADP HEME-A 0.000852837 2 HEA COPPER (II) ION 6.354599999999999e-05 3 CU MAGNESIUM ION 2.4305e-05 2 MG PROTOPORPHYRIN IX CONTAINING FE 0.000616487 4 HEM HEME C 0.0006185030000000001 2 HEC FE2/S2 (INORGANIC) CLUSTER 0.00017581999999999998 4 FES IRON/SULFUR CLUSTER 0.00035163999999999996 6 SF4 Metformin 0.00012916399999999997 1 MF8 UBIQUINONE-10 0.0008633429999999999 1 U10 FLAVIN MONONUCLEOTIDE 0.00045634399999999997 1 FMN singleParticle particle 0.25 7.4 20.0 Tris Trimethylsilyl 50.0 NaCl Sodium chloride 0.002 PMSF Phenylmethylsulfonyl 0.1 GDN Glyco-diosgenin

SEC buffer (20 mM Tris pH 7.4, 50 mM NaCl, 0.002% PMSF, 0.1% GDN (w/v))

Quantifoil R1.2/1.3 COPPER 300 CARBON CONTINUOUS 2.0 GLOW DISCHARGE 15 AIR 0.039

15mA

ETHANE 100 277.15 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.6 1.8 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON IV (4k x 4k) 4096 4096 1 7.8 51.9 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 3D ab-inito model reconstruction in cryosparc 2.86 FSC 0.143 CUT-OFF cryoSPARC v4.4.1 320129 COMMON LINE cryoSPARC v4.4.1 COMMON LINE cryoSPARC v4.4.1 cryoSPARC v4.4.1 emd_60424.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 Z Y X -34.575992999999997 46.884169999999997 -0.00009792044 1.005766 1.2 1.2 1.2 0.05 AUTHOR ::::EMDATABANK.org::::EMD-60424:::: RIGID BODY FIT REAL emd_60424_msk_1.map emd_60424_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 X Y Z -1.3183854 5.1432886 0.0014107736 0.11903494 1.2 1.2 1.2 AUTHOR ::::EMDATABANK.org::::EMD-60424:::: emd_60424_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 X Y Z -1.1431134 5.1176043 0.0014426549 0.11316264 1.2 1.2 1.2 AUTHOR ::::EMDATABANK.org::::EMD-60424::::