2025-12-17 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBj 2024-06-05 2025-12-17 2025-12-17 2025-12-17 Other government ZJU100 Young Professor China Teng F He ZX Hu YQ Xu CY Guo RY Zhou L Metformin, electron transport chain, Respirasome, mammalia, MEMBRANE PROTEIN Teng F He ZX Yang YQ Guo RY Hu YQ Xu CY Zhou RH Zhang X Zhang S Zhou L To Be Published 0353 EMD-60371 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO)

EMD-60372 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO), complex I peripheral arm focused

EMD-60373 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO), complex I proximal membrane arm focused

EMD-60374 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO), complex I proximal membrane arm focused

8zsr FULLOVERLAP EMDB EMD-60371 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO)

EMDB EMD-60372 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO), complex I peripheral arm focused

EMDB EMD-60373 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO), complex I proximal membrane arm focused

EMDB EMD-60374 other EM volume

Cryo-EM map of respirasome open state bound by proguanil (SC-ProgO), complex I proximal membrane arm focused

EMDB EMD-60425 associated EM volume

Complex I form respirasome open state bound by proguanil (SC-ProgO)

Respirasome open state inhibited by proguanil Respirasome open state inhibited by proguanil 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Sus scrofa NADH-ubiquinone oxidoreductase chain 4L Sus scrofa 0.010827253 1 LEVO MPLVYMNIIMAFAIALAGLLMYRSHLMSSLLCLEGMMLSLFIMSTLIILNTHFTLANMMPIILLVFAACEAALGLSLLVM VSNTYGTDYVQNLNLLQC P56632 7.1.1.2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Sus scrofa 0.008034373 1 LEVO MWFEILPGIAVMAACLFIPGMATAHIHKFTNGGKEKRVAHFSYQWNLMERDRCISGVNRYHVTKGLENID K7GR43 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Sus scrofa 0.009770203 1 LEVO LGLREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQEKNVSLNNFSADQVTRTLEN VLSGK F1RGE3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 Sus scrofa 0.009094371999999998 1 LEVO AGRIASFLKNAWAKEPVLVASFAIGGLAIILPSLSPYTNYAIRINRATPYNYPVPLRDDGNMPDVPSHPQDPQGPSLEWL KNL A0A4X1VPG4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Sus scrofa 0.012949105999999998 1 LEVO LKKTTGLVGLAVCETPHERLKILYTKILDVLGQIPKNAAYRKYTEQITNEKLGMVKAEPDVKKLEEQLQGGQIEEVILQA ENELSLARKMLRWKPWEPLVEEPPANQWKWPI A0A8D1IJ52 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Sus scrofa 0.013755924000000001 1 LEVO TSVKPIFSRDMNEAKRRVRELYRAWYREVPNTVHLFQLDISVKQGRDKVREMFMKNAHVTDPRVVDLLVIKGKMELEETI NVWKQRTHIMRFFHETEAPRPTDFLSKFYVGHDP F1SJP6 Complex I-B14.5a Sus scrofa 0.012517394 1 LEVO ASATRVIQLLRNWASGRDLQAKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRREAMPPSIVMSSQKVLASGKP AESSAVAETEKKAVTPAPPIKRWELSKDQPYL A0A4X1VLA2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Sus scrofa 0.019932898 1 LEVO PGIVELPTLEDLKVQEVKVSSSVLKAAAHHYGAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNQCALDFFRQIKRHCAEP FTEYWTCIDYSGLQLFRHCRKQQAKFDECVLDKLGWVRPDLGELSKVTKVKTDRPLPENPYHSRARPEPNPEAEGDLKPA KHGSRLFFWTM F1SLR1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Sus scrofa 0.03884089499999999 1 LEVO LHHALIPHGKGGRSSVSGIVATVFGATGFLGRYVVNHLGRMGSQVIVPYRCEPYDTMHLRPMGDLGQIIFMEWNGKDKDS IRKVVEHSNVVINLVGREWETKNFDFEDVFVKIPHAIAQVSKEAGVEKLIHISHLNADIKSPSRYLRSKAVGEKEVRAAF PEATIIKPSDIFGREDRFLNYFASMRWFGGVPLISLGKETVKQPVYIVDVSKGIINAIKDPDAKGKTFAFVGPNRYLLFD LVQYIFAVAYRPFLPYPLPHFAYRWVGRLFEVSPFEPWTTRDKVERVHMSDMTLPHLPGLEDLGIQATPLELKAIEVLRR HRTYRWLTSEMEDVKPAKTVN F1SL07 Acyl carrier protein Sus scrofa 0.010046488 2 LEVO DAPPLTLEAIKDRVLYVLKLYDKIDPEKLSVNSHFMKDLGLDSLDQVEIIMAMEDEFGFEIPDIDAEKLMCPQEIVDYIA DKKDVYE A0A287AAR5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Sus scrofa 0.036949875 1 LEVO RLQYGPLAFVLGERTTRKLTETSKVITVDGNICSGKGRLAREIAEKLGLRHFPEAGIHYADSTTGDGKPLDVQLSGNCSL EKFYDDPKSNDGNSYRLQSWLYASRLLQYADALEHLLSTGQGVVLERSIYSDFVFLEAMYRQGFIRKQCVEHYNEVKKVT ACEYLPPHVVVYVDVPVPEIQSRIQKKGNPHEMKITAAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAEDAEKVVEDIE YLKCDKGPWPDQDDRTFHRLRMLVQNKLEVLNYTTIPVYLPEITIGAHQSDRVFQKFTELPGRKYSPGYNEDVGDKWIWL K F1SIS9 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 Sus scrofa 0.014555697999999999 1 LEVO AKTLLHKYSDIPEGTECHRKAYASTSIGGATGLIVSAYSIALKPPASFLEGVARTGRYTFTSAAIGAIFGLTSCISAQVR EKPDDPLNYFIGGCAGGLTLGARTRSYGIGAAACAYMGLTAALVKMGQLEGWQVFAEPKV I3LGM4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Sus scrofa 0.017031243999999997 1 LEVO ELVQVLRRGLQQVSGHGGLRGYLRVLFRANDVRVGTLVGEDKYGNKYYEDNKQFFGRHRWVIYTTEMNGRDTFWDVDGSM VPPEWHRWLHCMTDDPPTTKPPTARKYIWTNHKFNVSGTPQQYVPYSTTRKKIQEWVPPSTPYK F1SQP4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Sus scrofa 0.01670934 1 LEVO ASKVKQDMPPPGGYGPIDYKRNLPRRGLSGYSMFAVGIGTLLFGYWSMMKWNRERRRLQIEDFEARIALMPLFQAEKDRR VLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVTPMMGELYGLRTNEEILSATYGFIWYT A0A8D1NRT6 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 Sus scrofa 0.006781886 1 LEVO NVLQIVRDHWVHILVPVGFVFGCYLDRRSDEKLTAFRNKSLLFKRELRPNEEVTWK A0A8D2CGH8 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial Sus scrofa 0.008201093 1 LEVO VHIEPRYRQFPQLTRSQLIQAEFFSATMWFWILWRFWHDSDAVLGHFPYPDPSQWTDEELGILPDDE A0A5G2QW32 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 Sus scrofa 0.009042211999999999 1 LEVO SKMELPDYKQWKIEGTPLETVQEKLAARGLRDPWGRNEAWRYSGGFANNVSFVGALLKGFKWGFAAFVVAVGAEYYLESQ A0A4X1VD84 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Sus scrofa 0.014986153 1 LEVO SFPKYKPSRLATLPATLDPAEYDISPETRKAQAERLAIRSRLKREYLLQYNDPNRLGVIEDPALIRWTYARSANIYPNFR PTPKTSLLGALFGIGPLFFWYYVFKTDRDKKEKLIQEGKLDQTFNISY A0A4X1T6M0 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial Sus scrofa 0.016281898 1 LEVO KRLFIIKPSGFYDRRFLKLMRFYILLTGIPVAIGITLVNVFIGEAELADIPEGYVPEHWEYFKHPISRWIARTFYDGPEK NYEKTMAILQIEAEKAELRLKELEVRRLMRARGDGPWYQYPTIDKALIDHSPKTTPDN F1SGC6 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Sus scrofa 0.015333832 1 LEVO SGYTPDEKLRLQQLRELRRRWLKDQELSPREPVLPPRRVWPMEQFWNKFLQDGAPWKNVIYKTYRHSIFAVTHVLIPVWI IHYYLKYHVTAKPYTVVERKPRIFPGDTILETGEVIPLMKEFPDQH A0A480KM09 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Sus scrofa 0.015307553 1 LEVO MGAHLARRYLGDASKEPDPLRMPTFPPDYGFPERKEREMVATQQEMNDAQLMLQQRDYCAHYLIQLLKCKRDSFPNFLAC KHEQHDWDYCEHLDYVKRMKEFERERRLLQRKKRREQREAEMARG F1SCH1 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Sus scrofa 0.018608805 1 LEVO HVTKDMFPGPYPKTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPDRSQQERDPWYDWDHPDLRLNWGEPIHWDLD MYIRNRVDTSPTPVSWNTMCKHLFGFVAFMLFMFWVGEIYPSYQPVGPKQYPYNDLYLERGGDPTKEPEPVVHYEI F1RWL7 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Sus scrofa 0.021733711 1 LEVO AFSAPAAYLTHQQKVLRLYKRALRHLESWCVHRDKYRYFACLMRARFDEHKNEKDMVKATQLLRQAEEEFWYGQHPQPYI FPESPGGTSYERYECYKVPEWCLDDWHPSEKAMYPDYFAKREQWKRLRRESWEREVKQLQEETPPGGPRTEALPPARKEG DLPPLWWHIVTRPRERPM K7GSE5 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 Sus scrofa 0.020944768 1 LEVO MPDSWDKDVYPEPPRRTPAPAPQTSLPNPVTYLTKIFDLLVDRPVTLAREFIEQQHAKNRYYYYHREFRRVPDITECEEK DILCMFEAEMQWRRDYKVDQEIVNIIQERLKACQQREGESYRQNCAKELEQFTQVSKAFQDRYSDLGAHYSARKCLAKQK QRMLAERKAAKEAAAA I3LDC3 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial Sus scrofa 0.012113493 1 LEVO IRLQEDPDPEDENLYEKNPDSHGYDKDPIVDLWNMRVVFFFGFSIVLVLGSTFVAYLPDYRMQEWARREAERLVKYREAN GLPLMESNCFDPNKIQLPEDED A0A8D1EYI7 NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial Sus scrofa 0.005881824999999999 1 LEVO KFYIREPPHGSPDWLKVGLTLGTSVFLWIYLIKQHKEDVLEYKRRNGLE A1XQS6 NADH dehydrogenase [ubiquinone] 1 subunit C2 Sus scrofa 0.014196459 1 LEVO TMMSGRPGRVPLQFLPNEARSLPPPKLTDPRLVYMGFLGYCSGLIDNAIRRRPVVSAGLHRQLLYVTSFVFFGYYLLKRQ DYMYALRDHDMFAYVKSHPEDFPEKDKKTYGEILEEFHPVR A0A480JRW3 NADH-ubiquinone oxidoreductase chain 1 Sus scrofa 0.035667519999999994 1 LEVO MFMINILSLIIPILLAVAFLTLVERKVLGYMQLRKGPNVVGPYGLLQPIADALKLFTKEPLRPATSSISMFIIAPILALS LALTMWVPLPMPYPLINMNLGVLFMLAMSSLAVYSILWSGWASNSKYALIGALRAVAQTISYEVTLAIILLSVLLMNGSY TLSTLITTQEHIWMIFTSWPLAMMWFISTLAETNRAPFDLTEGESELVSGFNVEYAAGPFAMFFMAEYANIIMMNAFTAI LFLGASHDPHTPELYTINFVLKTLALTITFLWIRASYPRFRYDQLMHLLWKSFLPLTLALCMWHISLPIMTASIPPQS O79874 7.1.1.2 NADH-ubiquinone oxidoreductase chain 2 Sus scrofa 0.039077504 1 LEVO MNPIIYTTLIMTVMSGTMLVMISSHWLLIWIGFEMNLLAMIPVLMKNFNPRATEAATKYFLTQATASMMLMMAIIINLLY SGQWTITKMFNPVAMTMMTMALAMKLGLSPFHFWVPEVTQGISLQAGLLLLTWQKLAPLSVLCQISQSINPNLMLTMAML SILIGGWGGLNQTQLRKIMAYSSIAHMGWMTAVLPYNTTMTILNLLIYITMTLAMFMLLIHSSATTTLSLSHTWNKMPVI TSLMMVTLLSMGGLPPLSGFMPKWMIIQEMTKNESIIMPTLMAMTALLNLYFYMRLAYSSSLTMFPSTNNMKMKWQFEHT KQMKLLPTMIVLSTLVLPMTPALSSLN O79875 7.1.1.2 NADH-ubiquinone oxidoreductase chain 3 Sus scrofa 0.012998448 1 LEVO MNIMLTLLTNVTLASLLVLIAFWLPQLNAYSEKTSPYECGFDPMGSARLPFSMKFFLVAITFLLFDLEIALLLPLPWASQ TNNLKTMLTMALFLLILLAASLAYEWTQKGLEWAE O79880 7.1.1.2 NADH-ubiquinone oxidoreductase chain 4 Sus scrofa 0.051853355000000004 1 LEVO MLKIIIPTTMLLPMTWMSKHNMIWINATVHSLLISLISLSLLNQLGENSLNFSLTFFSDSLSAPLLVLTTWLLPLMLMAS QSHLSKETTTRKKLYITMLILLQLFLIMTFTATELILFYILFEATLVPTLIIITRWGNQTERLNAGLYFLFYTLAGSLPL LVALVYIQNTTGSLNFLIIHYWSHPLSNSWSNIFMWLACIMAFMVKMPLYGLHLWLPKAHVEAPIAGSMVLAAVLLKLGG YGMMRITTILNPLTNYMAYPFLMLSMWGMIMTSSICLRQTDLKSLIAYSSVSHMALVIVAIMIQTPWSFMGATALMIAHG LTSSMLFCLANTNYERVHSRTMILARGLQTLLPLMATWWLVASLTNLALPPSINLIGELFIITASFSWSNITIILMGMNM MITALYSLYMLITTQRGKYTHHINNIKPSFTRENALMALHILPLLLLTLNPKMILGPLY Q7IIB7 7.1.1.2 NADH-ubiquinone oxidoreductase chain 5 Sus scrofa 0.068267031 1 LEVO MNPFASLTLTTLTILTIPIMMSNSNIYKTNLYPNYVKTTVSYAFTLSLVPLLMFMHTGQEMIISNWHWMTLQTVELSLSF KMDYFSVMFIPVALFVTWSIMEFSMWYMHSDPFINRFFKYLLLFLITMMILVTANNLFQLFIGWEGVGIMSFLLIGWWHG RTDANTAALQAILYNRIGDIGFVLSMAWFLTHSNAWDLQQIFMLNNECPNMPLIGLLLAAAGKSAQFGLHPWLPSAMEGP TPVSALLHSSTMVVAGVFLLIRFYPLMETNKLVQTMTLCLGAITTLFTALCAITQNDIKKIVAFSTSSQLGLMMVTIGIN QPHLAFLHICMHAFFKAMLFMCSGSIIHSLNDEQDIRKMGGLYKAMPFTTTALIIGSLALTGMPYLTGFYSKDLIIEAVN MSYTNAWALLMTLIATSLTAAYSTRIIFFAFLGKPRFPPLVLINENNPLLINSIKRLLIGSIFAGFIISNNIPPMTVPNT TMPLYMKMTALIVTIMGFMLALELNNTTYYLKFKYPSQTYKFSNMLGYYPSIMHRLPTYHNLSMSQKSASSLLDLIWLET ILPKTTSFIQMKMSIMVSNQKGLIKLYFLSFLITIMISMTLFN Q71K68 7.1.1.2 NADH-ubiquinone oxidoreductase chain 6 Sus scrofa 0.018890135 1 LEVO TMYIAFILSTIFVIGFVGFSSKPSPIYGGLGLIVSGGVGCGIVLNFGGSFLGLMVFLIYLGGMLVVFGYTTAMATEMYPE VWVSNKTVFGAFVSGLMMEFCMVYYALKEEEVEIIFKFNGLGDWVIYDTGDSGFFSEEAMGIAALYSYGTWLVIVTGWSL LIGVVVIMEITRGN O79882 7.1.1.2 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Sus scrofa 0.07568367999999999 1 LEVO NLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKS KKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEI AGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGE VMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTQPMIRNEKGLLTYTTWEDALSRVAGMLQSFQGNDVAAIAGGLVDAE ALVALKDLLNRVDSDSLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVILLVGTNPRFEAPLFNARIRKSWLHNDLKV ALIGSPVDLTYRYDHLGDSPKILQDIASGNHPFSQILKEAKKPMVVLGSSALQRSDGTAILAAVSNIAQNIRLSSGVTGD WKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDLPKDCFIIYQGHHGDVGAPMADVILPGAAY TEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRSRLEEVSPNLVRYDDVEGANYFQQAN ELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGI A0A4X1V1H9 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Sus scrofa 0.049234323999999996 1 LEVO ARQWQPDVEWAEQFGGAVMYPTKETAHWKPPPWNDVDPPKDTLVSNLTLNFGPQHPAAHGVLRLVMELSGEMVRKCDPHI GLLH(2MR)GTEKLIEYKTYLQALPYFDRLDYVSMMCNEQAYSLAVEKLLNIQPPPRAQWIRVLFGEITRLLNHIMAVTT HALDIGAMTPFFWMFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLLDDIYEFSKNFSFRIDELEEMLTNNRIWR NRTVDIGVVTAEDALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPIGSRGDCYDRYLCRVEEMRQSLRIISQCLN KMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGF AHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR F1S1A8 Complex I-30kD Sus scrofa 0.02452173 1 LEVO TRPTIRPRNDVVHKQLSAFGQYVAEILPKYVQQVQVSCFNELEIFIHPDGVIPVLTFLRDHTNAQFKSLADLTAVDVPTR QNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESSVTVYKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDF PLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPAYRQPPE A0A286ZNN4 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Sus scrofa 0.014314259999999999 1 LEVO LIAVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWSSTADPLSNLVLTFSTK EDAVAFAEKNGWSFDVEERKVPKPKSKSYGANFSWNKRTRVSTK A0A4X1SN37 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 Sus scrofa 0.012375394999999999 1 LEVO PFFDVQKRLGLDLDRWMTIQSAEQPHKIPGRCHAFEKEWIECAHGIGGIRAEKECKIEFDDFVECLLRQKTMKRLSAIKR QRDKLIKEGKYTPPPHHLGKEDPRP F1SV23 NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Sus scrofa 0.010567635 1 LEVO GVRTSPTGEKVTHTGQAYDDGDYRRVRFSDRQKEVNENFAIDLIAEQPVSEVGSRVISCDGGGGALGHPRVYINLDKETK TGTCGYCGLQFRQPHH F1S031 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Sus scrofa 0.017874953 1 LEVO SRGEYVVAKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKV YDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQRKIKREKRLRIWYRR A0A8D1TL60 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Sus scrofa 0.020207957 1 LEVO TYKFVNMREPSMDMKSVTDRAAQTLLWTELVRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIAC KLCEAVCPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDK WEAEIAANIQADYLYR A0A287BDC0 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Sus scrofa 0.04723578899999999 1 LEVO TSFGSLKDEDRIFTNLYGRHDWRLKGAQSRGDWYKTKEILLKGPDWILGEVKTSGLRGRGGAGFPTGLKWSFMNKPSDGR PKYLVVNADEGEPGTCKDREIIRHDPHKLVEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGS GYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGAWFASFGRER NSGTKLFNISGHVNHPCTVEEEMSVPLKELIEKHAGGVIGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALVQAQTGL GTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVKGDARPAEIDSLWEISKQIEGHTICALGD GAAWPVQGLIRHFRPELEERMQQFALQHQAR A0A4X1SZP7 7.1.1.2 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Sus scrofa 0.023826335999999997 1 LEVO GAGGALFVHRDTPENNPDTPFDFTPENYKRIEAIVKNYPEGHKAAAVLPVLDLAQRQNGWLPISAMNKVAEILQVPPMRV YEVATFYTMYNRKPVGKYHIQVCTTTPCMLRNSDSILEAIQKKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDNY YEDLTPKDIEEIIDELKAGKIPKPGPRSGRFSCEPAGGLTSLTEPPKGPGFGVQAGL A0A287BG40 NADH:ubiquinone oxidoreductase subunit V3 Sus scrofa 0.005046508999999999 1 LEVO DNSTYRNLQHHEYSTYTFLDLNVELSKFRMPQPSSGRQSPRH A0A287BEK4 CARDIOLIPIN 0.0014640429999999997 12 CDL 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine 0.000744034 10 PEE NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE 0.000745421 1 NDP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate 0.000568704 2 ZMP ADENOSINE-5'-DIPHOSPHATE 0.000427201 1 ADP 1,2-Distearoyl-sn-glycerophosphoethanolamine 0.000748065 3 3PE 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE 0.0007901449999999999 6 PC1 (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL 0.0007671319999999999 7 PLX N-(4-chlorophenyl)-N'-(propan-2-yl)triimidodicarbonic diamide 0.000253731 2 XEW IRON/SULFUR CLUSTER 0.00035163999999999996 6 SF4 FE2/S2 (INORGANIC) CLUSTER 0.00017581999999999998 2 FES MAGNESIUM ION 2.4305e-05 1 MG ZINC ION 6.5409e-05 1 ZN FLAVIN MONONUCLEOTIDE 0.00045634399999999997 1 FMN singleParticle particle 0.25 7.4 20.0 Tris Trimethylsilyl 50.0 NaCl Sodium chloride 0.002 PMSF Phenylmethylsulfonyl 0.1 GDN Glyco-diosgenin

SEC buffer (20 mM Tris pH 7.4, 50 mM NaCl, 0.002% PMSF, 0.1% GDN (w/v))

Quantifoil R1.2/1.3 COPPER 300 CARBON CONTINUOUS 2.0 ETHANE 100 277.15 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.6 1.8 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON IV (4k x 4k) 4096 4096 1 7.8 51.9 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 3D ab-inito model reconstruction in cryosparc 3.1 FSC 0.143 CUT-OFF cryoSPARC v4.4.1 133663 COMMON LINE cryoSPARC v4.4.1 COMMON LINE cryoSPARC v4.4.1 cryoSPARC v4.4.1 RIGID BODY FIT REAL emd_60425_msk_1.map emd_60425_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 X Y Z -1.0897912 5.565787 0.004354361 0.15373756 1.2 1.2 1.2 AUTHOR ::::EMDATABANK.org::::EMD-60425:::: emd_60425_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 576.0 576.0 576.0 90.0 90.0 90.0 X Y Z -1.0489187 5.8797626 0.0043378724 0.15446623 1.2 1.2 1.2 AUTHOR ::::EMDATABANK.org::::EMD-60425::::