2026-01-21 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBj 2024-07-11 2026-01-21 2026-01-21 2026-01-21 National Research Foundation (NRF, Korea) RS-2023-00217798 Korea, Republic Of Other private SUHF-18010097 Korea, Republic Of Other private Korea, Republic Of Consensus map of Cx36/GJD2 gap junction intercellular channel in soybean polar lipid nanodiscs, treated with a 20-fold molar excess of carbenoxolone (including D6 and D1 symmetry maps) Jang HS Gap junction, Connexin36, Inhibitor, Carbenoxolone, MEMBRANE PROTEIN Lee CW Jang HS Woo JS Structural insights into the inhibition of GJCh by carbenoxolone To Be Published 0353 EMD-60761 other EM volume EMD-60777 other EM volume EMDB EMD-60761 other EM volume EMDB EMD-60775 associated EM volume EMDB EMD-60777 other EM volume Connexin36 Connexin36 0 1
BRIL-fused connexin36 gap junction intercellular channel is reconstituted into lipid nanodiscs with soybean polar lipids and MSP1E1. Carbenoxolone dissolved in 10% (v/v) DMSO is treated to the sample with 20-fold molar excess to Cx36 protomer.
Homo sapiens Connexin36 Homo sapiens
The human Cx36-BRIL proteins were expressed as fusion constructs with a human rhinovirus (HRV) 3C cleavage site, an enhanced yellow fluorescence protein (eYFP) tag, a 10xHis-tag and a rho-1D4 epitope tag (8 amino acid sequence of TETSQVAPA) at its C-terminus.
Homo sapiens LEVO MGEWTILERLLEAAVQQHST MIGRILLTVVVIFRILIVAI VGETVYDDEQTMFVCNTLQP GCNQACYDRAFPISHIRYWV FQIIMVCTPSLCFITYSVHQ SAKQRERRADLEDNWETLND NLKVIEKADNAAQVKDALTK MRAAALDAQKATPPKLEDKS PDSPEMKDFRHGFDILVGQI DDALKLANEGKVKEAQAAAE QLKTTRNAYIQKYLKLRRQE GISRFYIIQVVFRNALEIGF LVGQYFLYGFSVPGLYECNR YPCIKEVECYVSRPTEKTVF LVFMFAVSGICVVLNLAELN HLGWRKIKLAVRGAQAKRKS IYEIRNKDLPRVSVPNFGRT QSSDSAYVSRGDMLEVLFQ Q9UKL4 singleParticle particle 2.54 7.5 20.0 C8H18N2O4S HEPES 150.0 KCl Potassium chloride 2.0 C2H6OS beta-mercaptoethanol
20mM HEPES(pH 7.5), 150mM KCl, 2mM beta-mercaptoethanol
 Quantifoil R1.2/1.3 COPPER 200 CARBON HOLEY GLOW DISCHARGE OTHER ETHANE 95 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 0.5 2.0 120000.0 NITROGEN GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 5096 4092 6001 6.76 60.0 1 1648256 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION D6 2.37 FSC 0.143 CUT-OFF cryoSPARC 447636 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC emd_60775.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 307.19998 307.19998 307.19998 90.0 90.0 90.0 X Y Z -0.10324981 0.24752237 -0.00014779334 0.007118858 0.64 0.64 0.64 0.05 AUTHOR primary map refined with D6 symmetry emd_60775_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 307.19998 307.19998 307.19998 90.0 90.0 90.0 X Y Z -0.11793426 0.26016486 -0.00015319136 0.00733302 0.64 0.64 0.64 AUTHOR the map refined with D1 symmetry, using same particles to D6 map emd_60775_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 307.19998 307.19998 307.19998 90.0 90.0 90.0 X Y Z -0.1613849 0.2790702 0.00011500264 0.018854544 0.64 0.64 0.64 AUTHOR half-map of primary map emd_60775_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 307.19998 307.19998 307.19998 90.0 90.0 90.0 X Y Z -0.16621144 0.27926567 0.00011444276 0.018852271 0.64 0.64 0.64 AUTHOR half-map of primary map