Hemichannel sub-structure of Cx36/GJD2 gap junction intercellular channel (FN conformation) in soybean polar lipid nanodiscs, treated with a 10-fold molar excess of carbenoxolone

2026-01-21 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBj 2024-07-11 2026-01-21 2026-01-21 2026-01-21 National Research Foundation (NRF, Korea) RS-2023-00217798 Korea, Republic Of Other private SUHF-18010097 Korea, Republic Of Other private Korea, Republic Of Hemichannel sub-structure of Cx36/GJD2 gap junction intercellular channel (FN conformation) in soybean polar lipid nanodiscs, treated with a 10-fold molar excess of carbenoxolone Jang HS Gap junction, Connexin36, Inhibitor, Carbenoxolone, MEMBRANE PROTEIN Lee CW Jang HS Woo JS Structural insights into the closing mechanism of gap junction intercellular channels by carbenoxolone To Be Published 0353 EMD-60774 other EM volume 9ipo FULLOVERLAP EMDB EMD-60774 other EM volume EMDB EMD-60779 associated EM volume Connexin36 Connexin36 0 1

BRIL-fused connexin36 gap junction intercellular channel is reconstituted into lipid nanodiscs with soybean polar lipids and MSP1E1. Carbenoxolone dissolved in 10% (v/v) DMSO is treated to the sample with 10-fold molar excess to Cx36 protomer.

Homo sapiens Gap junction delta-2 protein,Soluble cytochrome b562 Homo sapiens 0.041035426 6 Homo sapiens LEVO MGEWTILERLLEAAVQQHSTMIGRILLTVVVIFRILIVAIVGETVYDDEQTMFVCNTLQPGCNQACYDRAFPISHIRYWV FQIIMVCTPSLCFITYSVHQSAKQRERRADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKS PDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLKLRRQEGISRFYIIQVVFRNALEIGF LVGQYFLYGFSVPGLYECNRYPCIKEVECYVSRPTEKTVFLVFMFAVSGICVVLNLAELNHLGWRKIKLAVRGAQAKRKS IYEIRNKDLPRVSVPNFGRTQSSDSAYVSRGDMLEVLFQ Q9UKL4 P0ABE7 Q9UKL4 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE 0.0006779329999999999 12 MC3 TETRADECANE 0.000198388 18 C14 singleParticle particle 3.0 7.5 20.0 C8H18N2O4S HEPES 150.0 KCl Potassium chloride 2.0 C2H6OS beta-mercaptoethanol

20mM HEPES(pH 7.5), 150mM KCl, 2mM beta-mercaptoethanol

Quantifoil R1.2/1.3 COPPER 200 CARBON HOLEY GLOW DISCHARGE 60 OTHER ETHANE 95 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 0.75 2.25 96000.0 NITROGEN FEI FALCON IV (4k x 4k) COUNTING 4096 4096 2028 6.79 50.0 1 860614 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION 2.41 FSC 0.143 CUT-OFF cryoSPARC 76373 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC emd_60779.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 250 250 250 0 0 0 250 250 250 202.5 202.5 202.5 90.0 90.0 90.0 X Y Z -0.20587254 0.48799005 0.0010675406 0.018537281 0.81 0.81 0.81 0.05 AUTHOR ::::EMDATABANK.org::::EMD-60779:::: emd_60779_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 250 250 250 0 0 0 250 250 250 202.5 202.5 202.5 90.0 90.0 90.0 X Y Z -0.3800397 0.57443815 0.0007426432 0.047091 0.81 0.81 0.81 AUTHOR ::::EMDATABANK.org::::EMD-60779:::: emd_60779_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 250 250 250 0 0 0 250 250 250 202.5 202.5 202.5 90.0 90.0 90.0 X Y Z -0.3142831 0.5366181 0.0007391994 0.047007505 0.81 0.81 0.81 AUTHOR ::::EMDATABANK.org::::EMD-60779::::