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    <admin>
        <current_status>
            <date>2026-03-04</date>
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            <processing_site>PDBj</processing_site>
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        <revision_history>
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        <sites>
            <deposition>PDBj</deposition>
            <last_processing>PDBj</last_processing>
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        <key_dates>
            <deposition>2024-07-25</deposition>
            <header_release>2026-01-28</header_release>
            <map_release>2026-01-28</map_release>
            <update>2026-03-04</update>
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        <grant_support>
            <grant_reference>
                <funding_body>Max Planck Society</funding_body>
                <country>Germany</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Jung foundation</funding_body>
                <country>Germany</country>
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        </grant_support>
        <title>In situ cryo-electron tomogram of 4days WT nucleus (trimer close to NE)</title>
        <authors_list>
            <author ORCID="0000-0002-5121-2164">Qu L</author>
            <author ORCID="0000-0002-7710-804X">Tang X</author>
            <author ORCID="0000-0001-8154-8809">Baumeister W</author>
        </authors_list>
        <keywords>proteasome, trimer, complex, PSG, paracrystalline, HYDROLASE</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Tang X</author>
                    <author order="2">Qu L</author>
                    <author order="3">Wilfling F</author>
                    <author order="4">Beck F</author>
                    <author order="5">Ernst OP</author>
                    <author order="6">Schulman BA</author>
                    <author order="7">Baumeister W</author>
                    <author order="8">Enenkel C</author>
                    <title>Metabolically regulated proteasome supramolecular organization in situ.</title>
                    <journal_abbreviation>Cell</journal_abbreviation>
                    <volume>189</volume>
                    <first_page>1153</first_page>
                    <last_page>1169.e16</last_page>
                    <year>2026</year>
                    <external_references type="PUBMED">41605212</external_references>
                    <external_references type="DOI">doi:10.1016/j.cell.2025.12.035</external_references>
                    <external_references type="ISSN">1097-4172</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <db_name>EMDB</db_name>
                <accession_id>EMD-60951</accession_id>
                <content_type>associated EM volume</content_type>
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        <name>26S proteasome trimer</name>
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                <name>26S proteasome trimer</name>
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                    <macromolecule>
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                    <macromolecule>
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                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>8</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>9</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>10</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>11</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>12</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>13</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>14</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>15</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>16</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>17</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>18</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>19</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>20</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>21</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>22</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>23</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>24</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>25</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>26</macromolecule_id>
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                    <macromolecule>
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                    <macromolecule>
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                    <macromolecule>
                        <macromolecule_id>29</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>30</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>31</macromolecule_id>
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                    <macromolecule>
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                    <macromolecule>
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                    <macromolecule>
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                <natural_source database="NCBI">
                    <organism ncbi="559292">Saccharomyces cerevisiae S288C</organism>
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            <aggregation_state>cell</aggregation_state>
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                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>6.8</ph>
                        <details>YPD buffer</details>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE-PROPANE</cryogen_name>
                    </vitrification>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage>30</voltage>
                            <current>0.1</current>
                            <duration>5</duration>
                            <temperature units="K">100</temperature>
                            <initial_thickness>500</initial_thickness>
                            <final_thickness>150</final_thickness>
                            <details>The value given for _em_focused_ion_beam.instrument is FEI Aquilos. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file.</details>
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            <microscopy_list>
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                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">3.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">4.0</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON IV (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">2.7</average_electron_dose_per_image>
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                    <number_images_used>37</number_images_used>
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                <ctf_correction>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
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            <space_group>1</space_group>
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        <spacing>
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            <y>1024</y>
            <z>320</z>
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        <cell>
            <a units="Å">9502.72</a>
            <b units="Å">9502.72</b>
            <c units="Å">2969.5999</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
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        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
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            <average>764.390260000000012</average>
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            <y units="Å">9.28</y>
            <z units="Å">9.28</z>
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                <source>AUTHOR</source>
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