2025-08-13 REL PDBj INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update PDBj PDBj 2024-09-13 2025-08-06 2025-08-06 2025-08-13 Japan Society for the Promotion of Science (JSPS) 20H02911 Japan Japan Society for the Promotion of Science (JSPS) 23K19283 Japan Class 3 state of the GfsA KSQ-ancestralAT chimeric didomain in complex with the GfsA ACP domain Chisuga T Liao Z Adachi N Kawasaki M Moriya T Senda T Kudo F Eguchi T Miyanaga A decarboxylase, polyketide synthase, LYASE Chisuga T Takinami S Liao Z Karasawa M Adachi N Kawasaki M Moriya T Senda T Terada T Kudo F Eguchi T Nakano S Ito S Miyanaga A Ancestral sequence reconstruction as a tool for structural analysis of modular polyketide synthases. Nat Commun UK 16 6847 6847 2025 40715098 doi:10.1038/s41467-025-62168-0 2041-1723 9jj9 FULLOVERLAP EMDB EMD-61520 associated EM volume
Class 3 state of the GfsA KSQ-ancestralAT chimeric didomain in complex with the GfsA ACP domain
 Class 3 state of the GfsA KSQ-ancestralAT chimeric didomain in complex with the GfsA ACP domain Class 3 state of the GfsA KSQ-ancestralAT chimeric didomain in complex with the GfsA ACP domain 0 1 2 Streptomyces graminofaciens Polyketide synthase GfsA Streptomyces graminofaciens 0.063183352
GfsA KSQ-ancestralAT
2 Escherichia coli BL21(DE3) LEVO HMGRSQNSEFETASDEPIAVIGLSCRLPKASGPQELWQLLDDGASAVTRVPADRETPPSTEEESADGEAAGARWGGFLDR VDTFDAGFFGISPREAAAMDPQQRLVLELSWEALEGAGLVPATLRDTGLGVFVGAARDDYATLYRRREGRAVDHHAMTGL HRSLIANRISYALGAHGPSMVVDTGCSSSLVAVHLACESLRRGESDIALAGGVNLNIAAESARETAAFGGLSPDGQCFTF DARANGFVRGEGGGLVVLKTLRRALADGDLVHGVILASAVNNDGPSDTLTTPSRRAQESLLTRVYRRAGVTPTEVGYVEL HGTGTKVGDPIEAAALGAVLGTGRDTPLPVGSIKTNIGHLEGAAGIAGLIKALLQLRRRRLVPSLNFSTPNPDIPLDALN LRVQQESAPWATPSGGGRTLVAGVSSFGMGGTNCHVVVSAAPVPEDGETTSEAGATGPDSGPALLPWVVSARSPQALRDQ AGRLAAWADSPAGREASPVDIGWSLATSRTHFEYRAVVSGSDRDELVASLRALASGSPVTAAGAVDGRAEPVALLSAVGE LFADGYPVDWTAYFAGWPAARVELPTYAFQRSRHWLENVPELAVS E0D202 E0D202 2.3.1.- Polyketide synthase Streptomyces graminofaciens 0.010244458 1 Escherichia coli BL21(DE3) LEVO HMPREPVTPDSDHPDPVETVRQLTAHVLGLTAAADVEMTRSFKDLGFDSLMSVELRDRLCAATGLSLATTLLYDHPSPAE TAEFVRARLTGDEA E0D202 N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide 0.00038333499999999995 1 9EF singleParticle particle 4.0 8.0 5.0 HEPES-NaOH 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 50.0 NaCl sodium chloride Quantifoil R1.2/1.3 COPPER 300 CARBON HOLEY GLOW DISCHARGE AIR ETHANE 100 291 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.8 2.0 FEI FALCON IV (4k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.71 FSC 0.143 CUT-OFF 105895 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_61520.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 296.96 296.96 296.96 90.0 90.0 90.0 X Y Z -0.63854414 0.91309834 -0.00028803296 0.015691537 0.58 0.58 0.58 0.07 AUTHOR emd_61520_msk_1.map emd_61520_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 296.96 296.96 296.96 90.0 90.0 90.0 X Y Z -0.037278414 1.9179621 0.00067288184 0.019654531 0.58 0.58 0.58 AUTHOR emd_61520_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 296.96 296.96 296.96 90.0 90.0 90.0 X Y Z -0.38499248 0.5197201 0.0000014427427 0.035698924 0.58 0.58 0.58 AUTHOR emd_61520_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 296.96 296.96 296.96 90.0 90.0 90.0 X Y Z -0.36302742 0.47141168 0.0000022908873 0.035878047 0.58 0.58 0.58 AUTHOR