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            <date>2025-10-29</date>
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            <update>2025-10-29</update>
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        <grant_support>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <country>China</country>
            </grant_reference>
        </grant_support>
        <title>acifran-HCAR3-Gi complex</title>
        <authors_list>
            <author>Binghao Z</author>
            <author>Fang Y</author>
        </authors_list>
        <keywords>GPCR complex, MEMBRANE PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Binghao Z</author>
                    <author order="2">Fang Y</author>
                    <title>acifran-HCAR3 complex</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <details>acifran-HCAR3-Gi complex</details>
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    </crossreferences>
    <sample>
        <name>Cryo-EM Structure of the acifran HCAR3-Gi complex</name>
        <supramolecule_list>
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                <name>Cryo-EM Structure of the acifran HCAR3-Gi complex</name>
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                <macromolecule_list>
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                    </macromolecule>
                </macromolecule_list>
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                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
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                <name>Hydroxycarboxylic acid receptor 3</name>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.044532300999999996</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="7108">Spodoptera frugiperda</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MNRHHLQDHFLEIDKKNCCVFRDDFIAKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLAVADFLLIICL
PFVMDYYVRRSDWKFGDIPCRLVLFMFAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKISNWTAAIISCLLWGITVGLTV
HLLKKKLLIQNGTANVCISFSICHTFRWHEAMFLLEFFLPLGIILFCSARIIWSLRQRQMDRHAKIKRAITFIMVVAIVF
VICFLPSVVVRIHIFWLLHTSGTQNCEVYRSVDLAFFITLSFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKITGE
PDNNRSTSVELTGDPNKTRGAPEALIANSGEPWSPSYLGPTSNNHSKKGHCHQEPASLEKQLGCCIE</string>
                    <external_references type="UNIPROTKB">P49019</external_references>
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                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
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                <molecular_weight>
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                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="7108">Spodoptera frugiperda</recombinant_organism>
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                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>TLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIA
IIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIA
QPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRM
HESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHF
TCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF</string>
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                </molecular_weight>
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                    <recombinant_organism ncbi="7108">Spodoptera frugiperda</recombinant_organism>
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SYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFAT
GSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDD
GMAVATGSWDSFLKIWN</string>
                    <external_references type="UNIPROTKB">P62873</external_references>
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                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
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                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
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                <enantiomer>LEVO</enantiomer>
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                    <external_references type="UNIPROTKB">P59768</external_references>
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                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
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                <molecular_weight>
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                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="7108">Spodoptera frugiperda</recombinant_organism>
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                <enantiomer>LEVO</enantiomer>
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LQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCR
SSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL</string>
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                <number_of_copies>1</number_of_copies>
                <formula>P9X</formula>
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