Cryo-EM structure of the heterotrimeric interleukin-2 receptor in complex with interleukin-2 and anti-CD25 Fab S417

2026-02-18 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.pdbx_database_id_DOI _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.pdbx_database_id_DOI _citation.year _em_admin.last_update DATABASE_REFERENCES citation em_admin _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update PDBj PDBj 2024-11-15 2025-11-19 2025-11-19 2026-02-18 Not funded Cryo-EM structure of the heterotrimeric interleukin-2 receptor in complex with interleukin-2 and anti-CD25 Fab S417 Katsura K Matsumoto T Shirouzu M Shirouzu M CYTOKINE, IMMUNE SYSTEM Itoh-Nakadai A Liang M Shindo M Bibi C Tomizawa-Murasawa M Fujiki S Kaneko A Kanamaru E Hashimoto M Kajita H Ando Y Kojima M Moody J Iwasaki M Takagi S Nakagawa R Agrawal S Amitani-Iijima H Sato K Sorimachi Y Suzuki N Fukami T Hanada K Morita S Katsura K Matsumoto T Kobayashi M Kato M Negishi Y Shirouzu M Najima Y Takubo K Hon CC Uchida N Taniguchi S Momozawa Y Carninci P Shultz LD Saito Y de Hoon M Shin JW Ishikawa F CXCR4 induces memory formation over exhaustion in CAR-T cells to achieve durable leukemia targeting. Nat Commun UK 17 101 101 2026 41587986 doi:10.1038/s41467-025-67745-x 2041-1723 9kmc FULLOVERLAP EMDB EMD-62427 associated EM volume

Cryo-EM structure of the heterotrimeric interleukin-2 receptor complex with interleukin-2 and CD25 Fab

Heterotrimeric interleukin-2 receptor in complex with interleukin-2 and Fab S417 Heterotrimeric interleukin-2 receptor in complex with interleukin-2 and Fab S417 0 1 2 3 4 5 6 Interleukin-2 receptor subunit alpha 1 1 Homo sapiens Interleukin-2 receptor subunit beta 1 2 Homo sapiens Cytokine receptor common subunit gamma 1 3 Homo sapiens Interleukin-2 1 4 Homo sapiens Fab S417 1 5 6 sythetic construct Interleukin-2 receptor subunit alpha Homo sapiens 0.026625502 1 Homo sapiens LEVO ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQ KERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQ LICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEYQGGGGSGGGGSGGGGSHHHHHH P01589 Interleukin-2 receptor subunit beta Homo sapiens 0.025824219 1 Trichoplusia ni LEVO AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWPDRRRWNQTCELLPVSQASWACNLILGAPDSQKLTTVDIVTL RVLCREGVRWRVMAIQDFKPFENLRLMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEARTLSPGHTWEEAPLLTL KQKQEWICLETLTPDTQYEFQVRVKPLQGEFTTWSPWSQPLAFRTKPAALGKDTHHHHHHHH P14784 Cytokine receptor common subunit gamma Homo sapiens 0.028628815999999998 1 Trichoplusia ni LEVO LNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWQSSSEPQPTNLTLHYWYKNSDNDKVQKC SHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHC LEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKENPHHHHHHH H P31785 Interleukin-2 Homo sapiens 0.015435978999999999 1 Escherichia coli LEVO APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHL RPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT P60568 Fab S417 heavy chain synthetic construct 0.024459379 1 Homo sapiens LEVO QVQLHQSGDDLVKPGASVKLSCRASGYTFTSYWINWVKQRPGQGLEWIGRILPGTNSIYYSEIFKGKASLTVDTSSNTAY IQLSSLSSEDSAVYFCARLLWNKGAMDYWGQGTSVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSW NSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKAEPKSCENLYFQ Fab S417 light chain synthetic construct 0.023004434 1 Homo sapiens LEVO QIVLSQSPVILSASPGEKVTMTCRASSGVSSIHWYQQQPGSSPKPWIYDTSSLASGVPARFSGSGSGTSYSLTISRVEAE DAATYYCQQWSSNPPTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQE SVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 1 NAG singleParticle particle 8.0 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.8 2.0 GATAN K3 (6k x 4k) 50.4 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.97 FSC 0.143 CUT-OFF 353002 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_62427.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 314.83 314.83 314.83 90.0 90.0 90.0 Z Y X -3.6250217 4.878181 0.000000000000098 0.05864429 0.8285 0.8285 0.8285 0.4 AUTHOR ::::EMDATABANK.org::::EMD-62427:::: emd_62427_msk_1.map emd_62427_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 314.83 314.83 314.83 90.0 90.0 90.0 X Y Z -0.10903938 0.21712384 0.000092692695 0.0070669935 0.8285 0.8285 0.8285 AUTHOR ::::EMDATABANK.org::::EMD-62427:::: emd_62427_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 314.83 314.83 314.83 90.0 90.0 90.0 X Y Z -0.104904965 0.2312648 0.00009500046 0.007059284 0.8285 0.8285 0.8285 AUTHOR ::::EMDATABANK.org::::EMD-62427::::