Bat MERSr-CoV NeoCoV Nsp1 bound to the Human 40S Ribosomal subunit-State1

2025-11-26 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBc 2024-11-18 2025-11-26 2025-11-26 2025-11-26 National Natural Science Foundation of China (NSFC) China Bat MERSr-CoV NeoCoV Nsp1 bound to the Human 40S Ribosomal subunit-State1 Yuan S Yan R Wu M 40S, Nsp1, betacoronaviruses, cryo-EM, RIBOSOME Yan R Wu M Yuan S Strategic Variations in Sarbecovirus and Merbecovirus Nsp1 Linker Regions for Ribosomal Rotation Defense To Be Published 0353 9kmx FULLOVERLAP EMDB EMD-62448 associated EM volume

The complex of Bat MERSr-CoV NeoCoV Nsp1 and Human 40S Ribosomal subunit The complex of Bat MERSr-CoV NeoCoV Nsp1 and Human 40S Ribosomal subunit 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Homo sapiens 18S ribosomal RNA Homo sapiens 0.602777875 1 UACCUGGUUGAUCCUGCCAGUAGCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUACGCACGGCCGGUACAG UGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUGGUUCCUUUGGUCGCUCGCUCCUCUCCUACUUGGAUAACUGUGGUAAU UCUAGAGCUAAUACAUGCCGACGGGCGCUGACCCCCUUCGCGGGGGGGAUGCGUGCAUUUAUCAGAUCAAAACCAACCCG GUCAGCCCCUCUCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCUUUGGUGACUCUAGAUAACCUCGGGCCGAUCGCACG CCCCCCGUGGCGGCGACGACCCAUUCGAACGUCUGCCCUAUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGUGACC ACGGGUGACGGGGAAUCAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCG CGCAAAUUACCCACUCCCGACCCGGGGAGGUAGUGACGAAAAAUAACAAUACAGGACUCUUUCGAGGCCCUGUAAUUGGA AUGAGUCCACUUUAAAUCCUUUAACGAGGAUCCAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCA AUAGCGUAUAUUAAAGUUGCUGCAGUUAAAAAGCUCGUAGUUGGAUCUUGGGAGCGGGCGGGCGGUCCGCCGCGAGGCGA GCCACCGCCCGUCCCCGCCCCUUGCCUCUCGGCGCCCCCUCGAUGCUCUUAGCUGAGUGUCCCGCGGGGCCCGAAGCGUU UACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCCCGAGCCGCCUGGAUACCGCAGCUAGGAAUAAUGGAAUAGGACCGCG GUUCUAUUUUGUUGGUUUUCGGAACUGAGGCCAUGAUUAAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGCUAGAGGU GAAAUUCUUGGACCGGCGCAAGACGGACCAGAGCGAAAGCAUUUGCCAAGAAUGUUUUCAUUAAUCAAGAACGAAAGUCG GAGGUUCGAAGACGAUCAGAUACCGUCGUAGUUCCGACCAUAAACGAUGCCGACCGGCGAUGCGGCGGCGUUAUUCCCAU GACCCGCCGGGCAGCUUCCGGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUAUGGUUGCAAAGCUGAAACUUAAAGGAA UUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGAAACCUCACCCGGCCCGGACACG GACAGGAUUGACAGAUUGAUAGCUCUUUCUCGAUUCCGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGCGAUUU GUCUGGUUAAUUCCGAUAACGAACGAGACUCUGGCAUGCUAACUAGUUACGCGACCCCCGAGCGGUCGGCGUCCCCCAAC UUCUUAGAGGGACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACAGGUCUGUGAUGCCCUUAGAUGUCCGGGGCUG CACGCGCGCUACACUGACUGGCUCAGCGUGUGCCUACCCUACGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUG AUGGGGAUCGGGGAUUGCAAUUAUUCCCCAUGAACGAGGAAUUCCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGAUUAAG UCCCUGCCCUUUGUACACACCGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGG UCGGCCCACGGCCCUGGCGGAGCGCUGAGAAGACGGUCGAACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAAGGUUU CCGUAGGUGAACCUGCGGAAGGAUCAUUA OZ112085.1 Small ribosomal subunit protein uS2 Homo sapiens 0.032883938 1 Homo sapiens LEVO MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSR NTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIA IPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTA TQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS P08865 Small ribosomal subunit protein eS1 Homo sapiens 0.030002061 1 Homo sapiens LEVO MAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVA FRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQ VRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGK ATGDETGAKVERADGYEPPVQESV P61247 Small ribosomal subunit protein uS5 Homo sapiens 0.031376516 1 Homo sapiens LEVO MADDAGAAGGPGGPGGPGMGNRGGFRGGFGSGIRGRGRGRGRGRGRGRGARGGKAEDKEWMPVTKLGRLVKDMKIKSLEE IYLFSLPIKESEIIDFFLGASLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKL SIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDCYTSARGCTATLGNFAKAT FDAISKTYSYLTPDLWKETVFTKSPYQEFTDHLVKTHTRVSVQRTQAPAVATT P15880 Small ribosomal subunit protein uS3 Homo sapiens 0.026729368999999996 1 Homo sapiens LEVO MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFP EGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHS GDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPV PTA P23396 4.2.99.18 Small ribosomal subunit protein eS4, X isoform Homo sapiens 0.029654868999999997 1 Homo sapiens LEVO MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDI TYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTI QIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPR GKGIRLTIAEERDKRLAAKQSSG P62701 Small ribosomal subunit protein uS7 Homo sapiens 0.022913453 1 Homo sapiens LEVO MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHG RNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGARE AAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR P46782 Small ribosomal subunit protein eS6 Homo sapiens 0.028779921999999996 1 Homo sapiens LEVO MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKG HSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRK PLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAREKRQEQIAKRRRLSSLRAS TSKSESSQK P62753 Small ribosomal subunit protein eS7 Homo sapiens 0.022168914 1 Homo sapiens LEVO MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLV RELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDK AQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL P62081 Small ribosomal subunit protein eS8 Homo sapiens 0.024263386999999997 1 Homo sapiens LEVO MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIID VVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISS LLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK P62241 Small ribosomal subunit protein uS4 Homo sapiens 0.022641563999999996 1 Homo sapiens LEVO MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRR LVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFS LRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED P46781 Small ribosomal subunit protein eS10 Homo sapiens 0.018933846 1 Homo sapiens LEVO MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLR DYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGR GQPPQ P46783 Small ribosomal subunit protein uS17 Homo sapiens 0.018468826 1 Homo sapiens LEVO MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKM KMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF P62280 Small ribosomal subunit protein eS12 Homo sapiens 0.014538986999999998 1 Homo sapiens LEVO MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVD DNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK P25398 Small ribosomal subunit protein uS15 Homo sapiens 0.017259389 1 Homo sapiens LEVO MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGL APDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA P62277 Small ribosomal subunit protein uS11 Homo sapiens 0.016302772 1 Human adenovirus sp. LEVO MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQD VAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL P62263 Small ribosomal subunit protein uS19 Homo sapiens 0.017076206999999996 1 Homo sapiens LEVO MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHL RDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK P62841 Small ribosomal subunit protein uS9 Homo sapiens 0.016477377 1 Homo sapiens LEVO MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQ IYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR P62249 Small ribosomal subunit protein eS17 Homo sapiens 0.015578156000000001 1 Homo sapiens LEVO MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERER RDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV P08708 Small ribosomal subunit protein uS13 Homo sapiens 0.017759776999999997 1 Homo sapiens LEVO MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIP DWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK P62269 Small ribosomal subunit protein eS19 Homo sapiens 0.016091562 1 Homo sapiens LEVO MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYG GRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH P39019 Small ribosomal subunit protein uS10 Homo sapiens 0.013398763000000001 1 Homo sapiens LEVO MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRF QMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA P60866 Small ribosomal subunit protein eS21 Homo sapiens 0.009124388999999998 1 Homo sapiens LEVO MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVS KNF P63220 Small ribosomal subunit protein uS8 Homo sapiens 0.014865554999999999 1 Homo sapiens LEVO MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFD VQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF P62244 Small ribosomal subunit protein uS12 Homo sapiens 0.015844665999999997 1 Homo sapiens LEVO MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK ITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS P62266 Small ribosomal subunit protein eS24 Homo sapiens 0.015107924 1 Homo sapiens LEVO MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLD YAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKK P62847 Small ribosomal subunit protein eS25 Homo sapiens 0.013776224 1 Homo sapiens LEVO MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIR GSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA P62851 Small ribosomal subunit protein eS26 Homo sapiens 0.011673847999999999 1 Homo sapiens LEVO TKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEASVFDAYVLPKLYVKLHYCVSCVIHS KVVRNRSREARKDRTPPPRFR P62854 Small ribosomal subunit protein eS27 Homo sapiens 0.009210843 1 Homo sapiens LEVO PLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRR KQ P42677 Small ribosomal subunit protein eS28 Homo sapiens 0.007007069 1 Homo sapiens LEVO QPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRL P62857 Small ribosomal subunit protein uS14 Homo sapiens 0.0065596249999999995 1 Homo sapiens LEVO GHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD P62273 Small ribosomal subunit protein eS30 Homo sapiens 0.006302479999999999 1 Homo sapiens LEVO GSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS P62861 Small ribosomal subunit protein eS31 Homo sapiens 0.008774459

KKRKKKSCTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYC

1 Homo sapiens LEVO KKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFN P62979 Small ribosomal subunit protein RACK1 Homo sapiens 0.034857355 1 Homo sapiens LEVO MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALS GSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNS SNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALC FSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIG P63244 Small ribosomal subunit protein eS32 Homo sapiens 0.003473451 1 Homo sapiens LEVO MRAKWRKKRMRRLKRKRRKMRQRSK P62945 ORF1ab polyprotein SARS bat coronavirus 0.021405541

The sequence of organism SARS bat coronavirus is not available during the biocuration, replaced by S5PWH2 temporarily.

1 Escherichia coli LEVO MSFVAGVTAQGARGMYRAALNSEKHQDHVSLTVPLCGAGDLVTKLSPWFMDGYEACEAVKVMLSNNEQLLFVPIHLVGYT KHLPGPRVYLVERLIKCVDPFMVNQLAYSSSANDGLVGTTLQGKPIGMFFPYDAELATGEETILLRKYGRGGYHYKRVPY QRNNTSFSEWIDDLEADPKGKFAQNLLKKLTGG S5PWH2 singleParticle particle 8.0 ETHANE JEOL CRYO ARM 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 GATAN K3 (6k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.89 FSC 0.143 CUT-OFF 53238 ANGULAR RECONSTITUTION ANGULAR RECONSTITUTION emd_62448.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 486.4 486.4 486.4 90.0 90.0 90.0 X Y Z -1.0934427 1.9154181 0.00043956324 0.055558752 0.95 0.95 0.95 0.192 AUTHOR ::::EMDATABANK.org::::EMD-62448:::: emd_62448_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 486.4 486.4 486.4 90.0 90.0 90.0 X Y Z -1.3241047 2.0388496 0.00085575256 0.17861955 0.95 0.95 0.95 AUTHOR ::::EMDATABANK.org::::EMD-62448:::: emd_62448_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 486.4 486.4 486.4 90.0 90.0 90.0 X Y Z -1.2948316 2.0924742 0.0008534292 0.17993048 0.95 0.95 0.95 AUTHOR ::::EMDATABANK.org::::EMD-62448::::