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            <date>2025-12-24</date>
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            <header_release>2025-12-24</header_release>
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            <update>2025-12-24</update>
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                <funding_body>Not funded</funding_body>
                <country>China</country>
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        </grant_support>
        <title>Cryo-EM structure of amyloid peptide-silk block protein fibril, Type 2</title>
        <authors_list>
            <author>Zhang YL</author>
            <author>Dai B</author>
        </authors_list>
        <keywords>PROTEIN FIBRIL, amyloid, silk</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Zhang YL</author>
                    <author order="2">Dai B</author>
                    <title>Cryo-EM structure of amyloid peptide-silk block protein fibril, Type 2</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
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                <db_name>EMDB</db_name>
                <accession_id>EMD-62779</accession_id>
                <content_type>associated EM volume</content_type>
                <details>Cryo-EM structure of amyloid peptide-silk block protein fibril, Type 2</details>
            </db_reference>
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    </crossreferences>
    <sample>
        <name>Cryo-EM structure of amyloid peptide-silk block protein fibril, Type 2</name>
        <supramolecule_list>
            <organelle_or_cellular_component_supramolecule supramolecule_id="1">
                <name>Cryo-EM structure of amyloid peptide-silk block protein fibril, Type 2</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="256737">Latrodectus hesperus</organism>
                </natural_source>
            </organelle_or_cellular_component_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>silk-G6V</name>
                <natural_source database="NCBI">
                    <organism ncbi="256737">Latrodectus hesperus</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.048405319999999995</theoretical>
                </molecular_weight>
                <details>The protein that forms the fibril is a block protein composed of 16 repetitive motifs. Due to the inability to precisely identify which specific repetitive motif contributes to the core sequence of the fibril, it is not possible to directly correlate the amino acid sequence of each chain with a particular repetitive motif of the protein. Based on the arrangement of these repetitive motifs, we ultimately constructed the atomic model.</details>
                <number_of_copies>9</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAKTKGTASGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRG
GLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVI
EVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGG
LGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGL
GGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEV
GGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLG
SQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSGRGGLGGQGAGGDVIEVGGAGQGGYGGLGSQGTSSGHHHHH
HHHHH</string>
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                        <ph>7.5</ph>
                    </buffer>
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                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </helical_preparation>
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                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
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