2025-11-12 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBj 2024-12-30 2025-11-12 2025-11-12 2025-11-12 Not funded Bacillus subtilis endospore crust protein CgeA Park M Kim D Baek Y Hyun J Ha NC Glycoprotein, Sporulation, Bacillus subtilis, STRUCTURAL PROTEIN Park M Kim D Baek Y Jo E Hyun J Ha NC Cryo-EM structure of the glycosylated protein CgeA in the crust of Bacillus subtilis endospores. J.Microbiol KR 63 e2504013 e2504013 2025 41164959 doi:10.71150/jm.2504013 1976-3794 9l9d FULLOVERLAP EMDB EMD-62902 associated EM volume
Bacillus subtilis endospore crust protein CgeA
 Hexameric complex of Bacillus subtilis endospore crust protein CgeA Hexameric complex of Bacillus subtilis endospore crust protein CgeA 0 1 Bacillus subtilis subsp. subtilis str. 168 168 0.084 Spore crust protein CgeA Bacillus subtilis subsp. subtilis str. 168 168 0.014503417 6 Escherichia coli BL21(DE3) LEVO GAMGMSSENAQLKKDLIKAVLSPLFPTATEGGENMDSNLKALLDACIDQKVDESETVTAESILDPSLPARWIFARITPGT TISIVTDSGDMIGPVVFVAFCQVHGIVFVTQESSVTPAGQATTLIDVDKVESVTFFS P42089 singleParticle particle 0.2 8.0 20.0 Tris-HCl Tris buffer 150.0 NaCl Sodium chloride
20 mM Tris-HCl, 150 mM NaCl
 Quantifoil R1.2/1.3 COPPER 300 ETHANE 100 277.15 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.8 1.7 105000.0 NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) 2 10593 63.6 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 3.05 FSC 0.143 CUT-OFF cryoSPARC 4.5.1 88923 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_62902.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 158.976 158.976 158.976 90.0 90.0 90.0 X Y Z -0.20553471 0.32617107 -0.00005356577 0.0126782395 0.828 0.828 0.828 0.1 AUTHOR AlphaFold in silico model FLEXIBLE FIT
Initial local fitting was done using Coot and Chimera and then PHENIX was used for flexible fitting
REAL emd_62902_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 158.976 158.976 158.976 90.0 90.0 90.0 X Y Z -0.2639357 0.36785626 0.00021138939 0.020952383 0.828 0.828 0.828 AUTHOR emd_62902_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 158.976 158.976 158.976 90.0 90.0 90.0 X Y Z -0.23731567 0.3483206 0.00021810974 0.020963887 0.828 0.828 0.828 AUTHOR