2026-03-04 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBc 2025-01-17 2026-03-04 2026-03-04 2026-03-04 National Natural Science Foundation of China (NSFC) China Cryo-EM structure of D1R-Gs in complex with de novo designed GEM targeting TM3/4/5 Guo J Zhou Y Cheng S Zhang Y GPCR, GEM, GPCR exoframe modulator, D1R, MEMBRANE PROTEIN Cheng S Guo J Zhou Y Zhang Y GPCR exoframe modulator To Be Published 0353 9llf FULLOVERLAP EMDB EMD-63200 associated EM volume
Cryo-EM structure of the de novo designed bias-GEM in complex with D1R
 mGlu2-mGlu4 heterodimer bound Gi mGlu2-mGlu4 heterodimer bound Gi 0 2 Homo sapiens Expression tag,D(1A) dopamine receptor,LgBiT synthetic construct 0.08199074199999999 1 Spodoptera frugiperda LEVO DYKDDDDKEFADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVG QIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLLEVLFQGPRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILST LLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVI SVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAIS SSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAALERAAVHAKNCQTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVI MGVFVCCWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNA IETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQP ITQNGQHPTGSGGSGGGGSGGSGGSVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKI DIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNG NKIIDERLITPDGSMLFRVTINSGGS P21728 Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Homo sapiens 0.044284074 1 Spodoptera frugiperda LEVO MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQMRILHVNGFNGDSEKATKVQD IKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDC AQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYN MVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRV TRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL P63092 3.6.5.- Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Homo sapiens 0.041729582 1 Spodoptera frugiperda LEVO MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMH WGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGH TGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKA DRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWRLFKKIS P62873 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Homo sapiens 0.007861143 1 Spodoptera frugiperda LEVO MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL P59768 Nanoboy 35 Lama glama 0.015140741999999999 1 Escherichia coli LEVO QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLY LQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA De novo designed GPCR exoframe modulator targeting TM3/4/5 Homo sapiens 0.008862196 1 Spodoptera frugiperda LEVO GPPLPLSKIVILLIILLILSIIFLLLLYLLIKYFKSFNEPIPPMLKVFLIYCVLSLIWVIIYTIIEVLELLLRPPPG L-DOPAMINE 0.000153178 1 LDP singleParticle particle 7.5 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.6 3.0 FEI FALCON IV (4k x 4k) 52.0 1 NONE 2.64 FSC 0.143 CUT-OFF 228382 RANDOM ASSIGNMENT MAXIMUM LIKELIHOOD emd_63200.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 208.32 208.32 208.32 90.0 90.0 90.0 X Y Z -2.8159068 4.347193 -0.004693822 0.119109616 0.93 0.93 0.93 0.315 AUTHOR emd_63200_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 208.32 208.32 208.32 90.0 90.0 90.0 X Y Z -0.81204957 1.5069588 0.0005833043 0.060057797 0.93 0.93 0.93 AUTHOR emd_63200_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 208.32 208.32 208.32 90.0 90.0 90.0 X Y Z -0.7512435 1.564198 0.0005719444 0.06000907 0.93 0.93 0.93 AUTHOR