The overall cryo-EM map of the mouse CatSper trimer

2026-01-28 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update PDBj PDBc 2025-04-03 2025-12-31 2025-12-31 2026-01-28 National Natural Science Foundation of China (NSFC) China The overall cryo-EM map of the mouse CatSper trimer Xu Q Lin S Zhao Q Yan Z Wu J sperm, hyperactivation, calcium channel, PROTEIN TRANSPORT Xu Q Lin S Zhao Q Ru Y Kang H Zeng X Jiang M Yan Z Wu J Molecular basis of the higher-order assembly of CatSper. Proc.Natl.Acad.Sci.USA US 123 e2510754123 e2510754123 2026 41490491 doi:10.1073/pnas.2510754123 1091-6490 0040 PNASA6 9ubx FULLOVERLAP EMDB EMD-64028 associated EM volume

Cryo-EM structure of the mouse CatSper trimer

Homotrimer of the mouse CatSper Homotrimer of the mouse CatSper 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Mus musculus Cation channel sperm-associated protein 1 Mus musculus 0.029080652 3 Mus musculus LEVO LLGLQQMILSLTQSLGFETFIFIVVCLNTVILVAQTFTELEIRGEWYFMVLDSIFLSIYVLEAVLKLIALGLEYFYDPWN NLDFFIMVMAVLDFVLLQINSLSYSFYNHSLFRILKVFKSMRALRAIRVLRRLSILTSLHEVAGTLSGSLPSITAILTLM FTCLFLFSVVLRALFQDSDPKRFQNIFTTLFTLFTMLTLDDWSLIYIDNRAQGAWYIIPILMIYIVIQYFIFLNLVIAVL VDNFQMALLKG Q91ZR5 Cation channel sperm-associated protein 2 Mus musculus 0.033115477 3 Mus musculus LEVO PLSLWAGWVLDSSVFSKFIISLIFLNTFVLMVEIELMESTNTALWPVKLALEVADWFILLSFIVEILLMWLASFSLFWKD AWNVFDFFVTLLSLLPELVVLLGVPAHSVWLQLLRVCRVLRSLKLFARFRQIKVILLALVRALKSMTFLLMLLLIFFYIF AVTGVYFFREYSRSTIEGLEYNMFFSDLLNSLVTVFILFTLDHWYAVLQDIWKVPESSRVFSSIYVILWLLLGSIIFRNI IVAMMVTNFQNIRSELSEEMSHLEVQYKADMFKQQIIQRRQ A2ARP9 Cation channel sperm-associated protein 3 Mus musculus 0.032308949 3 Mus musculus LEVO ECQAYFRKVIKSTFFQIVMITTVTTNSFLLVLGTNYDIQFEFFRTFEVSELFFVSVYVCEFLMKVYVDPITYWKDGYNIL DVIILIILTIPYLLRKIKGNHSAYLHFADGIQSLRILKLISYSRGIRTLIIAVGETVYTVASVLTLLFLLMFVFAILGFC LFGVTDRGDLENWGNLASAFFTLFSLATVDGWTDLQEELDKRKFTVSRAFTILFILLASFIFLNMFVGVMIMHTEDSMKK FERDLTLERNLAIMEEKQIILKRQQEEVNRLMNTQKSG Q80W99 Cation channel sperm-associated protein 4 Mus musculus 0.02888043 3 Mus musculus LEVO DVMEKKDAWDVQEFITQMYIKQLLRHPAFQLLLAFLLLSNAITIALRTNSYLGQKHYELFSTIDDIVLTILICEVLLGWL NGFWIFWKDGWNILNFAIVFILFMGFFIKQLDMVAITYPLRVLRLVHVCMAVEPLARIIKVILQSMPDLANVMALILFFM LVFSVFGVTLFGAFVPKHFQNMGVALYTLFICITQDGWLDIYTDFQMDEREYAMEVGGAIYFAVFITLGAFIGLNLFVVV VTTNLEQMMK Q8BVN3 Cation channel sperm-associated auxiliary subunit beta Mus musculus 0.12158059399999999 3 Mus musculus LEVO VIHNKGKERTYFSCSGEGILTGLHTIKLFLTMDNLKVRCFFRNENQSPSKEILGLFTSGGLAPNMIITNSTFYGGYYFKL TPFSNRLEWLIDIPRQNITVNTDIAAVEQWMIKITMHEGLNIYDTEGTLLDLVREPILQWNLGRVLTEMEVRDLYPEVND IKVTKSPCANDVALIGFMMKPSSNGVFIGKTISGFWTYKECIWHDLTEIIYAELKDEHQGLTVIDLVLTNHFLVILTSLG LYVSSDLRYPTTSQIKLSRAEFCGFERVDYIRGNLWYNEKCFANRESFEVDYVTITFNRNRTLSESSSCFFSKEPFLHWL PCVFSTIKNEKSIPRVITFLIDQETDSGIYLFNVQDTKETYVTVAMLKDGKPSPRPKFPSFHFPSTFTLPLGMIFHPRSH FLYVYGSQIWVSMDGGNTFEMLCNLFSHHVTKTSNSFYTSDIVFIVEDGRILTTKAGLTTYSELGILKDAIFTLYYDQLG YIHKLTPENFDAGSKLLGHGNSGSIFGKRPDLGFEAILVPQYISTNEMYFFAHVPLTMPTNIQWKKRFKTIHLGKTIEFS KTGLANIKNVYMHKTEPVGFQTSIHTEIIVPFGIENSKDSPCLLSDLEITYSGKLYYTIKLLSKNPLHELKSTDVEKSVL IPGYSSFLIMNITDKWTASALATMPQAIKSNLKFLTGSWFLYNFGTAGGRKWSISTRQCNYWIQQDSLDFMSLNLVKYID VGNTIDFQFKIIPKAMSTFPIPPVSMVVGNPGLVEVKTQGVFDLNENYYLDIHVSGRFFQKGSTSIALVLWEGSSKCYAI TLLPTIKSSCSYLRTMHHTPGRHIPPEDWISGVHKDSQGFNMIKTLPINYRPPSHMGISIPLTDNFYHADPSKPIPRNQF HKSKETGKYKQCANVTSRAMCNCSEHQKFSHAVAFSDCKEKVHRFKFPVTQYPVVLEIFNERDKISAEPPYLVTMTEVNM RKNWQLKHNEPENVKKMKHYLEPLLKTPVYNPLGLNLTIQGSELFHFKVSVVPGVSFCELSEEFQIYVDEVPLPFPGHAL IAVATSVVLGVLIFIAFVFQLRNIH A2RTF1 Cation channel sperm-associated auxiliary subunit gamma 2 Mus musculus 0.119864367 3 Mus musculus LEVO HCTWLLVLNKFEKVGLHLSKDRFQDHEPIDTVAKVFQKLTDSPIDPSENYLSFPYYLQINFSCPGQNIEELARKGHLMGM KPMVQINYMYSVNFYRWEMENVQILMEAAPMRSTGYCPAEAMCVLNWYTPMPFKNGSVVSSVDIYTNGIGPFVSKKRFYV NMNGFLKRDASGKSLFAIGYESLVLKSSHFRLSKSRPLWYTVNHAPVFILGGFYDEKSILFSDSNFQDYVLLELSIDSCW VGSFYCPILGFSATIHDAIATESTLFIRQNQLVYYFTGTYITLFDKSHGSSRWVRVLPSECIKRLCPVYASGNGSEYVLA LTTGKNEGYIHIGTITDGLVSFEMVPDGWSVCEKLPGKNCSIDWATYIADERNLLLLVKIDSGQFYLVNFNTEFKTLNIL YKIPEFIPEAKELDFLVLLDTVTYTNTPMTPKGLFFNTLNNMLYIWGNFILQSYNREEFIFLADFPKESTIKYMVNSFKG QMAVVTENEEIWYFLEGGYDVYQVVPSQGWETYHNLQKMQKSSFHSEDESLVSLFFEDGKLFQLVYLFDVGKERLVKRLL PVGTLMEYNLPKPFTVVNQGNYQAISFTHTCPFKEIHLIDVPKKHHASRTESYVALPPLVSESLGFHNNNTLAVYQGLVY YLLWLHSKYDKPYADPVHDPTWRWWQHKTKDKDYFFYLFSNRLAAEGIYINMNAYQKLYNMSGDYGIPDLFFLDKGNWFT ITVVLLSHQDTFTSSDSQGPTINVDKKLAIAVTIADPECLSVTVTQDVLLNRNAVINKIKVIDKKRCSEQGMIGRNIKKT SMMLKVLGAPGNCIQRTYLGGIIQGFKVVPIFIGCPPGKRLAFDVSYTIMHSEEINKHYFDCVIKDAEMPCFLFRDLFQP FFLVQDLVTGDSGSFLGSYVLKVVGGGRTLNTIRDYTEEEIFRYNSPLDTTNSLIWKTKVERTTEDKKFYIMSHESPGVE WLCLENSPCYDIIPQSIYPPEFFFKLLVSNRGVDNSTYCDYKLTFIVHIHGLPLSSKRTSFIVMVSTSFFIALVVFYILF CL C6KI89 Cation channel sperm-associated auxiliary subunit delta Mus musculus 0.082834281 3 Mus musculus LEVO HTLCRVHTVRTGKVFKSNIQLQGDPLFYAFPNTFVLKNVCKADISVYLGQKVFLTIDNFESSLLPLTVPKSLAVGVPSIT SAHFVSGSLVLFVISGKGYSYDYYENTWRKLEGISEPVSHISGDVCCFKGSFCLELSNNLFAYLRGGQIPGTNIYFSDNG GFSFQLMNTDKLSHLTGTLGGIFHLHSMSQVGVLMVENNLGTFHYMEYPLNHSMGIAFSYKNLLEVIMKPYQRGFMVLWN QKSILVSSNSGQIVEHVRLIDQKIFTDLDVEHANINIYSVASNAYELAFLVAEDHLYYGSQSYMGTYVIKLPHQPLWSTH TSIYFEDIGILQVLTPVADPHFAAYDFDKCTVNVQSSLMDEKLALQPCNVELLESTMINTMFTIDMNSKLKLSALMIPRK GENPTPLVMVSNPHALGFKANLNEFGNTFDGNSKYKLDIELKQQHHWGNSDFNFTASIKRHAISSVTVDIADKTLSCVDL KPLSTLISVGCDMTKKIVVQNKISACTMGILNPVQLQKNYTYTIEKEAYDPINHNGEAQDDLIVFYEYKDLGCPRLVYYD KPWKPVVELWKNGIVEEIMNAEYVISEINGLVTYSYSLTAATANCRSQPQNWSTFESDIENEEPFLWNRENYVSCHEDNK DNPLLWPNVEYQVLGGQTNNKIIFGQRNGIYTFHLSVVDPYYSYCNLNTIFSVYVHGALPVTKFQPLLTILLMVTTTLLT AWLAYAIPKQLRS E9Q9F6 Cation channel sperm-associated auxiliary subunit epsilon Mus musculus 0.107004016 3 Mus musculus LEVO LWRYYINSQDYSIFSTRSSIKLEYEGNSFVSWKIPESCKVENTTSPKTTLHCKRAGIHTIKPIAGNQEVERHLTVDNSYI CYLWYFTVVDVYYNLSQIVTIWVYDPESASTEELIWTAKKPSLSSRVLTKQMNTLGQRPFIFTVEKRLTYHPGPLTSEGT WVIHLPMSSDDIAKVIRGNKVAFQDCFIANLYFMLTYPMTIISEPPGYEPLTVPPGSPLMLSWDTCISTFALLATDQETF QTNDSFQTWTRVRAPPGILSDAQRHSLRDVIIFDQGTLFLVDGTVYLRTEDEFTKLDESRGISETGILGFSKRRWCQIRY LYKLASKKSILIAWSKTTVYAGYATFRFVTLTDTAKLKDFLKLPQTDTLEVMSVEYLWHPLEAAVLLSHCSVCTTNTRNI RIVIYSAIFQTWTLQDFELQLPKEAILEFRFLYSAMPDIIMWDQHHVYYSYKNFTVVGTISTPSGETNLSSLSQGSKIHQ VLTDRIGNVVVKMENNVMFYIKADITEAVILHTWVNTTAKTVVLFDKSFEVCILYYNENLDEKYQLQTQPYPLILELQSI NKDLGDWCPYLAFQHNIHSQFYHMDKGESLTIWSQIVYPENRGLYIVVEHYGSSVMTWTQNLEYEIASGFCTKTMITRFF QTTNYELVDNYYQLQKENTGLMLLQFRPSEFSRTCLTAKPVFEIDVGCDSSKYIMVRGFNKSRCQRRDFSYVIDKELLRE SLSDNLKVRYDVAKYGCPLTLELGQMFQPIVELYDENGFIKIVDANFILWEIHGRNDYTFNSTMEQNGCINEAQTWDSMI EENPDIPLDDVWGPQNYRPCFSYAIGKPGDLGQPYEILNYSNKNHIKWPMTYAGMYVYRLKILDPNYSFCNLTTIFAIES LGMIPRSSVYLVAALIFVLMLTFISILVLSYFWYLKIYRQF P0DP43 EF-hand calcium-binding domain-containing protein 9 Mus musculus 0.020942268 3 Mus musculus LEVO MKLTPGCFLWYLYMDKIYCLLSLRNVKALMVYFHLLDVHHRNTLNDVLFFHFLQHVTNLNKSQIGMIFDLLDWTAVGEIG FDQFYVLICILLAHQDHLEDHFMYRHSRPVFELLDLDGEMNIGAANFQNYRFLFNIKKQELRDLFHDFDITGDRLLNYKE FKLYTIFCTDKSID Q9DAM2 Cation channel sperm-associated auxiliary subunit TMEM249 Mus musculus 0.019898578 3 Mus musculus LEVO MCWVMPPSVFVLEYYLDTLWKGTMLFIICLVFISCNVLREVKYQETWCFPAYGMVIGLWLMLSSIPQRRLVLNHTRGMYH FSIQGRTVCQGPMHLVYVRLALSSDAYGGRFFQLVLCGHKLEPLVLVQLSERYEQMEFLGRHLARKLNINYFDYLASSYR HVVRHWPL A0A2R8VHF7 Cation channel sperm-associated auxiliary subunit zeta Mus musculus 0.017504592 3 Mus musculus LEVO TANVSVSDVCEDFDEEGKSVRNRIRKYSQTISIRDSLNLEPEEIQQQARRELELCHGRSLEHGEDHEESETSLASSTSES LIFSLWKPHRTYWTEQQNRLPLPLMELMETEVLDILKKALITYRSTIGRNHFMTKELQGYIEGIRKRRN Q9CQP8 Solute carrier organic anion transporter family member 6C1 Mus musculus 0.06834968 3 Mus musculus LEVO PIVLPFVQRFNNIDGFMTLYVAAVLIHGALFAVVDMTLNIYQVQFSLTRTEWYLMDFSDYIASFVVAIIIAHFGSKGNRT RWIAASCILMGLESMLFAFPFFTYEIIIPGRQSIELCMEENEKRNIICGNSVPNRSKCIYFHIGGQCIHGIAGMPIYILG ITFIFDHIPTSSCGFYLAIGHSAYLIGYLLGMVGGLQNFQPPPKEKTVEIEPAKVYQLLQSGWWKTFLIIAAISFCVSFM MVCFPTSLPGAHKLRLAKRKEPPTIDRRLKDMKIQPHLKGFLHNIWHILKNPLMLTQAICKVSEYLTFNTSLYFLPHHLQ TQFLITPGIASLLTGAFVLPGGIIGHFLGGLIVDRLEMTNKNKLKFTLVTTVVSVGLFLLIFFVECQTTTFAGINEDYDG YGQLGNLTADCNEYCDCTTSLYTSICGRDEKEYFSPCFAGCKATKVSQTEKTYYNCSCIKEGLAASDDEGQFIDAIAGTC DSDCLKLPLFFAFYFSATVFSNMCSIPVISIILQSVPANFTSLSLGVTYAIVKFVASVPAPLLFRLSSAIACIYWDNNRC GGKERCWIYNKNILVYEFMGIWMSSQLIIVLLNIYAIQIHDVVVHGEITE Q8C0X7 Cation channel sperm-associated auxiliary subunit TMEM262 Mus musculus 0.013350882 3 Mus musculus LEVO MRWRDRIAVLCFPPGLMLTVAALILFFIHMGVFASDVHNFCVIHNYDHMSFRYTVVLIFSQVISIGWAAMGSLYAEMTGD KFLRCFALTILILNGAMFFNRLCLEFLAINYREER D3Z338 Unknown protein Mus musculus 0.002400951 3 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 27 NAG CHOLESTEROL 0.000386654 3 CLR singleParticle particle 7.4 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.5 2.5 GATAN K3 (6k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 6.8 FSC 0.143 CUT-OFF cryoSPARC 69708 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_64028.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 600 600 600 0 0 0 600 600 600 652.2 652.2 652.2 90.0 90.0 90.0 X Y Z -0.37576637 0.9998739 0.001660244 0.046525996 1.087 1.087 1.087 0.245 AUTHOR ::::EMDATABANK.org::::EMD-64028:::: emd_64028_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 600 600 600 0 0 0 600 600 600 652.2 652.2 652.2 90.0 90.0 90.0 X Y Z -1.7057048 1.8132972 0.001460817 0.21245903 1.087 1.087 1.087 AUTHOR ::::EMDATABANK.org::::EMD-64028:::: emd_64028_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 600 600 600 0 0 0 600 600 600 652.2 652.2 652.2 90.0 90.0 90.0 X Y Z -1.7766732 1.8306252 0.0014443541 0.21416001 1.087 1.087 1.087 AUTHOR ::::EMDATABANK.org::::EMD-64028::::