Cryo-EM structure of the HBsAg dimer and Complex with Fab

2026-02-18 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBc 2025-04-13 2026-02-18 2026-02-18 2026-02-18 Not funded Cryo-EM structure of the HBsAg dimer and Complex with Fab Liu Y Liao M Liu Z Ju B Zhang Z HBV antigen, dimer, Fab, complex, VIRAL PROTEIN/IMMUNE SYSTEM, VIRAL PROTEIN-IMMUNE SYSTEM complex Ju B Liu Z Yan H Liu Y Zhang L Ge X Wang X Si Z Zhou B Fan Q Wang M Li Y Lai W Gan J Wang H Zhao J Xia Y Liao M Zhang Z Characterisation of plasmablast-derived HBsAg-specific antibody and its structural basis for binding to native HBsAg dimer. Gut 2026 41663152 doi:10.1136/gutjnl-2025-336641 1468-3288 9ugo FULLOVERLAP EMDB EMD-64142 associated EM volume

complex of HBsAg dimer with Fab complex of HBsAg dimer with Fab 0 1 2 3 Homo sapiens Fab light chain Homo sapiens 0.023425979 2 Homo sapiens LEVO EIVMTQSPATLSVSPGERATLSCRASQSVSNNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQS EDSAVYYCQQHHSWPPFTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNS QESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC Fab heavy chain Homo sapiens 0.02412116 2 Homo sapiens LEVO QVQLVQSGAEVKKPGSSVKVYCKASGGTFRSYGLNWVRQAPGQGLEWMGRIVPIFGISNFAQKFQGRFTITADRSANTPY MELSSLRSEDTAVYYCVRGGAVAGAGSAGEGIFDYWDQGTLVIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC Middle S protein hepatitis B virus genotype A 0.011008200000000001

The sequence of organism hepatitis B virus genotype A is not available during the biocuration, replaced by B8QJB4 temporarily.

2 Yeast centromeric expression vector p416CYC LEVO RWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPI PSSWAFGKFLWEWASARF B8QJB4 singleParticle particle 7.5 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.0 GATAN K3 (6k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 4.0 FSC 0.143 CUT-OFF 53213 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_64142.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 218.88 218.88 218.88 90.0 90.0 90.0 X Y Z -0.055961 0.09352878 0.00008498955 0.0025482667 0.855 0.855 0.855 0.008 AUTHOR ::::EMDATABANK.org::::EMD-64142:::: emd_64142_msk_1.map emd_64142_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 218.88 218.88 218.88 90.0 90.0 90.0 X Y Z -0.04982162 0.084907584 0.000094603245 0.0017745048 0.855 0.855 0.855 AUTHOR ::::EMDATABANK.org::::EMD-64142:::: emd_64142_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 218.88 218.88 218.88 90.0 90.0 90.0 X Y Z -0.013342493 0.033686325 0.000085219355 0.0016919434 0.855 0.855 0.855 AUTHOR ::::EMDATABANK.org::::EMD-64142:::: emd_64142_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 218.88 218.88 218.88 90.0 90.0 90.0 X Y Z -0.011909735 0.030230349 0.00008736443 0.001695813 0.855 0.855 0.855 AUTHOR ::::EMDATABANK.org::::EMD-64142::::