Cryo-EM structure of VTC complex(Vtc5/Vtc4/Vtc3/Vtc1)

2026-01-28 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update PDBj PDBc 2025-04-21 2026-01-21 2026-01-21 2026-01-28 National Natural Science Foundation of China (NSFC) 32422041 China Cryo-EM structure of VTC complex(Vtc5/Vtc4/Vtc3/Vtc1) Zhang J Du Z Liu Z Transporter, Complex, polyP synthesis, Vacuolar membrane, TRANSPORT PROTEIN Zhang J Du Z Cheng M Zheng Z Jiang L Chen Y Yin P Liu Z Mechanistic insights into the regulation of polyphosphate polymerase VTC by the accessory subunit Vtc5. Sci China Life Sci CN 2026 41535663 doi:10.1007/s11427-025-3127-8 1869-1889 9umg FULLOVERLAP EMDB EMD-64273 associated EM volume

Vacuolar transporter chaperone (VTC) complex composed of Vtc1, Vtc3, Vtc4 and Vtc5 Vacuolar transporter chaperone (VTC) complex composed of Vtc1, Vtc3, Vtc4 and Vtc5 0 1 2 3 4 5 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Vacuolar transporter chaperone complex subunit 1 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.01325381 1 Homo sapiens LEVO PLLQRTPGKKIALPTRVEPKVFFANERTFLSWLNFTVMLGGLGVGLLNFGDKIGRVSAGLFTFVAMGTMIYALVTYHWRA AAIRRRGSGPYDDRLGPTLLCFFLLVAVIINFILRLKY P40046 Vacuolar transporter chaperone complex subunit 1 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.011592723 2 Homo sapiens LEVO VEPKVFFANERTFLSWLNFTVMLGGLGVGLLNFGDKIGRVSAGLFTFVAMGTMIYALVTYHWRAAAIRRRGSGPYDDRLG PTLLCFFLLVAVIINFILRLKYN P40046 Vacuolar transporter chaperone complex subunit 4 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.06028361299999999 1 Homo sapiens LEVO RQTTKYWVHPDNITELKLIILKHLPVLVFNTNKEFEREDSAITSIYFDNENLDLYYGRLRKDEGAEAHALAWYGGMSTDT IFVERKTHREDWTGEKSVKARFALKERHVNDFLKGKYTVDQVFAKMRKEGKKPMNEIENLEALASEIQYVMLKKKLRPVV RSFYNRTAFQLPGDARVRISLDTELTMVREDNFDGVDRTHKNWRRTDIGVDWPFKQLDDKDICRFPYAVLNVKLQTQLGQ EPPEWVRELVGSHLVEPVPKFSKFIHGVATLLNDKVDSIPFWLPQMDVDIRKPPLPTNIEITRPGRSDNEDNDFDEDDED DAALVAAMTNAPGNSLDIEESVGYGATSAPTSNTNHVVESANAAYYQRKIRNAENPISKKYYEIVAFFDHYFNGDQISKI PKGTTFDTQIRAPPGKTICVPVRVEPKVYFATERTYLSWLSISILLGGVSTTLLTYGSPTAMIGSIGFFITSLAVLIRTV MVYAKRVVNIRLKRAVDYEDKIGPGMVSVFLILSILFSFFCNLVAKL P47075 2.7.4.1 Vacuolar transporter chaperone 3 complex subunit 3 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.069612711 1 Homo sapiens LEVO ASFKSYKFWVHDDNIMEVKARILRHLPALVYASVPNENDDFVDNLESDVRVQPEARLNIGSKSNSLSSDGNSNQDVEIGK SKSVIFPQSYDPTITTLYFDNDFFDLYNNRLLKISGAPTLRLRWIGKLLDKPDIFLEKRTFTENTETGNSSFEEIRLQMK AKFINNFIFKNDPSYKNYLINQLRERGTQKEELEKLSRDFDNIQNFIVEEKLQPVLRATYNRTAFQIPGDQSIRVTIDSN IMYIREDSLDKNRPIRNPENWHRDDIDSNIPNPLRFLRAGEYSKFPYSVMEIKVINQDNSQMPNYEWIKDLTNSHLVNEV PKFSLYLQGVASLFGEDDKYVNILPFWLPDLETDIRKNPQEAYEEEKKTLQKQKSIHDKLDNMRRLSKISVPDGKTTERQ GQKDQNTRHVIADLEDHESSDEEGTALPKKSAVKKGKKFKTNAAFLKILAGKNISENGNDPYSDDTDSASSFQLPPGVKK PVHLLKNAGPVKVEAKVWLANERTFNRWLSVTTLLSVLTFSIYNSVQKAEFPQLADLLAYVYFFLTLFCGVWAYRTYLKR LTLIKGRSGKHLDAPVGPILVAVVLIVTLVVNFSVAFKEAARRE Q02725 Vacuole transporter chaperone complex subunit 5 Saccharomyces cerevisiae S288C ATCC 204508 / S288c 0.012434865 1 Homo sapiens LEVO SIYEYRHDEVVTFLYLSALLTSCIMASVCLGIVLSLFRGQSNNEIDLEIQNILIAIIIISLLVSLILICACLLLLFSRFT LAPIWHYVGCFTMFFSVTGTVCYGMIEIFF P38966 INOSITOL HEXAKISPHOSPHATE 0.0006600349999999999 3 IHP singleParticle particle 8.0 150.0 NaCl sodium chloride 25.0 Tris-HCL Tris Base 0.0002 GDN Glyco-diosgenin 1.0 IP6 Phytic Acid

150mM NaCl, 25mM Tris-HCL, 0.0002m/v GDN, 1mM IP6

ETHANE 100 281 FEI VITROBOT MARK IV TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.8 2.2 130000.0 FEI FALCON IV (4k x 4k) 4096 4096 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 3.04 FSC 0.143 CUT-OFF 186553 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_64273.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 297.6 297.6 297.6 90.0 90.0 90.0 X Y Z -0.028052596 1.9737426 0.0008871567 0.020235939 0.93 0.93 0.93 0.0915 AUTHOR ::::EMDATABANK.org::::EMD-64273:::: emd_64273_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 297.6 297.6 297.6 90.0 90.0 90.0 X Y Z -0.41342667 0.68344164 0.00057240215 0.02820592 0.93 0.93 0.93 AUTHOR ::::EMDATABANK.org::::EMD-64273:::: emd_64273_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 297.6 297.6 297.6 90.0 90.0 90.0 X Y Z -0.41164213 0.6647218 0.0005709063 0.028376302 0.93 0.93 0.93 AUTHOR ::::EMDATABANK.org::::EMD-64273::::