<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_11_0/emdb.xsd" version="3.0.11.0" emdb_id="EMD-64365">
    <admin>
        <current_status>
            <date>2026-03-25</date>
            <code>REL</code>
            <processing_site>PDBc</processing_site>
        </current_status>
        <revision_history>
            <revision version="1.0" date="2026-03-25">
                <change_list>
                    <metadata>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </metadata>
                    <additional_map part="1">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </additional_map>
                    <fsc>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </fsc>
                    <half_map part="1">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </half_map>
                    <half_map part="2">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </half_map>
                    <image>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </image>
                    <mask part="1">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </mask>
                    <primary_map>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </primary_map>
                </change_list>
            </revision>
        </revision_history>
        <sites>
            <deposition>PDBj</deposition>
            <last_processing>PDBc</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-04-24</deposition>
            <header_release>2026-03-25</header_release>
            <map_release>2026-03-25</map_release>
            <update>2026-03-25</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <country>China</country>
            </grant_reference>
        </grant_support>
        <title>mouse PDCD5-TRiC complex</title>
        <authors_list>
            <author>Song QQ</author>
            <author>Cong Y</author>
        </authors_list>
        <keywords>Complex, cofactor, CHAPERONE</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Wei H</author>
                    <author order="2">Song Q</author>
                    <author order="3">Wang L</author>
                    <author order="4">Deng Q</author>
                    <author order="5">Wu B</author>
                    <author order="6">Chen Y</author>
                    <author order="7">Han T</author>
                    <author order="8">Guo Y</author>
                    <author order="9">Li Z</author>
                    <author order="10">Dong F</author>
                    <author order="11">Ma S</author>
                    <author order="12">Zhao Q</author>
                    <author order="13">Shi X</author>
                    <author order="14">Pan C</author>
                    <author order="15">Jiang W</author>
                    <author order="16">Liu X</author>
                    <author order="17">Chen Y</author>
                    <author order="18">Jiao R</author>
                    <author order="19">Yuan L</author>
                    <author order="20">Liu C</author>
                    <author order="21">Guo X</author>
                    <author order="22">Cong Y</author>
                    <author order="23">Li W</author>
                    <title>PDCD5 promotes substrate release from the TRiC complex in cilia and flagella.</title>
                    <journal_abbreviation>Mol.Cell</journal_abbreviation>
                    <country>US</country>
                    <volume>86</volume>
                    <first_page>376</first_page>
                    <last_page>392.e11</last_page>
                    <year>2026</year>
                    <external_references type="PUBMED">41506263</external_references>
                    <external_references type="DOI">doi:10.1016/j.molcel.2025.12.012</external_references>
                    <external_references type="ISSN">1097-2765</external_references>
                    <external_references type="CSD">2168</external_references>
                    <external_references type="ASTM">MOCEFL</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-64335</emdb_id>
                <relationship>
                    <other>consensus EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-64335</accession_id>
                <content_type>consensus EM volume</content_type>
            </db_reference>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-64365</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>mouse PDCD5-TRiC complex</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>mouse PDCD5-TRiC complex</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">9.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">15.0</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 BIOQUANTUM (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">57.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
                <startup_model type_of_model="EMDB MAP">
                    <emdb_id>EMD-32922</emdb_id>
                </startup_model>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">3.55</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>659930</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>RANDOM ASSIGNMENT</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="256001">
        <file>emd_64365.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>400</col>
            <row>400</row>
            <sec>400</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>400</x>
            <y>400</y>
            <z>400</z>
        </spacing>
        <cell>
            <a units="Å">356.0</a>
            <b units="Å">356.0</b>
            <c units="Å">356.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.122232236</minimum>
            <maximum>15.172231</maximum>
            <average>0.018497322</average>
            <std>0.56861216</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">0.89</x>
            <y units="Å">0.89</y>
            <z units="Å">0.89</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>2.06</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-64365::::</label>
    </map>
    <interpretation>
        <segmentation_list>
            <segmentation>
                <file>emd_64365_msk_1.map</file>
            </segmentation>
        </segmentation_list>
        <additional_map_list>
            <additional_map format="CCP4" size_kbytes="256001">
                <file>emd_64365_additional_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>400</col>
                    <row>400</row>
                    <sec>400</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>400</x>
                    <y>400</y>
                    <z>400</z>
                </spacing>
                <cell>
                    <a units="Å">356.0</a>
                    <b units="Å">356.0</b>
                    <c units="Å">356.0</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.13441767</minimum>
                    <maximum>0.3348127</maximum>
                    <average>0.00015774068</average>
                    <std>0.012445124</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">0.89</x>
                    <y units="Å">0.89</y>
                    <z units="Å">0.89</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-64365::::</label>
            </additional_map>
        </additional_map_list>
        <half_map_list>
            <half_map format="CCP4" size_kbytes="256001">
                <file>emd_64365_half_map_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>400</col>
                    <row>400</row>
                    <sec>400</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>400</x>
                    <y>400</y>
                    <z>400</z>
                </spacing>
                <cell>
                    <a units="Å">356.0</a>
                    <b units="Å">356.0</b>
                    <c units="Å">356.0</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.393743</minimum>
                    <maximum>0.4501167</maximum>
                    <average>0.00063886045</average>
                    <std>0.04453654</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">0.89</x>
                    <y units="Å">0.89</y>
                    <z units="Å">0.89</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-64365::::</label>
            </half_map>
            <half_map format="CCP4" size_kbytes="256001">
                <file>emd_64365_half_map_2.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>400</col>
                    <row>400</row>
                    <sec>400</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>400</x>
                    <y>400</y>
                    <z>400</z>
                </spacing>
                <cell>
                    <a units="Å">356.0</a>
                    <b units="Å">356.0</b>
                    <c units="Å">356.0</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.35930964</minimum>
                    <maximum>0.45173764</maximum>
                    <average>0.0006352419</average>
                    <std>0.044405513</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">0.89</x>
                    <y units="Å">0.89</y>
                    <z units="Å">0.89</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-64365::::</label>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
