2025-11-19 REL PDBj INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _em_admin.last_update PDBj PDBj 2025-04-24 2025-11-05 2025-11-05 2025-11-19 National Research Foundation (NRF, Korea) NRF-2022R1A5A10313611 Korea, Republic Of Cryo-EM structure of human OAT1 in complex with olmesartan and bromide ion. Jeon HM Eun J Kim KH Kim Y drug-drug interaction, transporter, substrate, organic anion., MEMBRANE PROTEIN Jeon HM Eun J Kim KH Kim Y Cryo-EM structures of human OAT1 reveal drug binding and inhibition mechanisms. Structure UK 33 1856 1866.e5 2025 40845848 doi:10.1016/j.str.2025.07.019 0969-2126 2005 STRUE6 9unx FULLOVERLAP EMDB EMD-64368 associated EM volume
Cryo-EM structure of human OAT1 in complex with olmesartan and bromide ion.
 Human OAT1 in complex with olmesartan and bromide Human OAT1 in complex with olmesartan and bromide 0 1
human OAT1 in complex with olmesartan and, adopting inward facing conformation and embedded in LMNG/CHS micelle
Homo sapiens kidney Plasma membrane 0.062 Solute carrier family 22 member 6 Homo sapiens Kidney Epithelial 0.061869027
human OAT1 in complex with olmesartan and bromide ion, adopting inward facing conformation and embedded in LMNG/CHS micelle
1 Homo sapiens LEVO MAFNDLLQQVGGVGRFQQIQVTLVVLPLLLMASHNTLQNFTAAIPTHHCRPPADANLSKNGGLEVWLPRDRQGQPESCLR FTSPQWGLPFLNGTEANGTGATEPCTDGWIYDNSTFPSTIVTEWDLVCSHRALRQLAQSLYMVGVLLGAMVFGYLADRLG RRKVLILNYLQTAVSGTCAAFAPNFPIYCAFRLLSGMALAGISLNCMTLNVEWMPIHTRACVGTLIGYVYSLGQFLLAGV AYAVPHWRHLQLLVSAPFFAFFIYSWFFIESARWHSSSGRLDLTLRALQRVARINGKREEGAKLSMEVLRASLQKELTMG KGQASAMELLRCPTLRHLFLCLSMLWFATSFAYYGLVMDLQGFGVSIYLIQVIFGAVDLPAKLVGFLVINSLGRRPAQMA ALLLAGICILLNGVIPQDQSIVRTSLAVLGKGCLAASFNCIFLYTGELYPTMIRQTGMGMGSTMARVGSIVSPLVSMTAE LYPSMPLFIYGAVPVAASAVTVLLPETLGQPLPDTVQDLESRWAPTQKEAGIYPRKGKQTRQQQEHQKYMVPLQASAQEK NGL Q4U2R8 BROMIDE ION 7.9904e-05 1 BR Olmesartan 0.000446502 1 OLM singleParticle particle 18 7.5 150.0 NaBr sodium bromide 20.0 C8H18N2O4S HEPES 20.0 KNaC4H4O6 sodium potassium tartrate
20 mM HEPES, 150 mM NaCl, 20 mM Na/K-tartrate, 0.0015% LMNG, 0.00015% CHS
 Quantifoil R1.2/1.3 GOLD 300 CARBON HOLEY GLOW DISCHARGE 0.025 ETHANE 100 283 FEI VITROBOT MARK IV
This sample is a complex formed by composition of human OAT1, Fab targeting C-terminus of human OAT1, and the substrate olmesartan, purified in bromide buffer.
 TFS KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 0.8 1.8 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER GATAN K3 BIOQUANTUM (6k x 4k) 5760 4092 3 32271 60.0 1 10849658 cryoSPARC 4.4.1 PHASE FLIPPING AND AMPLITUDE CORRECTION 1 C1 3.45 FSC 0.143 CUT-OFF cryoSPARC 4.4.1 118678 MAXIMUM LIKELIHOOD cryoSPARC 4.4.1 NOT APPLICABLE 3 58934.0 RELION 5.0 emd_64368.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 280 280 280 0 0 0 280 280 280 230.944 230.944 230.944 90.0 90.0 90.0 X Y Z -0.9568511 1.4021755 0.00039303894 0.025652524 0.8248 0.8248 0.8248 0.15 AUTHOR Cryo-EM map of human OAT1-olmesartan-bromide complex. 9KLZ A PDB experimental model RIGID BODY FIT emd_64368_msk_1.map emd_64368_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 280 280 280 0 0 0 280 280 280 230.944 230.944 230.944 90.0 90.0 90.0 X Y Z -0.37593302 0.7284237 0.0006745275 0.04714721 0.8248 0.8248 0.8248 AUTHOR Cryo-EM half map of human OAT1-olmesartan-bromide complex. emd_64368_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 280 280 280 0 0 0 280 280 280 230.944 230.944 230.944 90.0 90.0 90.0 X Y Z -0.38265228 0.67491007 0.00067725265 0.04690253 0.8248 0.8248 0.8248 AUTHOR Cryo-EM half map of human OAT1-olmesartan-bromide complex.