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    <admin>
        <current_status>
            <date>2026-03-25</date>
            <code>REL</code>
            <processing_site>PDBc</processing_site>
        </current_status>
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        <sites>
            <deposition>PDBj</deposition>
            <last_processing>PDBc</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-05-13</deposition>
            <header_release>2026-03-25</header_release>
            <map_release>2026-03-25</map_release>
            <update>2026-03-25</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Other government</funding_body>
                <country>China</country>
            </grant_reference>
        </grant_support>
        <title>CryoEM Structure of LmuAB-DNA complex</title>
        <authors_list>
            <author>Li M</author>
            <author>Zhao X</author>
            <author>An L</author>
            <author>Li S</author>
            <author>Zhang K</author>
            <author>Feng Y</author>
            <author>Zhang K</author>
            <author>Chang C</author>
            <author>Li S</author>
            <author>Li S</author>
        </authors_list>
        <keywords>complex, anti-phage system, IMMUNE SYSTEM</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0009-0006-8994-2337" order="1">Li M</author>
                    <author ORCID="0009-0005-7144-3989" order="2">Zhao X</author>
                    <author ORCID="0009-0008-3213-0295" order="3">Zhao X</author>
                    <author ORCID="0000-0002-5687-7501" order="4">Li D</author>
                    <author ORCID="0009-0005-2835-4155" order="5">Xiong W</author>
                    <author ORCID="0009-0006-8420-6103" order="6">Gao Z</author>
                    <author order="7">Huang L</author>
                    <author order="8">An L</author>
                    <author order="9">Gao Y</author>
                    <author ORCID="0000-0002-7041-5960" order="10">Li S</author>
                    <author ORCID="0000-0002-1576-1385" order="11">Feng Y</author>
                    <author ORCID="0000-0003-0414-4776" order="12">Zhang K</author>
                    <author ORCID="0000-0002-8702-6005" order="13">Zhang Y</author>
                    <title>Structural insights into type-I and type-II Lamassu antiphage systems.</title>
                    <journal_abbreviation>Nat.Chem.Biol.</journal_abbreviation>
                    <country>US</country>
                    <year>2026</year>
                    <external_references type="PUBMED">41482579</external_references>
                    <external_references type="DOI">doi:10.1038/s41589-025-02102-z</external_references>
                    <external_references type="ISSN">1552-4469</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>9ux7</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-64575</accession_id>
                <content_type>associated EM volume</content_type>
                <details>CryoEM Structure of LmuAB-DNA complex</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>CryoEM structure of LmuAB-DNA complex</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>CryoEM structure of LmuAB-DNA complex</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="1761765">Bacillus sp. (strain NCIM 5461 / CCTCC AB 2011126 / NIO-1130)</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.18</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Lamassu protein LmuB</name>
                <natural_source database="NCBI">
                    <organism ncbi="1761765">Bacillus sp. nio-1130</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.066507977</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MIIIAFSILDFKNKEAQNFDFKAGTNLIVSKGNTKGKSSLLKSMYYTLGFDVHQFPSNWNINFMYFQIEVLINNVKYNIT
RQKNIFRVSDVEVPLNVKEYSEWLQHKLEIKMQLANTHTKHLYEAYSSAVILPFYIDQDDSWDGGIYRNVTNTLNQYTRI
PADIFKSVFNLSNYELLELQNSLTNYSKEKNTVVSTIKSLLNVLEDYRHENADVPTVSKIDKIALNKDIDRYLQMQNELN
EQIVKYKMKLLNKQEMLDLQKQELSELEQLLKMNKKRYNSIETECQYCHSKLTKEQSLTRLDLSNNYFEISLLKEEIEKE
VVKLTNEIIIFESQQNSIESKIDEIHRRIQNSKDLLTIDDYVKATAKKEASNELESLVDKQVLSKYNLEEKIKVLRREIN
KLKKEKESLREIIERDYTDLVFEIKKVLNDLNDTKLDLSELNLDELKFLEFKKISGSGMDKNKKFLAYYLIYFSLLRKYS
SYIIPFCMDSFIKNEITGETAKKMFEAIEKYFFDTNQSFFSIVSENLKHLEFVDSYNKINVEGKLLVRDKYDEIALKFKF
DS</string>
                    <external_references type="UNIPROTKB">P0DW44</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Lamassu protein LmuA</name>
                <natural_source database="NCBI">
                    <organism ncbi="1761765">Bacillus sp. nio-1130</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.036103792999999995</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MIEIDNGGAIALKGFNYQKASIILVMIHNFEKDNFIVIPESQEDFEIHLGQDTYFIQVKGTKKLSIGKLKSRPSGKASII
EKNLSPGNVGDIRKIFLWDIAELTKNELISQEGTLIPMKHSLSLKQKTEIINTLDLDEEQKNRMNNQYIYITPFPNDINL
ALTFLKGEMVNENLLVSNDRAKLVLGELSLEIDRKSEIVVSTESDVERKKIDGNYLKQVFINIKQKEMFDEILDNLSINT
IMKKKVKKEKLRIPLLYQNIKEQTKQKADINLLMRENDEGAINYLRDLLVEIVPDMKPTELSIALAIDCFCELGE</string>
                    <external_references type="UNIPROTKB">P0DW43</external_references>
                </sequence>
            </protein_or_peptide>
            <dna macromolecule_id="3">
                <name>DNA (41-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.012594122</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <sequence>
                    <string>(DC)(DG)(DC)(DT)(DT)(DT)(DA)(DT)(DC)(DA)(DG)(DA)(DA)(DG)(DC)(DC)(DA)(DG)(DA)(DC)
(DA)(DT)(DT)(DA)(DA)(DC)(DG)(DC)(DT)(DT)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DA)(DA)(DC)
(DT)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <dna macromolecule_id="4">
                <name>DNA (41-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.012647128</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <sequence>
                    <string>(DA)(DG)(DT)(DT)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(DA)(DA)(DG)(DC)(DG)(DT)(DT)(DA)(DA)
(DT)(DG)(DT)(DC)(DT)(DG)(DG)(DC)(DT)(DT)(DC)(DT)(DG)(DA)(DT)(DA)(DA)(DA)(DG)(DC)
(DG)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
        </macromolecule_list>
    </sample>
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        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">5.0</concentration>
                    <buffer>
                        <ph>8.0</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">1.6</nominal_defocus_min>
                    <nominal_defocus_max units="µm">3.1</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">50.4</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>1144023</number_selected>
                </particle_selection>
                <ctf_correction>
                    <type>NONE</type>
                </ctf_correction>
                <startup_model type_of_model="NONE"/>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">3.73</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                            <version>4.2</version>
                        </software>
                    </software_list>
                    <number_images_used>437756</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
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